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pro vyhledávání: '"Heidrun Heilmann"'
Publikováno v:
Molecular and General Genetics MGG. 178:149-154
P1 infected minicells synthesize approximately 50 phage-encoded polypeptides. Phage expression is temporally controlled, demonstrating phage polypeptides synthesized both early and late after infection. The P1 repressor, gpcl1 (Mr = 33,000), represso
Publikováno v:
Journal of Molecular Biology. 144:387-396
The gene encoding the restriction activity of the restriction-modification enzyme EcoPI has been delineated by transposon mutagenesis. Insertion of Tn1 or Tn5 into this non-essential gene has been used to identify the gene product, gpres, as a polype
Autor:
Heidrun Heilmann, John N. Reeve
Publikováno v:
Gene. 17(1)
A unique BamHI restriction site has been inserted into a nonessential region of the genome of a deletion mutant of phage SPP1. Construction of this phage, designated SPP1 v, required the in vitro conversion of a BclI site to a BamHI site. SPP1 v has