Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins

Autor: Joury S van 't Klooster, Tan-Yun Cheng, Hendrik R Sikkema, Aike Jeucken, Branch Moody, Bert Poolman

Publikováno v: eLife, Vol 9 (2020)

Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the questio

Externí odkaz: https://doaj.org/article/51119df5db0440f5a4a92bf00b7bc3e5

A synthetic metabolic network for physicochemical homeostasis

Autor: Tjeerd Pols, Hendrik R. Sikkema, Bauke F. Gaastra, Jacopo Frallicciardi, Wojciech M. Śmigiel, Shubham Singh, Bert Poolman

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)

Functional out-of-equilibrium networks are typical of living cells. Here the authors report the construction of a sustained ATP production system in vesicles with controlled energy dissipation and physicochemical homeostasis.

Externí odkaz: https://doaj.org/article/978bc8a91a0141beb7a7d957b10b5695

Heterodimer Formation of the Homodimeric ABC Transporter OpuA

Autor: Bert Poolman, Hendrik R Sikkema, Patricia Alvarez-Sieiro

Publikováno v: International Journal of Molecular Sciences
Volume 22
Issue 11
International Journal of Molecular Sciences, 22(11):5912. MDPI AG
International Journal of Molecular Sciences, Vol 22, Iss 5912, p 5912 (2021)

Many proteins have a multimeric structure and are composed of two or more identical subunits. While this can be advantageous for the host organism, it can be a challenge when targeting specific residues in biochemical analyses. In vitro splitting and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cf04f0822dfc3effb448eca672d6968
https://doi.org/10.3390/ijms22115912

Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA

Autor: Marijn de Boer, Gea K. Schuurman-Wolters, Bert Poolman, Cristina Paulino, Marco van den Noort, Jan Rheinberger, Hendrik R Sikkema, Sabrina T Krepel

Publikováno v: Science Advances, 6(47):eabd7697. AMER ASSOC ADVANCEMENT SCIENCE
Science Advances

Structural and biochemical studies reveal the dual regulation of the ABC transporter OpuA by ionic strength and cyclic-di-AMP.
(Micro)organisms are exposed to fluctuating environmental conditions, and adaptation to stress is essential for surviv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ed6ac502e49dd8c2f778f0b5087c7a2
https://research.rug.nl/en/publications/e8ad2110-44c5-48b8-aff5-5bb2f05cdf6e

Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins

Autor: Aike Jeucken, Hendrik R Sikkema, Bert Poolman, Branch Moody, Joury S van 't Klooster, Tan-Yun Cheng

Publikováno v: eLife, Vol 9 (2020)
eLife, 9:e57003. ELIFE SCIENCES PUBLICATIONS LTD

Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the questio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd82f0b8dd89238af41b7f674638102d