The Quiet Embryo Hypothesis: 20 years on

Autor: Henry J. Leese, Daniel R. Brison, Roger G. Sturmey

Publikováno v: Frontiers in Physiology, Vol 13 (2022)

This article revisits the hypothesis, proposed in 2002, that the successful development of oocytes and preimplantation mammalian embryos is associated with a metabolism which is “quiet” rather than “active”, within limits which had yet to be

Externí odkaz: https://doaj.org/article/a211ce04bc344a5abf0f091c2657d054

Glucose concentration during equine in vitro maturation alters mitochondrial function

Autor: Henry J. Leese, Niamh Lewis, Katrin Hinrichs, Roger G. Sturmey, Daniel R Brison, Caroline McGregor Argo

Publikováno v: Lewis, N, Hinrichs, K, Leese, H J, McGregor Argo, C, Brison, D R & Sturmey, R G 2020, ' Glucose concentration during equine in vitro maturation alters mitochondrial function ', Reproduction, vol. 160, no. 2, pp. 227-237 . https://doi.org/10.1530/REP-20-0032

The use of in vitro embryo production in the horse is increasing in clinical and research settings; however, protocols are yet to be optimised. Notably, the two most commonly used base media for in vitro maturation (IVM) supply glucose at markedly di

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33a45fb5e1c286ed3a5cf2245639a4e9
https://doi.org/10.1530/rep-20-0032

Amino Acids and the Early Mammalian Embryo: Origin, Fate, Function and Life-Long Legacy

Autor: Roger G. Sturmey, Paul J. McKeegan, Henry J. Leese

Publikováno v: International Journal of Environmental Research and Public Health
International Journal of Environmental Research and Public Health, Vol 18, Iss 9874, p 9874 (2021)

Amino acids are now recognised as having multiple cellular functions in addition to their traditional role as constituents of proteins. This is well-illustrated in the early mammalian embryo where amino acids are now known to be involved in intermedi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a78d3a5d78330d04d989365443989ca
http://europepmc.org/articles/PMC8467587

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos

Autor: Daniel R Brison, Roger G. Sturmey, Bethany Muller, Henry J. Leese, Niamh Lewis, Tope Adeniyi

Publikováno v: Scientific Reports
Muller, B, Lewis, N, Adeniyi, T, Leese, H J, Brison, D R & Sturmey, R G 2019, ' Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos ', Scientific Reports, vol. 9, no. 1, pp. 16778 . https://doi.org/10.1038/s41598-019-53066-9
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)

1.AbstractBackgroundMitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01cc81bb47a5aff78541be250156cd25
https://doi.org/10.1038/s41598-019-53066-9

Biological optimization, the Goldilocks principle, and how much islagomin the preimplantation embryo

Autor: Daniel R. Brison, Victoria Allgar, F. Guerif, Roger G. Sturmey, Henry J. Leese, Kersti Lundin

Publikováno v: Molecular Reproduction and Development. 83:748-754

The quiet embryo hypothesis postulates that early embryo viability is associated with a relatively low metabolism (Leese, 2002 BioEssays 24: 845-849). This proposal is re-visited here using retrospective and prospective data on the metabolic activity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::96f7f655cc13681e337db903b1856396
https://doi.org/10.1002/mrd.22684