Ribosomal crystallography: a flexible nucleotide anchoring tRNA translocation, facilitates peptide-bond formation, chirality discrimination and antibiotics synergism

Autor: Ilana Agmon, Joerg Harms, Maggie Kessler, David Baram, Assa Sittner, Ada Yonath, Maya Amit, Inbal Greenberg, Heike Bartels, Harly A. S. Hansen, Erez Pyetan, Tamar Auerbach, Rita Berisio, Frank Schluenzen, Anat Bashan, Raz Zarivach

Publikováno v: FEBS letters
567 (2004): 20–25. doi:10.1016/j.febslet.2004.03.065
info:cnr-pdr/source/autori:Agmon, Ilana; Amit, Maya; Auerbach, Tamar; Bashan, Anat; Baram, David; Bartels, Heike; Berisio, Rita; Greenberg, Inbal; Harms, Joerg; Hansen, Harly A. S.; Kessler, Maggie; Pyetan, Erez; Schluenzen, Frank; Sittner, Assa; Yonath, Ada; Zarivach, Raz./titolo:Ribosomal crystallography: a flexible nucleotide anchoring tRNA translocation, facilitates peptide-bond formation, chirality discrimination and antibiotics synergism./doi:10.1016%2Fj.febslet.2004.03.065/rivista:FEBS letters (Print)/anno:2004/pagina_da:20/pagina_a:25/intervallo_pagine:20–25/volume:567

The linkage between internal ribosomal symmetry and transfer RNA (tRNA) positioning confirmed positional catalysis of amino-acid polymerization. Peptide bonds are formed concurrently with tRNA-3′end rotatory motion, in conjunction with the overall

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::881b331be8411dc11dce9855ad1ac70f
https://doi.org/10.1016/j.febslet.2004.03.065

Identification of Selected Ribosomal Components in Crystallographic Maps of Prokaryotic Ribosomal Subunits at Medium Resolution

Autor: Inna Levin, Maggie Kessler, Moshe Peretz, Daniela Janell, Marta Pioletti, Frank Schluenzen, W. S. Bennett, Anat Bashan, Horacio Avila, M. Gluehmann, Heike Bartels, Ante Tocilji, Ilana Agmon, Joerg Harms, Francois Franceschi, Shulamith Weinstein, Ada Yonath, Maria Simitsopoulou, Tamar Auerbach, Harly A. S. Hansen

Publikováno v: The Ribosome

This chapter focuses on the electron density map of the small ribosomal subunit, shows features interpreted as ribosomal proteins and rRNA, and pinpoints secondary-structure elements. It highlights the use of heavy-atom markers for unbiased targeting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::41b79d4dfa6e571d744fa6455d5284f9
https://doi.org/10.1128/9781555818142.ch3

Ribosomal Crystallography: From Poorly Diffracting Microcrystals to High-Resolution Structures

Autor: M. Gluehmann, Anat Bashan, Harly A. S. Hansen, Tamar Auerbach, Joerg Harms, Ada Yonath, Gabriel Rosenblum, Frank Schluenzen, Heike Bartels, Ilana Agmon, Marta Pioletti, Horacio Avila, Raz Zarivach, Francois Franceschi

Publikováno v: Methods. 25:292-302

The cellular organelles translating the genetic code into proteins, the ribosomes, are large, asymmetric, flexible, and unstable ribonucleoprotein assemblies, hence they are difficult to crystallize. Despite two decades of intensive effort and thorou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52867401817a503df9066d34feb8547e
https://doi.org/10.1006/meth.2001.1241

The identification of selected components in electron density maps of prokaryotic ribosomes at 7 Å resolution

Autor: Maggie Kessler, Daniela Janell, Anat Bashan, Frank Schlünzen, Marco Glühmann, Ante Tocilj, J. Harms, Harly A. S. Hansen, Heike Bartels, Francois Franceschi, Tamar Auerbach, Moshe Peretz, Ilana Agmon, W. S. Bennett, Shulamith Weinstein, Inna Levin, Horacio Avila, Ada Yonath, Marta Pioletti, Susanne Krumbholz, Kostas Anagnostopoulos, Ingo Kölln

Publikováno v: Journal of Synchrotron Radiation. 6:928-941

Crystals of small and large ribosomal subunits from thermophilic and halophilic bacteria, diffracting to 3 A, are being subjected to structural analysis with synchrotron radiation. The bright beam necessary for detecting and collecting the diffractio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::020b98e9f009dce18182539dbfd3a5f6
https://doi.org/10.1107/s0909049599006664

A milestone in ribosomal crystallography: the construction of preliminary electron density maps at intermediate resolution

Autor: Francois Franceschi, M. Geva, Z. Berkovitch-Yellin, J. Harms, Irit Sagi, S. Krumbholz, Ilana Agmon, Harly A. S. Hansen, S. Morlang, Nina Böddeker, A. Zaytzev-Bashan, Moshe Peretz, A. Dribin, V. Weinrich, Shulamith Weinstein, R. Sharon, N. Volkmann, Ada Yonath, Frank Schlünzen, E. Maltz, Heike Bartels, J. Thygesen, W. S. Bennett, Itay Levin

Publikováno v: Biochemistry and Cell Biology. 73:739-749

Preliminary electron density maps of the large and the small ribosomal particles from halophilic and thermophilic sources, phased by the isomorphous replacement method, have been constructed at intermediate resolution. These maps contain features com

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84226e8d0bf1b609063d2b703349446d
https://doi.org/10.1139/o95-082