A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry

Autor: Samuel Wein, Byron Andrews, Timo Sachsenberg, Helena Santos-Rosa, Oliver Kohlbacher, Tony Kouzarides, Benjamin A. Garcia, Hendrik Weisser

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)

Mass spectrometry (MS) enables identification of modified RNA residues, but high-throughput processing is currently a bottleneck. Here, the authors present a free and open-source database search engine for RNA MS data to facilitate reliable identific

Externí odkaz: https://doaj.org/article/3d1187b1870042518abd5163793bbe16

RNA Binding by Histone Methyltransferases Set1 and Set2

Autor: Helena Santos-Rosa, Samuel Robson, Gonzalo Millán-Zambrano, Elisabeth Petfalski, David Tollervey, Camille Sayou, Jonathan Houseley, Tony Kouzarides

Publikováno v: Molecular and Cellular Biology
Sayou, C, Millan-Zambrano, G, Santos-Rosa, H, Petfalski, E, Robson, S, Houseley, J, Kouzarides, T & Tollervey, D 2017, ' RNA binding by the histone methyltransferases Set1 and Set2 ', Molecular and Cellular Biology, vol. 37, no. 14, e00165-17 . https://doi.org/10.1128/MCB.00165-17

Histone methylation at H3K4 and H3K36 is commonly associated with genes actively transcribed by RNA polymerase II (RNAPII) and is catalyzed by Saccharomyces cerevisiae Set1 and Set2, respectively. Here we report that both methyltransferases can be UV

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e98bb2551938e87c4fad50ce25fd772
http://europepmc.org/articles/PMC5492175

The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth

Autor: Helena Santos-Rosa, Symeon Siniossoglou, Joanne Leung, Neil Grimsey, Sew Y. Peak-Chew

Publikováno v: The EMBO Journal. 24:1931-1941

Remodelling of the nuclear membrane is essential for the dynamic changes of nuclear architecture at different stages of the cell cycle and during cell differentiation. The molecular mechanism underlying the regulation of nuclear membrane biogenesis i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc55cd99a0912c6b5e6ae31304c3cf3e
https://doi.org/10.1038/sj.emboj.7600672

Active genes are tri-methylated at K4 of histone H3

Autor: Julia A. Sherriff, Jane Mellor, Andrew J. Bannister, N. C. Tolga Emre, Bradley E. Bernstein, Robert Schneider, Helena Santos-Rosa, Stuart L. Schreiber, Tony Kouzarides

Publikováno v: Nature. 419:407-411

Lysine methylation of histones in vivo occurs in three states: mono-, di- and tri-methyl. Histone H3 has been found to be di-methylated at lysine 4 (K4) in active euchromatic regions but not in silent heterochromatic sites. Here we show that the Sacc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::134dbb925cf775ddc63c65bb9eea517e
https://doi.org/10.1038/nature01080

Proline isomerization of histone H3 regulates lysine methylation and gene expression

Autor: Christopher J. Nelson, Helena Santos-Rosa, Tony Kouzarides

Publikováno v: Cell. 126(5)

SummaryThe cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::112e7c086ff24e4fd459a8ccd15e8ebe
https://pubmed.ncbi.nlm.nih.gov/16959570