Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR

Autor: Gilbert O. Silveira, Murilo S. Amaral, Helena S. Coelho, Lucas F. Maciel, Adriana S. A. Pereira, Giovanna G. O. Olberg, Patricia A. Miyasato, Eliana Nakano, Sergio Verjovski-Almeida

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)

Abstract Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most used, fast, and reproducible method to confirm large-scale gene expression data. The use of stable reference genes for the normalization of RT-qPCR

Externí odkaz: https://doaj.org/article/e3e22ccbe69e4880829662ea5ea2957f

Long non-coding RNAs as possible therapeutic targets in protozoa, and in Schistosoma and other helminths

Autor: Sergio Verjovski-Almeida, Gilbert O Silveira, Murilo S. Amaral, Helena S. Coelho

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Long non-coding RNAs (lncRNAs) emerged in the past 20 years due to massive amounts of scientific data regarding transcriptomic analyses. They have been implicated in a plethora of cellular processes in higher eukaryotes. However, little is known abou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::024a11e5340a809fae6fc04d80d04add
https://doi.org/10.1007/s00436-021-07384-5

Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR

Autor: Lucas F. Maciel, Giovanna G. O. Olberg, Eliana Nakano, Helena S. Coelho, Patricia A. Miyasato, Sergio Verjovski-Almeida, Gilbert O. Silveira, Murilo S. Amaral, Adriana S. A. Pereira

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
Scientific Reports

Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most used, fast, and reproducible method to confirm large-scale gene expression data. The use of stable reference genes for the normalization of RT-qPCR assays is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34ce8e419c068835e10690c154beb4f1

A phytochemical study of the Cuphea glutinosa from Southern Brazil: Na+,K+-ATPase activity inhibition and antioxidant properties

Autor: Jessié Martins Gutierres, Fabiano B. Carvalho, Ademir F. Morel, Cinthia Melazzo de Andrade, Marilda da Cruz Fernandes, Aline de Oliveira Fogaça, Liziane Maria Barassuol Morandini, Ionara I. Dalcol, Helena S. Coelho, Adriana Maria Zago, Marco A. Mostardeiro, Crystiani Bohnert

This study investigated the antioxidant activity of Cuphea glutinosa (CG) and its effect on Na+, K+-ATPase from cardiac muscle. The ethanolic extract showed higher antioxidant capacity compared to aqueous and ethyl acetate fraction. Ethyl acetate fra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2222a26a898ab1902aff6c7983a6ea7c

Synthesis and characterization of new trifluoromethyl substituted 3‐ethoxycarbonyl‐ and 3‐pyrimidin‐2‐yl)‐(1,2,3)‐oxathiazinane‐S‐oxides

Autor: Deise M. Borchhardt, Nilo Zanatta, Tiago M. Marchi, Darlene C. Flores, Helena S. Coelho, Alex F. C. Flores, Helio G. Bonacorso, Marcos A. P. Martins

Publikováno v: Journal of Heterocyclic Chemistry. 45:335-341

This paper describes the synthesis and characterization of a new series of 4-substituted-3-ethoxycarbonyl-6-trifluoromethyl-(1,2,3)-oxathiazinane-S-oxides and 3-(4,6-diphenyl-pyrimidin-2-yl)-6-trifluoromethyl-(1,2,3)oxathiazinane-S-oxides from the cy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f32e3901cce12033b6978ddfeda0848a
https://doi.org/10.1002/jhet.5570450207