Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Henry Hebel"'
Non-viral gene transfer using plasmid DNA (pDNA) is generally acknowledged as safe and non-immunogenic compared with the use of viral vectors. However, pre-clinical and clinical studies have shown that non-viral (lipoplex) gene transfer to the lung c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f2549edb3025dd33e63bce4eb7e41fd
https://doi.org/10.1016/j.biomaterials.2011.08.092
https://doi.org/10.1016/j.biomaterials.2011.08.092
Publikováno v:
Vaccine. 24:4607-4614
We have developed three major technologies that allow plasmid-based products to be used for large-scale vaccination or therapeutic protein applications. Our team has integrated these components into one complete, cost-effective, easy-to-use system ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f5972dc6b670e3d1dabfc9724baf3b1
https://doi.org/10.1016/j.vaccine.2005.08.049
https://doi.org/10.1016/j.vaccine.2005.08.049
Autor:
Stephen C. Hyde, Deborah R. Gill, Seng H. Cheng, Ron Scheule, Henry Hebel, Lee A. Davies, Graciela A Nunez-Alonso
Publikováno v:
BioTechniques. 49(3)
Nonviral gene therapy utilizing plasmid DNA (pDNA) complexed with cationic lipids (lipoplexes) or cationic polymers (polyplexes) has demonstrated considerable potential for the treatment of a variety of diseases. However, progress toward clinical app
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8bb51dcd677e673a852f880f7a28717
https://pubmed.ncbi.nlm.nih.gov/20854270
https://pubmed.ncbi.nlm.nih.gov/20854270
Autor:
Ying Cai, Robert J. Juba, Henry Hebel, Ruxandra Draghia-Akli, Stephen Rodriguez, Ramu Rameswaran
Publikováno v:
Vaccine. 28(8)
The increased use of plasmid-based vaccines to replace their more challenging viral counterparts has increased the demand for high purity and high concentration plasmids. Here we report the production of plasmids encoding different transgenes for DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c621942e10aa1f0d68b70c1589b26d58
https://pubmed.ncbi.nlm.nih.gov/19896448
https://pubmed.ncbi.nlm.nih.gov/19896448
Publikováno v:
Expert review of vaccines. 8(9)
The demand for plasmid DNA in large quantities at high purity and concentration is expected to escalate as more DNA vaccines are entering clinical trial status and becoming closer to market approval. This review outlines different methods for DNA vac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8af9398ed8191b7efb0eef2301806dc2
https://pubmed.ncbi.nlm.nih.gov/19722898
https://pubmed.ncbi.nlm.nih.gov/19722898
Publikováno v:
European Journal of Molecular and Clinical Medicine, Vol 2, Iss 2, Pp 56-57 (2015)
Background Ionizing radiation induces lipid peroxidation and forms reactive oxygen species (ROS) within the cell. Due to their highly reactive state, ROS have short diffusion distances and quickly transfer unbalanced electrons to neighboring molecule
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2219f745a04044d72d2ba01e50e8f445
https://doi.org/10.1016/j.nhtm.2014.11.007
https://doi.org/10.1016/j.nhtm.2014.11.007
Publikováno v:
Molecular Therapy. 11:S221-S222
Top of pageAbstract Introduction: As the number of gene therapy studies and trials has increased in the past few years, an ever-increasing demand for plasmid DNA-based products exists. In this expanding field of plasmid production, emphasis has alway
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::543b860dc9b272eb8d799e970b5d1a4d
https://doi.org/10.1016/j.ymthe.2005.07.114
https://doi.org/10.1016/j.ymthe.2005.07.114
Publikováno v:
Molecular Therapy. 9:S310-S311
Cost-effective production of commercial-scale batches of pharmaceutical-grade plasmid DNA remains a challenge for the maturing industry of plasmid-based biologics. A process is required that meets the quality standards for human pharmaceutical produc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9da8c1c2823cd88f58e0d1c2081d24a6
https://doi.org/10.1016/j.ymthe.2004.06.718
https://doi.org/10.1016/j.ymthe.2004.06.718
Autor:
Justin Miller, Jeffrey Darnell, Gary Gates, Sriram Ramakrishnan, Hugo L. Gonzales, Henry Hebel
Publikováno v:
Molecular Therapy. 13:S121
The demand for high purity plasmid DNA for use in the fields of gene therapy and DNA vaccination is escalating rapidly. The new and expanding field of RNAi also relies on plasmids to express short double-stranded RNA molecules that specifically targe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccff3a7b0bd72d49ba7eb7cdb6d95c7d
https://doi.org/10.1016/j.ymthe.2006.08.374
https://doi.org/10.1016/j.ymthe.2006.08.374
Publikováno v:
Molecular Therapy. 11:S71
Adequate work has been performed on fermentation process optimizations for the production of plasmid DNA. However, the effects of dissolved oxygen (DO) control parameters in fermenters have not been addressed. DO control in high cell density fermenta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6942d7c220b59e74e49301fea7b5acd5
https://doi.org/10.1016/j.ymthe.2005.06.184
https://doi.org/10.1016/j.ymthe.2005.06.184