Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Helmuth Heithier"'
Autor:
Winchil L.C. Vaz, Mirko Hekman, Helmuth Heithier, Martin Baumann, Ernst J.M. Helmreich, Margareta Frohlich, Martin Häring, Christian Dees, Emile Schiltz, Peter Gierschik
Publikováno v:
European Journal of Biochemistry. 204:1169-1181
Fluorescence energy transfer [cf. Förster, T. (1948) Ann. Phys. 6, 55-75] was tested for its suitability to study quantitative interactions of subunits of G0 with each other and these subunits or trimeric G0 with the beta 1-adrenoceptor in detergent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4be600b8e8d752098448297f92304036
https://doi.org/10.1111/j.1432-1033.1992.tb16744.x
https://doi.org/10.1111/j.1432-1033.1992.tb16744.x
Publikováno v:
Biochemical and Biophysical Research Communications. 167:1162-1169
Glucagon and prostaglandin E 1 stimulate adenylate cyclase in Madin-Darby canine kidney cells with an approximate EC 50 of 3 ∗ 10 −8 and 1 ∗ 10 −7 M respectively. The rise in cAMP is accompanied by a transient rise in intracellular Ca ++ meas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c1e8227940594885ec2292a744b0a0
https://doi.org/10.1016/0006-291x(90)90645-4
https://doi.org/10.1016/0006-291x(90)90645-4
Autor:
Helmut W. Klein, Richard C. Cantrill, Reiner Peters, Helmuth Heithier, Larry W. Ward, Ernst J.M. Helmreich
Publikováno v:
Berichte der Bunsengesellschaft für physikalische Chemie. 92:973-978
New fluorescent glucagon derivatives were synthesized by converting tryptophan25 to 2-thiol-tryptophan with the consequent use of thiol specific fluorescent reagents. All derivatives retained the ability to bind tightly to rat liver membranes and rat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1c46526d490e359719ea5b4eaad7c622
https://doi.org/10.1002/bbpc.198800243
https://doi.org/10.1002/bbpc.198800243
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 971:307-316
When monolayer cultured hepatocytes were incubated with 1 nM [125I]glucagon at 30 degrees C, equilibrium was reached after 10 min, whereas at 4 degrees C, equilibrium was reached after 60 min. At the higher temperature, 11.2% of the bound ligand was
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::960b005eb94c23a43aa55883543f562e
https://doi.org/10.1016/0167-4889(88)90146-2
https://doi.org/10.1016/0167-4889(88)90146-2
Autor:
Mirko Hekman, Dieter Palm, Martin Baumann, Helmuth Heithier, M. Köstler, Ernst J.M. Helmreich, Gerald Münch
Publikováno v:
Journal of protein chemistry. 8(3)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e5dd870153d20ded7f7344ca89bc089
https://pubmed.ncbi.nlm.nih.gov/2551324
https://pubmed.ncbi.nlm.nih.gov/2551324
Publikováno v:
Biochimie. 70(5)
The synthesis and properties of a fluorescent derivative of the hydrophilic beta-adrenergic antagonist CGP-12177 are described. The fluorescence of the NBD derivative of CGP-12177 (CGP-NBD) is extremely sensitive to its environment, the quantum yield
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a70c019f33a1cc928d9dc355b2f8aa9
https://pubmed.ncbi.nlm.nih.gov/2901863
https://pubmed.ncbi.nlm.nih.gov/2901863
Autor:
Richard C. Cantrill, Ernst J.M. Helmreich, Helmut W. Klein, Mi-Jae Im, Gabriele Pollak, Barbara Freeman, Helmuth Heithier, Reiner Peters, Emile Schiltz, Larry D. Ward
Publikováno v:
Biochimica et biophysica acta. 971(3)
The synthesis of monofluorescein, monorhodamine, and mono-4-nitrobenz-2-oxa-1,3-diazole (NBD) derivatives of glucagon is reported. The fluorescent groups were introduced by converting tryptophan-25 to 2-thioltryptophan using thiol-specific fluorescen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4697062afdac8a2f62710a1bb1458fed
https://pubmed.ncbi.nlm.nih.gov/2844291
https://pubmed.ncbi.nlm.nih.gov/2844291