Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Helen K. Leech"'
Autor:
Hannah F Collins, Rebekka Biedendieck, Helen K Leech, Michael Gray, Jorge C Escalante-Semerena, Kirsty J McLean, Andrew W Munro, Stephen E J Rigby, Martin J Warren, Andrew D Lawrence
Publikováno v:
PLoS ONE, Vol 8, Iss 2, p e55708 (2013)
Despite the extensive study of the biosynthesis of the complex molecule B12 (cobalamin), the mechanism by which the lower ligand 5,6-dimethylbenzimidazole (DMB) is formed has remained something of a mystery. However, recent work has identified and ch
Externí odkaz:
https://doaj.org/article/bfd765d7e8924914a5f57c87c6186900
Autor:
Marco Malten, Boyke Bunk, Evelyne Deery, Jan-Henning Martens, Helen K. Leech, Martin J. Warren, Dieter Jahn, Rebekka Biedendieck, Heiko Barg
Publikováno v:
Microbial Biotechnology. 3:24-37
Cobalamin (vitamin B(12)) production in Bacillus megaterium has served as a model system for the systematic evaluation of single and multiple directed molecular and genetic optimization strategies. Plasmid and genome-based overexpression of genes inv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9a3cece77e0e213ee85e11e6ba85994b
https://doi.org/10.1111/j.1751-7915.2009.00125.x
https://doi.org/10.1111/j.1751-7915.2009.00125.x
Autor:
Martin J. Warren, Helen K. Leech, Susana A.L. Lobo, Amanda A. Brindley, Célia V. Romão, Lígia M. Saraiva
Publikováno v:
Biochemistry. 47:5851-5857
The sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough possesses a large number of porphyrin-containing proteins whose biosynthesis is poorly characterized. In this work, we have studied two putative CbiK cobaltochelatases present in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::450949a28c3dcc64bcbd05391a59d12d
https://doi.org/10.1021/bi800342c
https://doi.org/10.1021/bi800342c
Autor:
Stefanie Frank, Andy Lawrence, Stephen E. J. Rigby, Peter Heathcote, Helen K. Leech, Evelyne Deery, Heidi L. Schubert, Keith Brocklehurst, Martin J. Warren, Amanda A. Brindley, Richard W. Pickersgill
Publikováno v:
Journal of Biological Chemistry. 282:23957-23969
Ring contraction during cobalamin (vitamin B12) biosynthesis requires a seemingly futile methylation of the C20 position of the tetrapyrrole framework. Along the anaerobic route, this reaction is catalyzed by CbiL, which transfers a methyl group from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6716825abdb8da6086d0cd763a1256d3
https://doi.org/10.1074/jbc.m703827200
https://doi.org/10.1074/jbc.m703827200
Autor:
Gunhild Layer, Lothar Jänsch, Daniela Breckau, Stephen E. J. Rigby, Helen K. Leech, Dirk W. Heinz, Martin J. Warren, Dieter Jahn, Antonio J. Pierik, I. Astner, Katrin Grage, Matthias Trost, Peter Heathcote
Publikováno v:
Journal of Biological Chemistry. 281:15727-15734
During porphyrin biosynthesis the oxygen-independent coproporphyrinogen III oxidase (HemN) catalyzes the oxidative decarboxylation of the propionate side chains of rings A and B of coproporphyrinogen III to form protoporphyrinogen IX. The enzyme util
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::119319f73654168a694636b0b48be0b2
https://doi.org/10.1074/jbc.m512628200
https://doi.org/10.1074/jbc.m512628200
Autor:
Helen K. Leech, Richard W. Pickersgill, Peter Heathcote, Andy Lawrence, Evelyne Deery, Martin J. Warren, Stefanie Frank, Amanda A. Brindley
Publikováno v:
Biochemical Society Transactions. 33:811-814
The anaerobic biosynthesis of vitamin B12 is slowly being unravelled. Recent work has shown that the first committed step along the anaerobic route involves the sirohydrochlorin (chelation of cobalt into factor II). The following enzyme in the pathwa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbcd20761889a9926c26db9ef7fc07bb
https://doi.org/10.1042/bst0330811
https://doi.org/10.1042/bst0330811
Autor:
Kerry Ann Nakrieko, Andrew W. Munro, Stephen E. J. Rigby, Martin J. Warren, Evelyne Raux-Deery, Helen K. Leech, Alison G. Smith, Peter Heathcote, Kirsty J. McLean
Publikováno v:
Journal of Biological Chemistry. 280:4713-4721
Higher plant sulfite and nitrite reductases contain siroheme as a prosthetic group. Siroheme is synthesized from the tetrapyrrole primogenitor uroporphyrinogen III in three steps involving methylation, oxidation, and ferrochelation reactions. In this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c66ce7ef24f0fdd394e4becce4509822
https://doi.org/10.1074/jbc.m411360200
https://doi.org/10.1074/jbc.m411360200
Autor:
Gilles P.M. Borrelly, Stephen E. J. Rigby, Helen K. Leech, Peter Heathcote, Evelyne Raux, Kirsty J. McLean, Dieter Jahn, Martin J. Warren, Nigel J. Robinson, Andrew W. Munro, Marco Malten
Publikováno v:
Journal of Biological Chemistry. 278:41900-41907
CbiX is a cobaltochelatase required for the biosynthesis of vitamin B12 and is found in Archaea as a short form (CbiXS containing 120-145 amino acids) and in some bacteria as a longer version (CbiXL containing 300-350 amino acids). Purification of ei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::995128bc42abfde68c45aef305fa4c02
https://doi.org/10.1074/jbc.m306112200
https://doi.org/10.1074/jbc.m306112200
Publikováno v:
Journal of Biological Chemistry. 278:22388-22395
The cobaltochelatase required for the synthesis of vitamin B12 (cobalamin) in the archaeal kingdom has been identified as CbiX through similarity searching with the CbiX from Bacillus megaterium. However, the CbiX proteins in the archaea are much sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::185c3c3f43303b3ec4e22c6a3ba0ffa0
https://doi.org/10.1074/jbc.m302468200
https://doi.org/10.1074/jbc.m302468200
Publikováno v:
Biochemical Society Transactions. 30:610-613
One of the four operons required for cobalamin biosynthesis in Bacillus megaterium is also associated with sirohaem synthesis, and contains three genes, sirA, sirB and sirC. By undertaking functional complementation experiments and in vitro assays us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::161915adedf561eba3257c4fcd361ed3
https://doi.org/10.1042/bst0300610
https://doi.org/10.1042/bst0300610