Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Helen A. Vincent"'
Autor:
Charlotte E. Mardle, Thomas J. Shakespeare, Louise E. Butt, Layla R. Goddard, Darren M. Gowers, Helen S. Atkins, Helen A. Vincent, Anastasia J. Callaghan
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Abstract Regulation of gene expression through processing and turnover of RNA is a key mechanism that allows bacteria to rapidly adapt to changing environmental conditions. Consequently, RNA degrading enzymes (ribonucleases; RNases) such as the endor
Externí odkaz:
https://doaj.org/article/29bc3d3fc5174804a0b59cfe01838ac0
Autor:
Charlotte E. Mardle, Layla R. Goddard, Bailei C. Spelman, Helen S. Atkins, Louise E. Butt, Paul A. Cox, Darren M. Gowers, Helen A. Vincent, Anastasia J. Callaghan
Publikováno v:
Biochemistry and Biophysics Reports, Vol 23, Iss , Pp 100773- (2020)
Increasing resistance of bacteria to antibiotics is a serious global challenge and there is a need to unlock the potential of novel antibacterial targets. One such target is the essential prokaryotic endoribonuclease RNase E. Using a combination of i
Externí odkaz:
https://doaj.org/article/39b31f13db9045beba6bd2b54776db65
Autor:
Layla R. Goddard, Charlotte E. Mardle, Hassan Gneid, Ciara G. Ball, Darren M. Gowers, Helen S. Atkins, Louise E. Butt, Jonathan K. Watts, Helen A. Vincent, Anastasia J. Callaghan
Publikováno v:
Molecules, Vol 26, Iss 11, p 3414 (2021)
The increase in antibacterial resistance is a serious challenge for both the health and defence sectors and there is a need for both novel antibacterial targets and antibacterial strategies. RNA degradation and ribonucleases, such as the essential en
Externí odkaz:
https://doaj.org/article/b863b08a28cc4dc7a0607f5fb37917eb
Autor:
Arqam Alomari, Robert Gowland, Callum Southwood, Jak Barrow, Zoe Bentley, Jashel Calvin-Nelson, Alice Kaminski, Matthew LeFevre, Anastasia J. Callaghan, Helen A. Vincent, Darren M. Gowers
Publikováno v:
Molecules, Vol 26, Iss 9, p 2508 (2021)
Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair. Eubacterial DNA ligases u
Externí odkaz:
https://doaj.org/article/9fc2d848c44846f09d6952ec3d521ea7
Autor:
Timothy J. Ragan, Helen A. Vincent
Publikováno v:
Methods in Molecular Biology ISBN: 9781071630037
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6c0cb624c493fd0cd222f018a21a460
https://doi.org/10.1007/978-1-0716-3004-4_6
https://doi.org/10.1007/978-1-0716-3004-4_6
Publikováno v:
Methods in Molecular Biology ISBN: 9781071630037
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c957e26ac40c14aea151af39d18e38b9
https://doi.org/10.1007/978-1-0716-3004-4_13
https://doi.org/10.1007/978-1-0716-3004-4_13
Publikováno v:
Henderson, C A, Vincent, H A & Callaghan, A J 2021, ' Reprogramming gene expression by targeting RNA-based interactions: a novel pipeline utilizing RNA array technology ', ACS Synthetic Biology . https://doi.org/10.1021/acssynbio.0c00603
Regulatory RNA-based interactions are critical for coordinating gene expression and are increasingly being targeted in synthetic biology, antimicrobial, and therapeutic fields. Bacterial trans-encoded small RNAs (sRNAs) regulate the translation and/o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10c81d95ae05612f3be717506ada56b7
https://researchportal.port.ac.uk/ws/files/28754461/Reprograming_Gene_Expression.pdf
https://researchportal.port.ac.uk/ws/files/28754461/Reprograming_Gene_Expression.pdf
Autor:
Jashel Calvin-Nelson, Robert Michael Nicholas Gowland, Zoe Bentley, Jak Barrow, Helen A. Vincent, Darren M. Gowers, Anastasia J. Callaghan, Matthew LeFevre, Alice Kaminski, Arqam Mohamad Azhar Msbah Alomari, Callum Southwood
Publikováno v:
Molecules, Vol 26, Iss 2508, p 2508 (2021)
Molecules
Volume 26
Issue 9
Alomari, A M A M, Gowland, R M N, Southwood, C, Barrow, J, Bentley, Z, Calvin-Nelson, J, Kaminski, A, LeFevre, M, Callaghan, A, Vincent, H & Gowers, D 2021, ' Identification of novel inhibitors of Escherichia coli DNA ligase ', Molecules, vol. 26, no. 9, 2508 . https://doi.org/10.3390/molecules26092508
Molecules
Volume 26
Issue 9
Alomari, A M A M, Gowland, R M N, Southwood, C, Barrow, J, Bentley, Z, Calvin-Nelson, J, Kaminski, A, LeFevre, M, Callaghan, A, Vincent, H & Gowers, D 2021, ' Identification of novel inhibitors of Escherichia coli DNA ligase ', Molecules, vol. 26, no. 9, 2508 . https://doi.org/10.3390/molecules26092508
Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair. Eubacterial DNA ligases u
Autor:
Helen A. Vincent, Callum A. Rail, Anastasia J. Callaghan, Louise E. Butt, Charlotte A. Henderson
Publikováno v:
Methods (San Diego, Calif.)
Henderson, C, Rail, C, Butt, L, Vincent, H & Callaghan, A 2019, ' Generation of small molecule-binding RNA arrays and their application to fluorogen-binding RNA aptamers ', Methods, vol. 167, pp. 39-53 . https://doi.org/10.1016/j.ymeth.2019.04.021
Henderson, C, Rail, C, Butt, L, Vincent, H & Callaghan, A 2019, ' Generation of small molecule-binding RNA arrays and their application to fluorogen-binding RNA aptamers ', Methods, vol. 167, pp. 39-53 . https://doi.org/10.1016/j.ymeth.2019.04.021
Highlights • Techniques for quantitative in vitro analysis of fluorogen-RNA aptamers are needed. • We have recently developed a novel method for the production of functional-RNA arrays. • A protocol for the production of fluorogen-binding RNA a
Publikováno v:
Norouzi, M, Pickford, A, Butt, L, Vincent, H & Callaghan, A 2019, ' Application of mRNA arrays for the production of mCherry reporter-protein arrays for quantitative gene expression analysis ', ACS Synthetic Biology . https://doi.org/10.1021/acssynbio.8b00266
The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene