Phosphopeptide Enrichment by Covalent Chromatography after Derivatization of Protein Digests Immobilized on Reversed-Phase Supports

Autor: Edward Nieves, Heinz Nika, Ruth Hogue Angeletti, David H. Hawke

Publikováno v: Journal of Biomolecular Techniques : JBT. 24:154-177

A rugged sample-preparation method for comprehensive affinity enrichment of phosphopeptides from protein digests has been developed. The method uses a series of chemical reactions to incorporate efficiently and specifically a thiol-functionalized aff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121ccafae9806ae2d2499289a423a3e0
https://doi.org/10.7171/jbt.13-2403-004

EGF-Induced ERK Activation Promotes CK2-Mediated Disassociation of α-Catenin from β-Catenin and Transactivation of β-Catenin

Autor: David H. Hawke, Ji Wang, Haitao Ji, Xuexun Fang, David W. Litchfield, Kenneth Aldape, Dexing Fang, Qingyuan Ge, Susan Keezer, Zhimin Lu, Heinz Nika, Bingliang Fang

Publikováno v: Biochemistry Publications

Increased transcriptional activity of beta-catenin resulting from Wnt/Wingless-dependent or -independent signaling has been detected in many types of human cancer, but the underlying mechanism of Wnt-independent regulation remains unclear. We demonst

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cc60558e940b9561314e9753490bd8f
https://doi.org/10.1016/j.molcel.2009.09.034

N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization

Autor: Ruth Hogue Angeletti, Heinz Nika, David H. Hawke

Publikováno v: Journal of biomolecular techniques : JBT. 25(1)

A sample preparation method for protein N-terminal peptide isolation from cyanogen bromide (CNBr) protein digests has been developed. In this strategy, the CNBr cleavage was preceded by protein α- and e-amine acetylation and carboxyamidomethylation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f92a4bcdbb689e84eaf0cbf68dc0f986
https://pubmed.ncbi.nlm.nih.gov/24688320

Optimization of the β-Elimination/Michael Addition Chemistry on Reversed-Phase Supports for Mass Spectrometry Analysis of O-Linked Protein Modifications

Autor: Ruth Hogue Angeletti, David H. Hawke, Edward Nieves, Heinz Nika

We previously adapted the β-elimination/Michael addition chemistry to solid-phase derivatization on reversed-phase supports, and demonstrated the utility of this reaction format to prepare phosphoseryl peptides in unfractionated protein digests for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::135995aa5123d79eb521cce89bb7da4c
https://europepmc.org/articles/PMC3703673/

MPSA short communications

Autor: Eugene M. Barnes, Patricia A. Calkin, Satoshi Kuroda, Shigemi Norioka, Masanori Mitta, Ikunoshin Kato, Fumio Sakiyama, Heinz Nika, David T. Chow, Daniel Hess, Edward J. Bures, Hamish D. Morrison, Ruedi Aebersold, G. Marius Clore, Angela M. Gronenborn, Bengt Persson, Patrick Argos, Peter James, Andrew C. Cannons, Larry P. Solomonson, Kenneth E. Dombrowski, William E. Moddeman, Stephen E. Wright, Winona C. Barker, David G. George, Subhendra N. Mattagajasingh, Hara P. Misra, Shuan Shian Huang, Jung San Huang, Y. C. Lee, Wolfgang H. Fischer, A. Grey Craig, Philip N. McFadden, Jonathan A. Lind-quist, M. Bartlet-Jones, W. Jeffery, H. F. Hansen, D. J. C. Pappin, Tomas Bergman, Lars Hjelmqvist, Mats Estonius, Hans Jörnvall, Donna S. Dorow, H. Tschesche, V. Knäuper, T. Kleine, P. Reinemer, S. Schnierer, F. Grams, W. Bode, Christopher Southan, Kenneth Fantom, Patric Lavery, J. B. C. Findlay, D. Akrigg, T. K. Attwood, M. J. Beck, A. J. Bleasby, A. C. T. North, D. J. Parry-Smith, D. N. Perkins, A. Aitken, Y. Patel, H. Martin, D. Jones, K. Robinson, J. Madrazo, S. Howell, Tom Yungwirth, Michael Affolter, Lawrence Amankwa, Harold A. Scheraga, Chao -Yuh Yang, Natalia V. Valentinova, Manlan Yang, Zi -Wei Gu, Antonio M. Gotto, Norman J. Dovichi, Karen C. Waldron, Min Chen, Ian Ireland, Akira Omori, Sachiyo Yoshida, Johann Schaller, Stephan Lengweiler, Egon E. Rickli, José Bubis, Julio O. Ortiz, Carolina Möller, Enrique J. Millán, Victoria L. Boyd, MeriLisa Bozzini, Jindong Zhao, Robert J. DeFranco, Pau -Miau Yuan, G. Marc Loudon, Duy Nguyen, Masaharu Kamo, Takao Kawakami, Norifumi Miyatake, Akira Tsugita, Keiji Takamoto, Kazuo Satake, Oliver Bischof, Mirko Hechenberger, Bernd Thiede, Volker Kruft, Brigitte Wittmann-Liebold, Albrecht Otto, Rainer Benndorf, Peter Jungblut, Monika Ühlein, Henning Urlaub, Rita Berhardt, Regine Kraft, Heike Uhlmann, Vita Beckert, Toshifumi Akizawa, Takaaki Ayabe, Motomi Matsukawa, Michiyasu Itoh, Masatoshi Nishi, Hiroshi Sato, Motoharu Seiki, Masanori Yoshioka, Michal Lebl, Viktor Krchňák, Nikolai F. Sepetov, Petr Kočiš, Marcel Pátek, Zuzana Flegelová, Ronald Ferguson, Kit S. Lam, Robert L. Moritz, James Eddes, Hong Ji, Gavin E. Reid, Richard J. Simpson, William Seffens, C. Dale Poulter, Julia M. Dolence, Pamela D. Bond, Kiyoshi Nokihara, Kazuo Ikegaya, Naoki Morita, Takao Ohmura, S. I. Salikhov, N. J. Sagdiev, A. S. Korneev, Behzod Z. Dolimbek, M. Zhouhair Atassi, J. S. Rosenberg, Z. Yun, P. R. Wyde, M. Z. Atassi, Simon J. Gaskell, Kalyan Rao Anumula, David P. Goldenberg, Ettore Appella, Michelle Fiscella, Nicola Zambrano, Stephen J. Ullrich, Kazuyasu Sakaguchi, Hiroshi Sakamoto, Marc S. Lewis, David Lin, W. Edward Mercer, Carl W. Anderson, Marjorie A. Connelly, Hong Zhang, John D. Sipley, Susan P. Lees-Miller, Stephen P. Jackson, Yong-hong Xie, Jun A. Quion, Chao-yuh Yang, W. F. Brandt, H. Alk, R. Bhaskaran, Chin Yu, C. C. Yang, Agnes H. Henschen, Keith Ashman, Matthias Mann, Juan Guevara, Hung Michael Nguyen, Daniel B. Davison, Joel D. Morrisett, Richard N. Perham, Donald A. Marvin, Martyn F. Symmons, Tamsin D. Terry, Z. H. Beg, J. A. Stonik, J. M. Hoeg, H. B. Brewer, Boris M. Gorovits, C. S. Raman, Paul M. Horowtiz

Publikováno v: Journal of Protein Chemistry. 13:431-512

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5ef34d6537f2c82a8b9b3644d6d48729
https://doi.org/10.1007/bf01898855