Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Henrik Seir Thoke"'
Autor:
Henrik Seir Thoke, Asger Tobiesen, Jonathan Brewer, Per Lyngs Hansen, Roberto P Stock, Lars F Olsen, Luis A Bagatolli
Publikováno v:
PLoS ONE, Vol 10, Iss 2, p e0117308 (2015)
We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogene
Externí odkaz:
https://doaj.org/article/edfb4d577a5642818d75f9ce41bf375a
Autor:
Luis A. Bagatolli, Roberto P. Stock, Jesús Lara-Popoca, Henrik Seir Thoke, Enrique Rudiño-Piñera
Publikováno v:
Lara-Popoca, J, Thoke, H S, Stock, R P, Rudino-Pinera, E & Bagatolli, L A 2020, ' Inductive effects in amino acids and peptides : Ionization constants and tryptophan fluorescence ', Biochemistry and Biophysics Reports, vol. 24, 100802 . https://doi.org/10.1016/j.bbrep.2020.100802
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Consejo Nacional de Investigaciones Científicas y Técnicas
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Biochemistry and Biophysics Reports, Vol 24, Iss, Pp 100802-(2020)
Biochemistry and Biophysics Reports
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Biochemistry and Biophysics Reports, Vol 24, Iss, Pp 100802-(2020)
Biochemistry and Biophysics Reports
Although inductive effects in organic compounds are known to influence chemical properties such as ionization constants, their specific contribution to the properties/behavior of amino acids and functional groups in peptides remains largely unexplore
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f892a4172e3136d31bb8f048bf955b5f
https://findresearcher.sdu.dk:8443/ws/files/173606103/1_s2.0_S2405580820301126_main.pdf
https://findresearcher.sdu.dk:8443/ws/files/173606103/1_s2.0_S2405580820301126_main.pdf
Autor:
Roberto P. Stock, Luis A. Bagatolli, Sigmundur Thorsteinsson, Henrik Seir Thoke, Lars Folke Olsen
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
Thoke, H S, Thorsteinsson, S, Stock, R P, Bagatolli, L & Olsen, L F 2017, ' The dynamics of intracellular water constrains glycolytic oscillations in Saccharomyces cerevisiae ', Scientific Reports, vol. 7, 16250 . https://doi.org/10.1038/s41598-017-16442-x
Scientific Reports
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Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Thoke, H S, Thorsteinsson, S, Stock, R P, Bagatolli, L & Olsen, L F 2017, ' The dynamics of intracellular water constrains glycolytic oscillations in Saccharomyces cerevisiae ', Scientific Reports, vol. 7, 16250 . https://doi.org/10.1038/s41598-017-16442-x
Scientific Reports
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
We explored the dynamic coupling of intracellular water with metabolism in yeast cells. Using the polarity-sensitive probe 6-acetyl-2-dimethylaminonaphthalene (ACDAN), we show that glycolytic oscillations in the yeast S. cerevisiae BY4743 wild-type s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8139c3488a46ff83a508273ab8796f0
http://link.springer.com/article/10.1038/s41598-017-16442-x
http://link.springer.com/article/10.1038/s41598-017-16442-x
Autor:
Olaf-Georg Issinger, Lars Folke Olsen, Jennifer Hochscherf, Mai Bay Stie, Barbara Guerra, Henrik Seir Thoke
Publikováno v:
Stie, M B, Thoke, H S, Issinger, O-G, Hochscherf, J, Guerra, B & Olsen, L F 2019, ' Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells ', Colloids and Surfaces B: Biointerfaces, vol. 174, pp. 216-223 . https://doi.org/10.1016/j.colsurfb.2018.11.005
Stie, M B, Thoke, H S, Issinger, O G, Hochscherf, J, Guerra, B & Olsen, L F 2019, ' Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells ', Colloids and Surfaces B: Biointerfaces, vol. 174, pp. 216-223 . https://doi.org/10.1016/j.colsurfb.2018.11.005
Stie, M B, Thoke, H S, Issinger, O G, Hochscherf, J, Guerra, B & Olsen, L F 2019, ' Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells ', Colloids and Surfaces B: Biointerfaces, vol. 174, pp. 216-223 . https://doi.org/10.1016/j.colsurfb.2018.11.005
We have successfully encapsulated two proteins, bovine serum albumin (BSA) and p53, in chitosan-tripolyphosphate (TPP) nanoparticles at various pH values from 5.5 to 6.5 and delivered the particles to human melanoma cells. The particles have diameter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::993025f1780c6966f9c519f511b96070
https://findresearcher.sdu.dk:8443/ws/files/145485847/Delivery_of_proteins_encapsulated_in_chitosan_tripolyphosphate_nanoparticles_to_human_skin_melanoma_cells.pdf
https://findresearcher.sdu.dk:8443/ws/files/145485847/Delivery_of_proteins_encapsulated_in_chitosan_tripolyphosphate_nanoparticles_to_human_skin_melanoma_cells.pdf
Publikováno v:
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Thoke, H S, Bagatolli, L A & Olsen, L F 2018, ' Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast ', Integrative Biology, vol. 10, no. 10, pp. 587-597 . https://doi.org/10.1039/C8IB00099A
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Thoke, H S, Bagatolli, L A & Olsen, L F 2018, ' Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast ', Integrative Biology, vol. 10, no. 10, pp. 587-597 . https://doi.org/10.1039/C8IB00099A
Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b7198ddf9fbfc1b7038a405098144ed
https://pubmed.ncbi.nlm.nih.gov/30176029
https://pubmed.ncbi.nlm.nih.gov/30176029
Autor:
Luis A. Bagatolli, Henrik Seir Thoke, Roberto P. Stock, Lars Duelund, Lars Folke Olsen, Thomas Heimburg
Publikováno v:
Thoke, H S, Olsen, L F, Duelund, L, Stock, R P, Heimburg, T & Bagatolli, L 2018, ' Is a constant low-entropy process at the root of glycolytic oscillations? ', Journal of Biological Physics, vol. 44, no. 3, pp. 419-431 . https://doi.org/10.1007/s10867-018-9499-2
We measured temporal oscillations in thermodynamic variables such as temperature, heat flux, and cellular volume in suspensions of non-dividing yeast cells which exhibit temporal glycolytic oscillations. Oscillations in these variables have the same
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7644b71c6f6f3748137ff865611ce8d9
http://link.springer.com/10.1007/s10867-018-9499-2
http://link.springer.com/10.1007/s10867-018-9499-2
Publikováno v:
Brewer, J R, Thoke, H S, Stock, R & Bagatolli, L A 2017, ' Enzymatic studies on planar supported membranes using a widefield fluorescence LAURDAN Generalized Polarization imaging approach ', Biochimica et Biophysica Acta-Biomembranes, vol. 1859, no. 5, pp. 888-895 . https://doi.org/10.1016/j.bbamem.2017.01.024
We introduce a custom-built instrument designed to perform fast LAURDAN Generalized Polarization (GP) imaging on planar supported membranes. It is mounted on a widefield fluorescence microscope and allows kinetic analysis of the GP function in the mi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::248a5dd8c1a4aa960c15e2a122922d79
https://pubmed.ncbi.nlm.nih.gov/28126480
https://pubmed.ncbi.nlm.nih.gov/28126480