Structural insights into the function of type VI secretion system TssA subunits

Autor: Samuel R. Dix, Hayley J. Owen, Ruyue Sun, Asma Ahmad, Sravanthi Shastri, Helena L. Spiewak, Daniel J. Mosby, Matthew J. Harris, Sarah L. Batters, Thomas A. Brooker, Svetomir B. Tzokov, Svetlana E. Sedelnikova, Patrick J. Baker, Per A. Bullough, David W. Rice, Mark S. Thomas

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-16 (2018)

TssA is an important component of the bacterial type VI secretion system (T6SS). Here, Dix et al. integrate structural, phylogenetic and functional analysis of the TssA subunits, providing new insights into their role in T6SS assembly and function.

Externí odkaz: https://doaj.org/article/db75b9f94174499d95638950af73066d

Sequencing‐based microsatellite instability testing using as few as six markers for high‐throughput clinical diagnostics

Autor: Ciaron McAnulty, Ghanim Alhilal, Michael S. Jackson, Richard Gallon, Harsh Sheth, Sira Moreno Laguna, Gillian M. Borthwick, Angel Alonso, John Burn, Mauro Santibanez-Koref, Amanda Waltham, C Hayes, Helena L. Spiewak, Lisa Redford, Osagie G. Izuogu, Mark J. Arends, O. O'brien, Anca Oniscu

Publikováno v: Human Mutation
Gallon, R, Sheth, H, Hayes, C, Redford, L, Alhilal, G, O’Brien, O, Spiewak, H, Waltham, A, McAnulty, C, Izuogu, O, Arends, M, Oniscu, A, Miguel Alonso, Á, Moreno Laguna, S, Borthwick, G, Santibanez Koref, M, Jackson, M S & Burn, J 2019, ' Sequencing-based microsatellite instability testing using as few as six markers for high throughput clinical diagnostics ', Human Mutation . https://doi.org/10.1002/humu.23906

Microsatellite instability (MSI) testing of colorectal cancers (CRCs) is used to screen for Lynch syndrome (LS), a hereditary cancer‐predisposition, and can be used to predict response to immunotherapy. Here, we present a single‐molecule molecula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcf7107f845710b3b720be0ba8b2b221
https://doi.org/10.1002/humu.23906

An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia

Autor: Mark S. Thomas, Vigdis A. Eidsvaag, Sravanthi Shastri, Aaron T. Butt, Aderonke Sofoluwe, Atif H. Asghar, Helena L. Spiewak, Tyrone Pereira, Edward H. Bull

Publikováno v: Plasmid

To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::129022b42a9fafddf043b0d18a83c1f7