Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Helmut Merk"'
Publikováno v:
BioTechniques, Vol 53, Iss 3, Pp 153-160 (2012)
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins. The system uses (i) a cell lysate that is mildly prepared under sl
Externí odkaz:
https://doaj.org/article/f4c04bb21c2f4d5c9b990bf3c142106b
Autor:
Paul Majkut, Iris Claußnitzer, Helmut Merk, Christian Freund, Christian P R Hackenberger, Michael Gerrits
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e82352 (2013)
The characterization of phosphotyrosine mediated protein-protein interactions is vital for the interpretation of downstream pathways of transmembrane signaling processes. Currently however, there is a gap between the initial identification and charac
Externí odkaz:
https://doaj.org/article/1f37255e948f4634ba364d2df5f58478
Publikováno v:
ACS Synthetic Biology. 8:381-390
Cell-free protein synthesis (CFPS) is a fast and convenient way to synthesize proteins for analytical studies and applications. CFPS, when equipped with a suitable orthogonal pair, allows for protein-site-directed labeling with desired functionalitie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1318ebbe2ac9021ac9ba6444ae21073f
https://doi.org/10.1021/acssynbio.8b00421
https://doi.org/10.1021/acssynbio.8b00421
Autor:
Helmut Merk, Frank F. Bier, Stefan Kubick, Burkhard Micheel, Jörg A. Schenk, Walter Stöcklein, Claus Duschl, Marlitt Stech, Doreen A. Wüstenhagen
Publikováno v:
Journal of Biotechnology. 164:220-231
Cell-free protein synthesis is of increasing interest for the rapid and high-throughput synthesis of many proteins, in particular also antibody fragments. In this study, we present a novel strategy for the production of single chain antibody fragment
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74847eb265c72ea5e209e0e5dcc57e5a
https://doi.org/10.1016/j.jbiotec.2012.08.020
https://doi.org/10.1016/j.jbiotec.2012.08.020
Autor:
Christine Gless, Michael Gerrits, Helmut Merk, Kerstin Beyer, Fang Dong, Frank Bernhard, Volker Dötsch, Ralf-Bernhardt Rues
Publikováno v:
Biological chemistry. 396(9-10)
G protein-coupled receptors, like many other membrane proteins, are notoriously difficult to synthesize in conventional cellular systems. Although expression in insect cells is considered the preferred technique for structural characterizations in pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::662fbfafd5c6abd97c7f903580a76807
https://pubmed.ncbi.nlm.nih.gov/25999328
https://pubmed.ncbi.nlm.nih.gov/25999328
Eukaryotic cell-free systems based on wheat germ and Spodoptera frugiperda insect cells were equipped with an orthogonal amber suppressor tRNA–synthetase pair to synthesize proteins with a site-specifically incorporated p -azido- l -phenylalanine r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf4d38c0aef8ab6f99d5e088da73991d
https://publica.fraunhofer.de/handle/publica/237269
https://publica.fraunhofer.de/handle/publica/237269
Publikováno v:
Journal of Biochemistry. 125:328-333
Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the revers
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a554d6c8db0d69d0cd944e458494cc1b
https://doi.org/10.1093/oxfordjournals.jbchem.a022290
https://doi.org/10.1093/oxfordjournals.jbchem.a022290
Publikováno v:
BioTechniques. 53(3)
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins. The system uses (i) a cell lysate that is mildly prepared under sl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b29f99384ae6e4db41c87258c9eedee
https://pubmed.ncbi.nlm.nih.gov/22963477
https://pubmed.ncbi.nlm.nih.gov/22963477
Publisher Summary Membrane proteins have become an important focus of the current efforts in structural and functional genomics and the rapid progress of various genome sequencing projects has greatly accelerated the discovery of novel genes encoding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::99356e75dead8abc50a28ddfb0bd170a
https://doi.org/10.1016/s1063-5823(09)63002-7
https://doi.org/10.1016/s1063-5823(09)63002-7
Autor:
Jan Strey, Michael Gerrits, Wolfgang Stiege, Christine Gless, Stefan Kubick, Doreen Kazmierczak, Katrin Ahrens, Helmut Merk, Jens Schacherl
Publikováno v:
GBM Annual Fall meeting Berlin/Potsdam 2005. 2005
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ec8aca21c4fe6c0b54b802dbc33012e8
https://doi.org/10.1240/sav_gbm_2005_h_001271
https://doi.org/10.1240/sav_gbm_2005_h_001271