Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Herbert Hottinger"'
Autor:
Sunil Kochhar, Victor S. Lamzin, Adelia Razeto, JacquesâEdouard Germond, Herbert Hottinger, Michèle Delley
Publikováno v:
European Journal of Biochemistry. 267:1633-1639
The role of three histidine residues (His205, His296 and His303) and Asp259, important for the catalysis of NAD+-specific D-lactate dehydrogenase, was investigated using site-directed mutagenesis. None of these residues is presumed to be involved in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45fdb867353a726b1923cb5357da0b6f
https://doi.org/10.1046/j.1432-1327.2000.01155.x
https://doi.org/10.1046/j.1432-1327.2000.01155.x
Autor:
Tony Atkinson, Paul G. Taylor, Nathalie Chuard, Herbert Hottinger, Michael D. Scawen, David J. Nicholls, Sunil Kochhar
Publikováno v:
European Journal of Biochemistry. 208:799-805
NAD(+)-dependent D-lactate dehydrogenase from Lactobacillus helveticus was purified to apparent homogeneity, and the sequence of the first 36 amino acid residues determined. Using forward and reverse oligonucleotide primers, based on the N-terminal s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7cc93bd8344920b34e6e280d7ee5978
https://doi.org/10.1111/j.1432-1033.1992.tb17250.x
https://doi.org/10.1111/j.1432-1033.1992.tb17250.x
Publikováno v:
Biochemical and Biophysical Research Communications. 184:60-66
A comparison of the primary structures of NAD(+)-dependent D-lactate dehydrogenase with L-lactate dehydrogenase and L-malate dehydrogenase failed to show any sequence similarity. However, D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48ee46a96d582a4a6375555cee408061
https://doi.org/10.1016/0006-291x(92)91157-l
https://doi.org/10.1016/0006-291x(92)91157-l
Publikováno v:
Journal of Biological Chemistry. 267:8499-8513
The NAD(+)-dependent D-lactate dehydrogenase was purified to apparent homogeneity from Lactobacillus bulgaricus and its complete amino acid sequence determined. Two gaps in the polypeptide chain (10 residues) were filled by the deduced amino acid seq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6576533d28802580ff2e7d7b7c67c440
https://doi.org/10.1016/s0021-9258(18)42473-8
https://doi.org/10.1016/s0021-9258(18)42473-8
Autor:
Adelia Razeto, Keith S. Wilson, Herbert Hottinger, Miroslava Dauter, Sunil Kochhar, Victor S. Lamzin
Publikováno v:
Journal of molecular biology. 318(1)
NAD-dependent Lactobacillus bulgaricus d -Lactate dehydrogenase ( d -LDHb) catalyses the reversible conversion of pyruvate into d -lactate. Crystals of d -LDHb complexed with NADH were grown and X-ray data collected to 2.2 A. The structure of d -LDHb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ac1c8f5a756a60be257e67dba99f21e
https://pubmed.ncbi.nlm.nih.gov/12054772
https://pubmed.ncbi.nlm.nih.gov/12054772
Publikováno v:
Biochimie. 76(1)
The nifS gene was first identified in nitrogen-fixing bacteria where its protein product is essential for efficient nitrogen fixation. Here, we demonstrate that a nifS-like gene also occurs in Lactobacillus bulgaricus, an organism which does not fix
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb2e4318c919d8ea15ab69d34e2f90a
https://pubmed.ncbi.nlm.nih.gov/8031904
https://pubmed.ncbi.nlm.nih.gov/8031904
Publikováno v:
The Journal of biological chemistry. 267(28)
Recently, we amplified the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene by the polymerase chain reaction, cloned and overexpressed it in Escherichia coli (Kochhar, S., Chuard, N., and Hottinger, H. (1992) Biochem. Biophys. R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bb88577e51ea5a08427dbcba05541c0
https://pubmed.ncbi.nlm.nih.gov/1356978
https://pubmed.ncbi.nlm.nih.gov/1356978
Publikováno v:
Biochemical and biophysical research communications. 185(2)
The Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene was amplified by the polymerase chain reaction and cloned into an Escherichia coli expression plasmid pKK223.3. Attempts to clone the full-length chromosomal DNA encoding D-la
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6998cc67385125fc13002929b16cab1f
https://pubmed.ncbi.nlm.nih.gov/1610363
https://pubmed.ncbi.nlm.nih.gov/1610363
Publikováno v:
Applied and environmental microbiology. 56(6)
From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d562a5b65781af90aca8da961129c665
https://pubmed.ncbi.nlm.nih.gov/16348233
https://pubmed.ncbi.nlm.nih.gov/16348233
Publikováno v:
Genetics. 87:471-489
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore and mitotic analysis. A new diploidization method ut
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b3426c4838a2b9c726c134c45c2febe
https://doi.org/10.1093/genetics/87.3.471
https://doi.org/10.1093/genetics/87.3.471