Assessment of extracellular field potential and Ca2+ transient signals for early QT/pro-arrhythmia detection using human induced pluripotent stem cell-derived cardiomyocytes

Autor: Karen Oldman, Mark Pilling, Martin R. Brown, Chris Pollard, Najah Abi-Gerges, Clare E. Sefton, Amy Pointon, Helen Garside

Publikováno v: Journal of Pharmacological and Toxicological Methods. 83:1-15

Cardiovascular toxicity is a prominent reason for failures in drug development, resulting in the demand for assays that can predict this liability in early drug discovery. We investigated whether iCell® cardiomyocytes have utility as an early QT/TdP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eea9c3e623f2fd46b6667491ce171e8d
https://doi.org/10.1016/j.vascn.2016.09.001

Assessment of extracellular field potential and Ca

Autor: Najah, Abi-Gerges, Amy, Pointon, Karen L, Oldman, Martin R, Brown, Mark A, Pilling, Clare E, Sefton, Helen, Garside, Christopher E, Pollard

Publikováno v: Journal of pharmacological and toxicological methods. 83

Cardiovascular toxicity is a prominent reason for failures in drug development, resulting in the demand for assays that can predict this liability in early drug discovery. We investigated whether iCell® cardiomyocytes have utility as an early QT/TdP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4676831fce625efd0420851e06beea68
https://pubmed.ncbi.nlm.nih.gov/27622857

Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor

Autor: Fiona H Blackhall, Ian J. Stratford, Leo A. H. Zeef, Helen Garside, Anne White, Dave Singh, Lisa Rice, Charlotte E Waters, Adam Stevens, Paul Kay, David W. Ray, Robert Clarke, Jennifer Eccles, Brian A. Telfer, Paula Sommer

Publikováno v: The Journal of Endocrinology

Glucocorticoid (GC) receptors (GRs) have profound anti-survival effects on human small cell lung cancer (SCLC). To explore the basis of these effects, protein partners for GRs were sought using a yeast two-hybrid screen. We discovered a novel gene, F

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bcdd038734e3dcc787b5f6f92c0928f
https://doi.org/10.1677/joe-08-0019

An in Vitro Assay Using Overexpressed Yeast SRP Demonstrates that Cotranslational Translocation Is Dependent upon the J-Domain of Sec63p

Autor: Andrew Jermy, Stephanie Carter, Martin Willer, Helen Garside, Gregor J. Steel, Colin J. Stirling

Publikováno v: Biochemistry. 42:7171-7177

The signal recognition particle (SRP) is required for co-translational targeting of polypeptides to the endoplasmic reticulum (ER). Once at the membrane, the precursor interacts with a complex proteinaceous machinery that mediates its translocation a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fe7be3748a41f8048e4995e304d8b28
https://doi.org/10.1021/bi034395l

Caveolin mediates rapid glucocorticoid effects and couples glucocorticoid action to the antiproliferative program

Autor: Andrew Berry, David W. Ray, Laura Matthews, Jacqueline Ohanian, Helen Garside, Vasken Ohanian

Publikováno v: Matthews, L, Berry, A, Ohanian, V, Ohanian, J, Garside, H & Ray, D 2008, ' Caveolin mediates rapid glucocorticoid effects and couples glucocorticoid action to the antiproliferative program ', Molecular Endocrinology, vol. 22, no. 6, pp. 1320-1330 . https://doi.org/10.1210/me.2007-0154

Many glucocorticoid (Gc) actions are of rapid onset and therefore require acute regulation of intracellular signaling cascades. Integration of diverse extracellular signals requires cross-talk between intracellular pathways, suggesting the existence

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a598fd4fccf76f365e626cccaf33b7c
https://pubmed.ncbi.nlm.nih.gov/18308897