Výsledky vyhledávání - "Henrik M. Pfundheller"

ProbeLibrary: A new method for faster design and execution of quantitative real-time PCR

Autor: Mikkel Noerholm, Peter Stein Nielsen, Niels Tolstrup, Henrik M. Pfundheller, Christian Lomholt, Nana Jacobsen, Peter Mouritzen

Publikováno v: Nature Methods. 2:313-316

ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide coverage in most organisms. These short probes are hig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dd8081b8ad83bad735a23fceb123e155
https://doi.org/10.1038/nmeth0405-313

Zobrazit plný text záznamu

The ProbeLibrary™-Expression profiling 99% of all human genes using only 90 dual-labeled real-time PCR Probes

Autor: Mikkel Noerholm, Niels B. Ramsing, Nana Jacobsen, Sakari Kauppinen, Niels Tolstrup, Christian Lomholt, Peter Stein Nielsen, Peter Mouritzen, Henrik M. Pfundheller

Publikováno v: BioTechniques. 37:492-495

While quantitative real-time RT-PCR is in principle a simple technique, the assay design remains fairly complex and designed assays often perform inadequately with respect to specificity, sensitivity, and reproducibility (1,2). The time spent on assa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::66b4e0daf6f22c3d1296c0314712fe32
https://doi.org/10.2144/04373af02

Zobrazit plný text záznamu

Improved Process for the Preparation of Nucleosidic Phosphoramidites Using a Safer and Cheaper Activator

Autor: Antonella Converso, Zhiqiang Guo, and Henrik M. Pfundheller, Yogesh S. Sanghvi

Publikováno v: Organic Process Research & Development. 4:175-181

A new, simplified commercial process for the preparation of nucleosidic phosphoramidites, key raw materials for the automated solid-supported synthesis of oligonucleotide-based drugs, was developed...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::255b39b82598160c758b114d7c3e6c65
https://doi.org/10.1021/op990086k

Zobrazit plný text záznamu

Oligonucleotides Containing Novel 4′-C- or 3′-C-(Aminoalkyl)-Branched Thymidines

Autor: Torsten Bryld, Henrik M. Pfundheller, Carl Erik Olsen, Jesper Wengel

Publikováno v: Helvetica Chimica Acta. 83:128-151

The synthesis of four novel 3′-C-branched and 4′-C-branched nucleosides and their transformation into the corresponding 3′-O-phosphoramidite building blocks for automated oligonucleotide synthesis is reported. The 4′-C-branched key intermedia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d3feeef068e93c240faba3a3f0b2a2c7
https://doi.org/10.1002/(sici)1522-2675(20000119)83:1<128::aid-hlca128>3.0.co

Zobrazit plný text záznamu

Evaluation of Oligonucleotides Containing Two Novel 2′-O-Methyl Modified Nucleotide Monomers: A 3′-C-Allyl and a 2′-O-3′-C-Linked Bicyclic Derivative

Autor: Alexei A. Koshkin, Carl Erik Olsen, Henrik M. Pfundheller, Jesper Wengel

Publikováno v: Nucleosides and Nucleotides. 18:2017-2030

The two ribo-configured nucleosides 1-(3-C-allyl-2-O-methyl-beta-D-ribo-pentofuranosyl)thymine 3 and (1S,5R,6R,8R)-5-hydroxy-6-(hydroxymethyl)-1-methoxy-8-(thymin-1-yl )- 2,7-dioxabicyclo[3.3.0]octane 6 have been transformed into their corresponding

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a45e127e03de6d231149a15b0041155
https://doi.org/10.1080/07328319908044861

Zobrazit plný text záznamu

Oligonucleotides containing 4′-C-aminomethyl-2′-modified thymidines show increased binding affinity towards DNA and RNA

