In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations.

Autor: Hansen, Henrik Frydenlund¹, Albaek, Nanna¹, Hansen, Bo Rode¹, Shim, Irene², Bohr, Henrik³ hbohr@dtu.dk, Koch, Troels¹

Publikováno v: Scientific Reports. 3/18/2021, Vol. 11 Issue 1, p1-13. 13p.

Identifying and avoiding off-target effects of RNase H-dependent antisense oligonucleotides in mice

Autor: Marco Berrera, Marianne Lerbech Jensen, Martin Ebeling, Peter Hagedorn, Marianne R. Møller, Morten Lindow, Troels Koch, Henrik Frydenlund Hansen, Malene Pontoppidan, Heidi Rye Hudlebusch, Andreas Dieckmann, Tina S Bisgaard

Publikováno v: Nucleic Acids Research
Hagedorn, P H, Pontoppidan, M, Bisgaard, T S, Berrera, M, Dieckmann, A, Ebeling, M, Møller, M R, Hudlebusch, H, Jensen, M L, Hansen, H F, Koch, T & Lindow, M 2018, ' Identifying and avoiding off-target effects of RNase H-dependent antisense oligonucleotides in mice ', Nucleic Acids Research, vol. 46, no. 11, pp. 5366-5380 . https://doi.org/10.1093/nar/gky397

Antisense oligonucleotides that are dependent on RNase H for cleavage and subsequent degradation of complementary RNA are being developed as therapeutics. Besides the intended RNA target, such oligonucleotides may also cause degradation of unintended

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5852a56ae13fabda0113b0fcc3dafeb6
https://doi.org/10.1093/nar/gky397

Electronic Structures of LNA Phosphorothioate Oligonucleotides

Autor: Troels Koch, Cy A. Stein, Henrik Ørum, Henrik Bohr, Henrik Frydenlund Hansen, Irene Shim

Publikováno v: Bohr, H G, Shim, I, Stein, C, Ørum, H, Hansen, H F & Koch, T 2017, ' Electronic Structures of LNA Phosphorothioate Oligonucleotides ', Molecular Therapy-Nucleic Acids, vol. 8, pp. 428-441 . https://doi.org/10.1016/j.omtn.2017.05.011
Molecular Therapy. Nucleic Acids
Molecular Therapy: Nucleic Acids, Vol 8, Iss, Pp 428-441 (2017)

Important oligonucleotides in anti-sense research have been investigated in silico and experimentally. This involves quantum mechanical (QM) calculations and chromatography experiments on locked nucleic acid (LNA) phosphorothioate (PS) oligonucleotid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9a79582c0a8bf77729d7d4a2d7cf227
https://doi.org/10.1016/j.omtn.2017.05.011

Locked nucleic acid: modality, diversity, and drug discovery

Autor: Henrik Frydenlund Hansen, Marianne R. Møller, Peter Hagedorn, Dennis Jul Hansen, Mads Jensen, Erik Funder, Nanna Albæk, Helle Christiansen, Natalia Papargyri, Sanna L. Diemer, Troels Koch, Robert Persson, Bo Hansen

Publikováno v: Hagedorn, P H, Persson, R, Funder, E D, Albæk, N, Diemer, S L, Hansen, D J, Møller, M R, Papargyri, N, Christiansen, H, Hansen, B R, Hansen, H F, Jensen, M A & Koch, T 2018, ' Locked nucleic acid: modality, diversity, and drug discovery ', Drug Discovery Today, vol. 23, no. 1, pp. 101-114 . https://doi.org/10.1016/j.drudis.2017.09.018

Over the past 20 years, the field of RNA-targeted therapeutics has advanced based on discoveries of modified oligonucleotide chemistries, and an ever-increasing understanding of how to apply cellular assays to identify oligonucleotides with improved

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e87e530133cecf9154e084346d5c3e2
https://orbit.dtu.dk/en/publications/b710204c-53b5-4d78-bbbb-8771e480792b

Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates

Autor: Niels Fisker, Ellen Marie Straarup, Henrik Ørum, Henrik Frydenlund Hansen, Christoph Rosenbohm, Jens Bo Hansen, Vibeke Aarup, Troels Koch, Marie Lindholm, Maj Hedtjärn

Publikováno v: Nucleic Acids Research

The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87022396d3372bd00e7c4e8fde7e622d
https://doi.org/10.1093/nar/gkq457