Zobrazeno 1 - 10
of 65
pro vyhledávání: '"Heitkamp, Thomas"'
Autor:
Spantzel, Lukas, Pérez, Iván, Heitkamp, Thomas, Westphal, Anika, Reuter, Stefanie, Mrowka, Ralf, Börsch, Michael
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor. The receptor is activated by a small peptide ligand neurotensin. NTSR1 can be expressed in HEK cells by stable transfection. Previously we used the fluorescent protein markers
Externí odkaz:
http://arxiv.org/abs/2302.02416
Autor:
Heitkamp, Thomas, Börsch, Michael
FoF1-ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rotation, by substrate and product concentrations, by mechanical inhibitor
Externí odkaz:
http://arxiv.org/abs/2103.11986
Autor:
Dathe, André, Heitkamp, Thomas, Pérez, Iván, Sielaff, Hendrik, Westphal, Anika, Reuter, Stefanie, Mrowka, Ralf, Börsch, Michael
G protein-coupled receptors (GPCRs) are a large superfamily of membrane proteins that are activated by extracellular small molecules or photons. Neurotensin receptor 1 (NTSR1) is a GPCR that is activated by neurotensin, i.e. a 13 amino acid peptide.
Externí odkaz:
http://arxiv.org/abs/1902.01511
Autor:
Westphal, Anika, Sielaff, Hendrik, Reuter, Stefanie, Heitkamp, Thomas, Mrowka, Ralf, Börsch, Michael
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that can be expressed in HEK293T cells by stable transfection. Its ligand is a 13-amino-acid peptide that binds with nanomolar affinity from the extracellular side to NTSR1. Lig
Externí odkaz:
http://arxiv.org/abs/1901.11246
Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET
Neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that is important for signaling in the brain and the gut. Its agonist ligand neurotensin (NTS), a 13-amino-acid peptide, binds with nanomolar affinity from the extracellular side to NTSR1
Externí odkaz:
http://arxiv.org/abs/1801.09318
Autor:
Foertsch, Franziska, Ilchenko, Mykhailo, Heitkamp, Thomas, Nossmann, Silke, Hoffmann, Birgit, Starke, Ilka, Mrowka, Ralf, Biskup, Christoph, Borsch, Michael
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhi
Externí odkaz:
http://arxiv.org/abs/1702.00512
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a
Externí odkaz:
http://arxiv.org/abs/1701.03422
Adenosine triphosphate (ATP) is the universal chemical energy currency for cellular activities provided mainly by the membrane enzyme FoF1-ATP synthase in bacteria, chloroplasts and mitochondria. Synthesis of ATP is accompanied by subunit rotation wi
Externí odkaz:
http://arxiv.org/abs/1602.04235
Autor:
Dienerowitz, Maria, Ilchenko, Mykhailo, Su, Bertram, Deckers-Hebestreit, Gabriele, Mayer, Guenter, Henkel, Thomas, Heitkamp, Thomas, Börsch, Michael
Observation times of freely diffusing single molecules in solution are limited by the photophysics of the attached fluorescence markers and by a small observation volume in the femtolitre range that is required for a sufficient signal-to-background r
Externí odkaz:
http://arxiv.org/abs/1601.06930
Autor:
Su, Bertram, Dueser, Monika G., Zarrabi, Nawid, Heitkamp, Thomas, Starke, Ilka, Börsch, Michael
To monitor conformational changes of individual membrane transporters in liposomes in real time, we attach two fluorophores to selected domains of a protein. Sequential distance changes between the dyes are recorded and analyzed by Forster resonance
Externí odkaz:
http://arxiv.org/abs/1502.04065