Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Helena M. Scofano"'
Autor:
Rosa dos Prazeres Melo Furriel, D. C. Masui, Fernando Luis Medina Mantelatto, Helena M. Scofano, E. C. C. Silva, John Campbell McNamara, Hector Barrabin, Carlos Frederico Leite Fontes, Francisco de Assis Leone
Publikováno v:
Archives of Biochemistry and Biophysics. 479:139-144
The blue crab, Callinectes danae, tolerates exposure to a wide salinity range employing mechanisms of compensatory ion uptake when in dilute media. Although the gill (Na+,K+)-ATPase is vital to hyperosmoregulatory ability, the interactions occurring
Autor:
Francisco de Assis Leone, Fernando Luis Medina Mantelatto, John Campbell McNamara, Rosa dos Prazeres Melo Furriel, Helena M. Scofano, Hector Barrabin, E. C. C. Silva, Carlos Frederico Leite Fontes, D. C. Masui
Publikováno v:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 149:622-629
Euryhaline crustaceans rarely hyporegulates and employ the driving force of the Na,K-ATPase, located at the basal surface of the gill epithelium, to maintain their hemolymph osmolality within a range compatible with cell function during hyper-regulat
Autor:
Helena M. Scofano, Patricia Souza dos Santos, Dayvison Francis Saraiva, Danielly C. Ferraz da Costa, Paulo C. Carvalho-Alves
Publikováno v:
Experimental Brain Research. 177:347-357
In the central nervous system (CNS), a number of different pathological processes such as necrosis, Parkinson's and Alzheimer's diseases are related to disturbance in calcium homeostasis associated with oxidative stress. Here we compare the susceptib
Autor:
Hector Barrabin, Helena M. Scofano, Joyce R. Pearlstone, Monica M. Freire, Elizabeth Fidalgo da Silva, J. David Johnson, Svetlana B. Tikunova, Murali Chandra, Martha M. Sorenson
Publikováno v:
The International Journal of Biochemistry & Cell Biology. 38:209-221
Calmodulin (CaM) and troponin C (TnC) are EF-hand proteins that play fundamentally different roles in animal physiology. TnC has a very low affinity for the plasma membrane Ca2+-ATPase and is a poor substitute for CaM in increasing the enzyme's affin
Autor:
J.R. Meyer-Fernandes, Helena M. Scofano, Angela H. Lopes, M. Alves-Ferreira, Antonio Ferreira-Pereira, P. M. L. Dutra
Publikováno v:
Current Microbiology. 47:265-271
In the present work we characterized the ecto-ATP diphosphohydrolase activity of the trypanosomatid parasite Herpetomonas muscarum muscarum. This parasite hydrolyzed ATP at a rate of 15.52 nmol Pi/mg protein/min and this activity reached a maximum at
Publikováno v:
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 131:405-415
Kinetic and regulatory properties of the plasma membrane Ca 2+ -ATPase activity from chicken (nucleated) erythrocytes were studied and compared to those from pig (anucleated) erythrocytes. In the absence of known activators: (1) Ca 2+ affinity for th
Autor:
Hector Barrabin, Paulo C. Carvalho-Alves, Julio A. Mignaco, Helena M. Scofano, Monica M. Freire
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1553:238-248
3-O-Methylfluorescein phosphate hydrolysis, catalyzed by purified erythrocyte Ca2+-ATPase in the absence of Ca2+, was slow in the basal state, activated by phosphatidylserine and controlled proteolysis, but not by calmodulin. p-Nitrophenyl phosphate
Publikováno v:
Archives of Biochemistry and Biophysics. 376:141-148
Soluble purified CF(0)F(1) from chloroplasts was either oxidized or reduced and then incubated with [alpha-(32)P]ATP in the presence or in the absence of Mg(2+). Depending on the conditions of incubation, the enzyme showed different tight-nucleotide
Autor:
Carlos Frederico Leite Fontes, Fábio E.Veiga Lopes, Helena M. Scofano, Hector Barrabin, Jens G. Nørby
Publikováno v:
Archives of Biochemistry and Biophysics. 366:215-223
In 40% dimethyl sulfoxide (Me2SO) high-affinity ouabain (O) binding to Na,K-ATPase (E) is promoted by Mg2+ in the absence of inorganic phosphate (Pi) (Fontes et al., Biochim. Biophys. Acta 1104, 215–225, 1995). Furthermore, in Me2SO the EO complex
Publikováno v:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 122:269-276
In the present report we describe a method for purifying plasma membranes from chicken erythrocytes using sonication under conditions that facilitate preferential lysis of plasma membrane, followed by centrifugation through a sucrose gradient. The Ca