Výsledky vyhledávání - "Helmut Gnaegi"

Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution

Autor: Helmut Gnaegi, Georg Kislinger, Mikael Simons, Martin Kerschensteiner, Thomas Misgeld, Martina Schifferer

Publikováno v: iScience, Vol 23, Iss 7, Pp 101290-(2020)
iScience

Summary Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04c97030ba2cc45fad9ff393def3a387
https://doi.org/10.1017/s1431927620015238

Zobrazit plný text záznamu

Development of protocols for the first serial block-face scanning electron microscopy (SBF SEM) studies of bone tissue

Autor: Helmut Gnaegi, Elaine M.L. Ho, Philipp Schneider, Richard O.C. Oreffo, Stuart Searle, Patricia Goggin

Publikováno v: Bone

Graphical abstract
Highlights • Introduced SBF SEM for hard tissues, beyond current limitation to soft tissues only. • Realised concurrent high-resolution 3D imaging of soft & hard bone tissue components. • Imaged the osteocyte and lacuno-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c7623b08828333c4f2ed48e4ad4d84f
http://europepmc.org/articles/PMC6961117

Zobrazit plný text záznamu

Comparison of ultramicrotomy and focused-ion-beam for the preparation of TEM and STEM cross section of organic solar cells

Autor: Karl Tor Sune Thydén, Frederik C. Krebs, Søren Bredmose Simonsen, Helmut Gnaegi, Michael Corazza, Suren A. Gevorgyan

Publikováno v: Applied Surface Science. 389:462-468

The challenge of preparing cross sections of organic photovoltaics (OPV) suitable for transmission electron microscopy (TEM) and scanning TEM (STEM) is addressed. The samples were polymer solar cells fabricated using roll-to-roll (R2R) processing met

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8ab72d48007309b54b61b5b328b3c6a0
https://doi.org/10.1016/j.apsusc.2016.07.096

Zobrazit plný text záznamu

A targeted 3D EM and correlative microscopy method using SEM array tomography

Autor: Helmut Gnaegi, Clément Chevalier, Agnès Burel, Steṕhanie Dutertre, Irina Kolotuev, Sylvain Prigent, Antonio Mucciolo, Thierry Guillaudeux, Marie Theŕèse Lavault, Bruno M. Humbel

Publikováno v: Development
Development, 2018, 〈10.1242/dev.160879〉
Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2018, 145 (12), pp.dev160879. ⟨10.1242/dev.160879⟩
Development (Cambridge, England), 2018, 145 (12), pp.dev160879. ⟨10.1242/dev.160879⟩

International audience; Using electron microscopy to localize rare cellular events or structures in complex tissue is challenging. Correlative light and electron microscopy procedures have been developed to link fluorescent protein expression with ul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b86dbb45a6a3f733db148dfda1880aee
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01808769

Zobrazit plný text záznamu

Coating of Alumina Fibres With Aluminium Phosphate by a Continuous Chemical Vapour Deposition Process

Autor: Steffen Schulze, Helmut Gnaegi, Thomas Wamser, Michael Hietschold, Sabine Stöckel, Susann Ebert, Andreas Seifert, Maike Böttcher, Walter Krenkel, Werner A. Goedel

Publikováno v: Chemical Vapor Deposition. 20:388-398

We developed aluminium phosphate-coated alumina fibres with significantly improved pull-out properties, which may be suitable for reinforcing advanced inorganic composites qualified for operating temperatures up to 1200 °C. Aluminium phosphate layer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d421846e101c5063b4a5ded917c83275
https://doi.org/10.1002/cvde.201407099

Zobrazit plný text záznamu

Micromachining tools and correlative approaches for cellular cryo-electron tomography

Autor: Tim Laugks, Jürgen M. Plitzko, Matthias Eibauer, Alexander Rigort, Felix J.B. Bäuerlein, Manuela Gruska, Wolfgang Baumeister, Andrew Leis, Helmut Gnaegi, Christian Hoffmann, Ulrike Böhm

Publikováno v: Journal of Structural Biology; Vol 172
Journal of Structural Biology

A principal limitation of cryo-transmission electron microscopy performed on cells or tissues is the accessible specimen thickness. This is exacerbated in tomography applications, where the aspect ratio (and thus the apparent specimen thickness) chan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9278e21c9a4e139feaced0e9800f1ecb

Zobrazit plný text záznamu

Preservation of high resolution protein structure by cryo-electron microscopy of vitreous sections

Autor: Helmut Gnaegi, Benoît Zuber, K Sader, John Trinick, Daniel Studer

Publikováno v: Ultramicroscopy

We have quantitated the degree of structural preservation in cryo-sections of a vitrified biological specimen. Previous studies have used sections of periodic specimens to assess the resolution present, but preservation before sectioning was not asse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::748904a6493385a59762fba5e07965ec
https://doi.org/10.1016/j.ultramic.2009.09.004

Zobrazit plný text záznamu

Characterization of the cutting edge of glass and diamond knives for ultramicrotomy by scanning force microscopy using cantilevers with a defined tip geometry. Part II

Autor: Rudolf Reichelt, Andrea Ricker, Thomas Matzelle, Helmut Gnaegi

Publikováno v: Journal of Microscopy. 209:113-117

The cutting edge of glass as well as diamond knives was studied at high resolution using a scanning force microscope (SFM). The local shape of the cutting edge was estimated from single line profiles of the SFM topographs taking into account the exac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c381ecc623b47c6d2146404da287bca4
https://doi.org/10.1046/j.1365-2818.2003.01119.x

Zobrazit plný text záznamu

A new tool based on two micromanipulators facilitates the handling of ultrathin cryosection ribbons

Autor: Daniel Studer, Benoît Zuber, Ioan Iacovache, Helmut Gnaegi, Alycia Klein

Publikováno v: Studer, Daniel Franz; Klein, Alycia; Iacovache, Mircea Ioan; Gnaegi, Helmut; Zuber, Benoît (2014). A new tool based on two micromanipulators facilitates the handling of ultrathin cryosection ribbons. Journal of structural biology, 185(1), pp. 125-128. Elsevier 10.1016/j.jsb.2013.11.005
Journal of structural biology

A close to native structure of bulk biological specimens can be imaged by cryo-electron microscopy of vitreous sections (CEMOVIS). In some cases structural information can be combined with X-ray data leading to atomic resolution in situ. However, CEM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cc736baf43323e478b3718a7d130571
https://boris.unibe.ch/46869/1/Studer_Micromanipulators_2014.pdf

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání