Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Hector Juarez-Salinas"'
Autor:
Hector Juarez-Salinas, Susan M. Scott
Publisher Summary This chapter discusses the purification and quantitation of monoclonal antibodies by affinity chromatography with immobilized protein A. The availability of the MAPS process has also made it possible to use protein A chromatography
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2d648ede6f6a4567cab865a8917b78a2
https://doi.org/10.1016/b978-0-12-123012-8.50023-5
https://doi.org/10.1016/b978-0-12-123012-8.50023-5
Publikováno v:
Analytical Biochemistry. 106:296-306
A new method for the measurement of poly(ADP-ribose) is described. Poly(ADP-ribose) was separated from the bulk of cellular RNA and DNA by quantitative adsorption to dihydroxyboryl-Sepharose. The polymer was digested with venom phosphodiesterase and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eb32f9266ce62bdbc3576d388b8309b
https://doi.org/10.1016/0003-2697(80)90524-2
https://doi.org/10.1016/0003-2697(80)90524-2
Publikováno v:
Journal of Immunological Methods. 76:157-169
A single-step method for purification of mouse monoclonal antibodies directly from ascitic fluids using hydroxylapatite column chromatography is described. The procedure yields highly purified IgG or IgM antibodies. The purified immunoglobulin is ess
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dce28f6bd0f42e4ef78a570097dbec4e
https://doi.org/10.1016/0022-1759(85)90488-0
https://doi.org/10.1016/0022-1759(85)90488-0
Publikováno v:
Analytical Biochemistry. 135:69-77
Three different immobilized boronates were compared with respect to their possible utility in studies of adenine and pyridine nucleotide metabolism. These included boronate derivatives of polyacrylamide, Sepharose, and the cation-exchange resin, Bio-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::390f7bc98317fb4a90f8a064e1f6229b
https://doi.org/10.1016/0003-2697(83)90732-7
https://doi.org/10.1016/0003-2697(83)90732-7
Publikováno v:
Nature. 282(5740)
Several reports indicate that intracellular levels of NAD are rapidly decreased by DNA-damaging agents such as strep-tozotocin1–7, neocarzinostatin8,9 and ionising radiation8–11. Similarly, evidence obtained from studies in isolated nuclei and pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce4db7e7ae28c05739ff8fbdcdccc0ec
https://pubmed.ncbi.nlm.nih.gov/229416
https://pubmed.ncbi.nlm.nih.gov/229416
Autor:
Rafael Alvarez-Gonzalez, Hector Juarez-Salinas, James L. Sims, Myron K. Jacobson, D. Michael Payne, Elaine L. Jacobson
Publisher Summary This chapter describes the determination of in vivo levels of polymeric and monomeric ADP-ribose by fluorescence methods. Two key features that are common to both methods and are crucial in providing the necessary selectivity and se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6caffeb648c420b1eaccbb751944a04e
https://doi.org/10.1016/0076-6879(84)06052-3
https://doi.org/10.1016/0076-6879(84)06052-3
Autor:
Viktorya Levi, Elaine L. Jacobson, Myron K. Jacobson, Hector Juarez-Salinas, Hilda Mendoza-Alvarez
Publikováno v:
Analytical biochemistry. 131(2)
Methodology for the routine and simultaneous determination of the linear and branched residues of poly(ADP-ribose) is described. The main features of the procedure consist of the isolation of poly(ADP-ribose) by affinity chromatography; enzymatic dig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::344102851887a0066dfbe4ee6a1ab2ca
https://pubmed.ncbi.nlm.nih.gov/6311052
https://pubmed.ncbi.nlm.nih.gov/6311052
Publikováno v:
Proceedings in Life Sciences ISBN: 9783642705915
The nuclear metabolism of poly(ADP-ribose) involves at least three enzymatic activities, poly(ADP-ribose) polymerase, poly(ADP-ribose) glycohydrolase, and protein-mono(ADP-ribose) lyase. Although each of these enzymes has been purified and studied, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::21cbb6eab76790aa10699b8d46744e28
https://doi.org/10.1007/978-3-642-70589-2_39
https://doi.org/10.1007/978-3-642-70589-2_39
Publikováno v:
Biochemical and biophysical research communications. 122(3)
The intracellular levels of poly(ADP-ribose) in cultured mouse cells were increased in response to hyperthermic treatment (43°C). When hyperthermia was combined with other stressful treatments such as with ethanol and/or an alkylating agent, a drama
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6c18873b865c97f8098226bf7aab1b
https://pubmed.ncbi.nlm.nih.gov/6089782
https://pubmed.ncbi.nlm.nih.gov/6089782