Autor: Jesper Wengel, Henrik M. Pfundheller

Publikováno v: Bioorganic & Medicinal Chemistry Letters. 9:2667-2672

Oligonucleotides containing 4'-C-aminomethyl-2'-O-methyl or 4'-C-aminomethyl-2'-deoxy-2'-fluoro modified thymidines have been synthesized. Compared with the corresponding oligodeoxynucleotide reference these novel oligonucleotide analogues display in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aad6fa77bd4fbdd060822dd1d31b7c4
https://doi.org/10.1016/s0960-894x(99)00452-7

Zobrazit plný text záznamu

Synthesis of novel 3′-C-branched 2′-deoxynucleosides. Incorporation of 3′-C-(3-hydroxypropyl)thymidine into oligodeoxynucleotides

Autor: Sunil K. Sharma, Henrik M. Pfundheller, Pia N. Jørgensen, Poul Nielsen, Carl Erik Olsen, Marie Grimstrup, Claudia Ströch, Jesper Wengel, Garimella Viswanadham, Ulrik S. Sørensen

Publikováno v: Pfundheller, H M, Jørgensen, P N, Sørensen, U S, Sharma, S K, Grimstrup, M, Ströch, C, Nielsen, P, Viswanadham, G, Olsen, C E & Wengel, J 1998, ' Synthesis of novel 3'-C-branched 2'-deoxynucleosides. Incorporation of 3'-C-(3-hydroxypropyl)thymidine into oligodeoxynucleotides ', Royal Chemical Society. Journal. Perkin Transactions 1, vol. 1998, no. 8, pp. 1409-1422 . https://doi.org/10.1039/A708801I

The methyl glycoside derivatives 4, 6, 10 and 32 have been used as precursors for the synthesis of novel 3′-C-alkyl-modified α- and β-2′-deoxynucleosides. Using an alternative linear strategy, 3′-C-methyl- and 3′-C-azidomethyl-modified thym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::841b72974ff783104e6fe6b8047dc624
https://doi.org/10.1039/a708801i

Zobrazit plný text záznamu

Synthesis and Evaluation of Novel Oligodeoxynucleotides Containing 3′-C-(3-Benzoyloxypropyl)thymidine and Bicyclo Nucleoside Derivatives

Autor: Poul Nielsen, Jesper Wengel, Henrik M. Pfundheller

Publikováno v: Pfundheller, H M, Nielsen, P & Wengel, J 1997, ' Synthesis and Evaluation of Novel Oligodeoxynucleotides Containing 3′-C-(3-Benzoyloxypropyl)thymidine and Bicyclo Nucleoside Derivatives ', Nucleosides, Nucleotides and Nucleic Acids, vol. 16, no. 7-9, pp. 1439-1442 . https://doi.org/10.1080/07328319708006201
Scopus-Elsevier

The stereoselective synthesis of 3′-C-Allyluridine derivative 2 has been accomplished. This nucleoside was used as a key synthon for the synthesis of oligodeoxynucleotides containing 3′-C-(3-benzoyloxypropyl)thymidine (X) or bicyclo nucleoside (Y

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9dbea00608e47c08bce3486e8a5046a
https://doi.org/10.1080/07328319708006201

Zobrazit plný text záznamu

Synthesis and hybridization properties of β- and α-oligodeoxynucleotides containing β- and α-1-(3-C-allyl-2-deoxy-D-erythro-pentofuranosyl)thymine and α-1-[3-C-(3-aminopropyl)-2-deoxy-D-erythro-pentofuranosyl]thymine

Autor: Carl Erik Olsen, Ulrik S. Sørensen, Henrik M. Pfundheller, Jesper Wengel, Pia N. Jørgensen

Publikováno v: Journal of the Chemical Society, Perkin Transactions 1. :3275-3284

Convergent synthesis of β- and α-1-(3-C-allyl-2-deoxy-D-erythro-pentofuranosyl)thymine and their incorporation into β- and α-oligodeoxynucleotides (ODNs) is described. The thermal stabilities of duplexes formed between modified ODNs and complemen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2a59c5f8d6b9b02fdf75ca94ae831d3b
https://doi.org/10.1039/a703173d

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání