Optics miniaturization strategy for demanding Raman spectroscopy applications

Autor: Oleksii Ilchenko, Yurii Pilhun, Andrii Kutsyk, Denys Slobodianiuk, Yaman Goksel, Elodie Dumont, Lukas Vaut, Chiara Mazzoni, Lidia Morelli, Sofus Boisen, Konstantinos Stergiou, Yaroslav Aulin, Tomas Rindzevicius, Thomas Emil Andersen, Mikael Lassen, Hemanshu Mundhada, Christian Bille Jendresen, Peter Alshede Philipsen, Merete Hædersdal, Anja Boisen

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)

Abstract Raman spectroscopy provides non-destructive, label-free quantitative studies of chemical compositions at the microscale as used on NASA’s Perseverance rover on Mars. Such capabilities come at the cost of high requirements for instrumentati

Externí odkaz: https://doaj.org/article/def42b92f67c4f4abe9029fe344c3bff

P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length

Autor: Ketaki D. Belsare, Anna Joëlle Ruff, Ronny Martinez, Amol V. Shivange, Hemanshu Mundhada, Dirk Holtmann, Jens Schrader, Ulrich Schwaneberg

Publikováno v: BioTechniques, Vol 57, Iss 1, Pp 13-20 (2014)

Fusion protein construction is a widely employed biochemical technique, especially when it comes to multi-component enzymes such as cytochrome P450s. Here we describe a novel method for generating fusion proteins with variable linker lengths, protein

Externí odkaz: https://doaj.org/article/4edd6cc837344704b4a638b5902833ca

Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Autor: Adam M. Feist, Isaac Cann, Hemanshu Mundhada, Markus J. Herrgård, Elsayed Tharwat Tolba Mohamed, Roderick I. Mackie, Jenny Marie Landberg, Alex Toftgaard Nielsen

Publikováno v: Microbial Cell Factories, Vol 18, Iss 1, Pp 1-14 (2019)
Mohamed, E T T, Mundhada, H, Landberg, J M, Cann, I, Mackie, R I, Nielsen, A T, Herrgard, M J & Feist, A M 2019, ' Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution ', Microbial Cell Factories, vol. 18, 116 . https://doi.org/10.1186/s12934-019-1165-2
Microbial Cell Factories

Background Sucrose is an attractive industrial carbon source due to its abundance and the fact that it can be cheaply generated from sources such as sugarcane. However, only a few characterized Escherichia coli strains are able to metabolize sucrose,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f95422196f87e6708be8911c79677f85
http://link.springer.com/article/10.1186/s12934-019-1165-2

Industrializing a Bacterial Strain for l -Serine Production through Translation Initiation Optimization

Autor: Gossa Garedew Wordofa, Andreas Worberg, Maja Rennig, Hemanshu Mundhada, Tune Wulff, Daniel Gerngross, Alex Toftgaard Nielsen, Morten H. H. Nørholm

Publikováno v: Rennig, M, Mundhada, H, Wordofa, G G, Gerngross, D, Wulff, T, Worberg, A, Nielsen, A T & Nørholm, M H H 2019, ' Industrializing a Bacterial Strain for l-Serine Production through Translation Initiation Optimization ', ACS Synthetic Biology, vol. 8, no. 10, pp. 2347-2358 . https://doi.org/10.1021/acssynbio.9b00169

Turning a proof-of-concept synthetic biology design into a robust, high performing cell factory is a major time and money consuming task, which severely limits the growth of the white biotechnology sector. Here, we extend the use of tunable antibioti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::367980338c5e22ca484e713fe03c0522
https://orbit.dtu.dk/en/publications/1cfd6370-cabc-4061-bd2b-36969905f29b

Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides

Autor: Songyuan Li, Christian Bille Jendresen, Lasse Ebdrup Pedersen, Jenny Landberg, Kristoffer Bach Falkenberg, Hemanshu Mundhada, Alex Toftgaard Nielsen

Publikováno v: Li, S, Jendresen, C B, Pedersen, L E, Landberg, J M, Falkenberg, K B, Mundhada, H & Nielsen, A T, Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides, Patent No. WO2018020012 .
Lasse Ebdrup Pedersen
Technical University of Denmark Orbit
The Lens

The present invention generally relates to industrial microbiology, and specifically to the production of biochemical compounds, such as L-serine, L-tyrosine, mevalonate and their derivatives, and recombinant polypeptides using genetically modified m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::4403dd0b73fd6d91ceef13d58f20151a
https://orbit.dtu.dk/en/publications/20a26a76-9401-4cda-8889-2ae6b08f7d5d