Cell-Type-Specific High Throughput Toxicity Testing in Human Midbrain Organoids

Autor: Henrik Renner, Katharina J. Becker, Theresa E. Kagermeier, Martha Grabos, Farsam Eliat, Patrick Günther, Hans R. Schöler, Jan M. Bruder

Publikováno v: Frontiers in Molecular Neuroscience, Vol 14 (2021)

Toxicity testing is a crucial step in the development and approval of chemical compounds for human contact and consumption. However, existing model systems often fall short in their prediction of human toxicity in vivo because they may not sufficient

Externí odkaz: https://doaj.org/article/3fafc522595e473c9de7213b4e759c51

Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening

Autor: Henrik Renner, Mandy Otto, Martha Grabos, Hans Schöler, Jan Bruder

Publikováno v: Bio-Protocol, Vol 11, Iss 12 (2021)

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sec

Externí odkaz: https://doaj.org/article/034893d320c941ba94cc71c6acf9055b

Combining Automated Organoid Workflows with Artificial Intelligence-Based Analyses: Opportunities to Build a New Generation of Interdisciplinary High-Throughput Screens for Parkinson's Disease and Beyond

Autor: Hans R. Schöler, Henrik Renner, Jan M. Bruder

Publikováno v: Movement disorders : official journal of the Movement Disorder SocietyReferences. 36(12)

Parkinson's disease (PD) is the second most common neurodegenerative disease and primarily characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta of the midbrain. Despite decades of research and the development of var

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bef817ec20463a2e0738955678e5124e
https://pubmed.ncbi.nlm.nih.gov/34498298

Generation and Maintenance of Homogeneous Human Midbrain Organoids

Autor: Hans R. Schöler, Jan M. Bruder, Martha Grabos, Henrik Renner

Publikováno v: Bio Protoc

Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e244e6c32c45494037af42f1a4ac3f9
https://doi.org/10.21769/bioprotoc.4049

A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids

Autor: Sebastian A. Leidel, Hans R. Schöler, Yaroslav Tsytsyura, Stefan Peischard, Theresa E Kagermeier, Dagmar Zeuschner, Jie Wu, Jan M. Bruder, Guiscard Seebohm, Martha Grabos, Paul Disse, Katharina J Becker, Mandy Otto, Jürgen Klingauf, Henrik Renner

Publikováno v: eLife
eLife, Vol 9 (2020)
Renner, Henrik; Grabos, Martha; Becker, Katharina J; Kagermeier, Theresa E; Wu, Jie; Otto, Mandy; Peischard, Stefan; Zeuschner, Dagmar; TsyTsyura, Yaroslav; Disse, Paul; Klingauf, Jürgen; Leidel, Sebastian A; Seebohm, Guiscard; Schöler, Hans R; Bruder, Jan M (2020). A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids. eLife, 9 eLife Sciences Publications 10.7554/eLife.52904

Three-dimensional (3D) culture systems have fueled hopes to bring about the next generation of more physiologically relevant high-throughput screens (HTS). However, current protocols yield either complex but highly heterogeneous aggregates (‘organo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2af6cafe25b2e8749fddbcb7a904d019