Zobrazeno 1 - 10
of 230
pro vyhledávání: '"Herbert Schneckenburger"'
Publikováno v:
Biophysica, Vol 4, Iss 2, Pp 142-157 (2024)
For many biomedical applications, laser-assisted methods are essential to enhance the three-dimensional (3D) resolution of a light microscope. In this report, we review possibilities to improve the 3D imaging potential by axial tomography. This metho
Externí odkaz:
https://doaj.org/article/875b923c9e2f422289839a09647428d9
Autor:
Herbert Schneckenburger
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 10, p 8526 (2023)
Since their invention by Theodore Maiman in 1960, lasers represent a class of light sources based on the stimulated emission of radiation in the visible, ultraviolet or infrared spectral range [...]
Externí odkaz:
https://doaj.org/article/41e25094d9c0439abc807aba3d838caf
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 11, p 6277 (2022)
Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM) is applied in view of early detection of cellular responses to the cytostatic drug doxorubicin. Therefore, we determined cell-substrate topology of cultivated CHO cells trans
Externí odkaz:
https://doaj.org/article/733651380efd4590a7cffcbf6a0b45e4
Autor:
Herbert Schneckenburger
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 9, p 5015 (2022)
Due to their unique properties—coherent radiation, diffraction limited focusing, low spectral bandwidth and in many cases short light pulses—lasers play an increasing role in live cell microscopy. Lasers are indispensable tools in 3D microscopy,
Externí odkaz:
https://doaj.org/article/3f9cb55466b04762a791b98060678f8d
Publikováno v:
Photonics, Vol 8, Iss 7, p 275 (2021)
A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of w
Externí odkaz:
https://doaj.org/article/e327b0f34549477f9d87ac05d954d3b6
Publikováno v:
Photonics, Vol 8, Iss 6, p 176 (2021)
For probing small distances in living cells, methods of super-resolution microscopy and molecular sensing are reported. A main requirement is low light exposure to maintain cell viability and to avoid photobleaching of relevant fluorophores. From thi
Externí odkaz:
https://doaj.org/article/e607b438bbcf4e67b51bd08eaa6cc026
Publikováno v:
Applied Sciences, Vol 11, Iss 2, p 733 (2021)
Methods and applications of light microscopy in the life sciences are compared with respect to 3D imaging, resolution, light exposure, sensitivity, and recording time. While conventional wide-field or laser scanning microscopy appear appropriate for
Externí odkaz:
https://doaj.org/article/398b0011765141c895e2e859e1cc8ef4
Autor:
Verena Stadlbauer, Peter Lanzerstorfer, Cathrina Neuhauser, Florian Weber, Flora Stübl, Petra Weber, Michael Wagner, Birgit Plochberger, Stefan Wieser, Herbert Schneckenburger, Julian Weghuber
Publikováno v:
International Journal of Molecular Sciences, Vol 21, Iss 21, p 7964 (2020)
Due to the global rise of type 2 diabetes mellitus (T2DM) in combination with insulin resistance, novel compounds to efficiently treat this pandemic disease are needed. Screening for compounds that induce the translocation of glucose transporter 4 (G
Externí odkaz:
https://doaj.org/article/fb2414b7966743c995cae17d07283d2e
Publikováno v:
International Journal of Molecular Sciences, Vol 21, Iss 19, p 7099 (2020)
Here, we present a simple and robust experimental setup for the super-resolution live cell microscopy of membrane-proximal fluorophores, which is comparably easy to perform and to implement. The method is based on Structured Illumination Microscopy (
Externí odkaz:
https://doaj.org/article/ec4001e8ec1e4ce49501f9683c9b8e8b
Autor:
Petra Weber, Sarah Schickinger, Michael Wagner, Brigitte Angres, Thomas Bruns, Herbert Schneckenburger
Publikováno v:
International Journal of Molecular Sciences, Vol 16, Iss 3, Pp 5375-5385 (2015)
Non-radiative cell membrane associated Förster Resonance Energy Transfer (FRET) from an enhanced cyan fluorescent protein (ECFP) to an enhanced yellow fluorescent protein (EYFP) is used for detection of apoptosis in 3-dimensional cell cultures. FRET
Externí odkaz:
https://doaj.org/article/c44c767e9d0c44429acce2f60773d3b3