Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Heather Rada"'
Autor:
Jennifer Timm, Cristina Bosch-Navarrete, Eliseo Recio, Joanne E Nettleship, Heather Rada, Dolores González-Pacanowska, Keith S Wilson
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 9, Iss 5, p e0003781 (2015)
Leishmania spp. is a protozoan parasite and the causative agent of leishmaniasis. Thymidine kinase (TK) catalyses the transfer of the γ-phosphate of ATP to 2'-deoxythymidine (dThd) forming thymidine monophosphate (dTMP). L. major Type II TK (LmTK) h
Externí odkaz:
https://doaj.org/article/3756b357c9194de8b705af699ce4ab89
Publikováno v:
The Febs Journal
Cyclic guanosine 3′,5′‐monophosphate (cGMP) is an intracellular signalling molecule involved in many sensory and developmental processes. Synthesis of cGMP from GTP is catalysed by guanylate cyclase (GC) in a reaction analogous to cAMP formatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03361da954cea477b0e2ea2ae9b6251e
http://ora.ox.ac.uk/objects/uuid:
http://ora.ox.ac.uk/objects/uuid:
Autor:
Joanne E. Nettleship, Heather Rada, Humberto D'Muniz Pereira, Yamini Reddivari, Louise E. Bird, Adriano de Freitas Fernandes, José Brandão-Neto, Raymond J. Owens, Ana Eliza Zeraik, Juliana Roberta Torini, L. Romanello, Ricardo DeMarco, Vitor Serrão
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
Schistosoma mansoni, the parasite responsible for schistosomiasis, lacks the “de novo” purine biosynthetic pathway and depends entirely on the purine salvage pathway for the supply of purines. Numerous reports of praziquantel resistance have been
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1b940efe6d97fbb60027b9b8ae61727
https://pubmed.ncbi.nlm.nih.gov/30772423
https://pubmed.ncbi.nlm.nih.gov/30772423
Autor:
Keith S. Wilson, Joanne E. Nettleship, Tom Balzarini, Heather Rada, Víctor M. Castillo-Acosta, Maria João Valente, Dolores González-Pacanowska, Louise E. Bird, Luis M. Ruiz-Pérez, Jennifer Timm
Publikováno v:
Molecular Microbiology. 102:365-385
Thymidine kinase (TK) is a key enzyme in the pyrimidine salvage pathway which catalyzes the transfer of the γ-phosphate of ATP to 2′-deoxythymidine (dThd) forming thymidine monophosphate (dTMP). Unlike other type II TKs, the Trypanosoma brucei enz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c41baf6fa35d1e2bf030885b8f170f7
https://doi.org/10.1111/mmi.13467
https://doi.org/10.1111/mmi.13467
Autor:
Joanne E. Nettleship, Raymond J. Owens, Louise E. Bird, Heather Rada, José Brandão-Neto, Humberto D'Muniz Pereira, Ricardo DeMarco, Juliana Roberta Torini, Vitor Serrão, L. Romanello, Yamini Reddivari
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
Schistosoma mansoni is the parasite responsible for schistosomiasis, a disease that affects about 218 million people worldwide. Currently, both direct treatment and disease control initiatives rely on chemotherapy using a single drug, praziquantel. C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75ea5ecde9378214b00218d009c471c1
https://pubmed.ncbi.nlm.nih.gov/28347672
https://pubmed.ncbi.nlm.nih.gov/28347672
Autor:
Louise E, Bird, Joanne E, Nettleship, Valtteri, Järvinen, Heather, Rada, Anil, Verma, Raymond J, Owens
Publikováno v:
Advances in experimental medicine and biology. 922
The production of recombinant integral membrane proteins for structural and functional studies remains technically challenging due to their relatively low levels of expression. To address this problem, screening strategies have been developed to iden
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5aa857c79ddacc3126d5d4d57e129be7
https://pubmed.ncbi.nlm.nih.gov/27553231
https://pubmed.ncbi.nlm.nih.gov/27553231
Autor:
Louise E. Bird, J.M. Diprose, Raymond J. Owens, Heather Rada, John G. Flanagan, Robert J.C. Gilbert
Publikováno v:
DNA Cloning and Assembly Methods ISBN: 9781627037631
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0582a7cb8ef67f9cabe1de68757f015
https://doi.org/10.1007/978-1-62703-764-8_15
https://doi.org/10.1007/978-1-62703-764-8_15
Autor:
Maria, Valente, Jennifer, Timm, Víctor M, Castillo-Acosta, Luis M, Ruiz-Pérez, Tom, Balzarini, Joanne E, Nettleship, Louise E, Bird, Heather, Rada, Keith S, Wilson, Dolores, González-Pacanowska
Publikováno v:
Molecular microbiology. 102(3)
Thymidine kinase (TK) is a key enzyme in the pyrimidine salvage pathway which catalyzes the transfer of the γ-phosphate of ATP to 2'-deoxythymidine (dThd) forming thymidine monophosphate (dTMP). Unlike other type II TKs, the Trypanosoma brucei enzym
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::69f7461cb98aaac4191f004675615946
https://pubmed.ncbi.nlm.nih.gov/27426054
https://pubmed.ncbi.nlm.nih.gov/27426054
Autor:
Jan Lӧwe, Heather Rada, Anil Verma, Louise E. Bird, Matthew Jennions, Isabel Moraes, Raymond J. Owens, Raphael Gasper, James M. Birch
Publikováno v:
Journal of Visualized Experiments.
The production of recombinant membrane proteins for structural and functional studies remains technically challenging due to low levels of expression and the inherent instability of many membrane proteins once solubilized in detergents. A protocol is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45a53c3c0fa3e17212843fdf35859736
https://doi.org/10.3791/52357
https://doi.org/10.3791/52357
Autor:
Joanne E. Nettleship, So Iwata, Heather Rada, Nien-Jen Hu, Alexander D. Cameron, Raymond J. Owens, Nahid Rahman, Louise E. Bird, David A. Drew
Publikováno v:
Methods in Molecular Biology ISBN: 9781493922291
A key step in the production of recombinant membrane proteins for structural studies is the optimization of protein yield and quality. One commonly used approach is to fuse the protein to green fluorescent protein (GFP), enabling expression to be tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd5520c93018096ceda4687da6c3d7fd
http://ora.ox.ac.uk/objects/uuid:24db906b-f7a7-4c7a-9796-1a5acbcbdd7e
http://ora.ox.ac.uk/objects/uuid:24db906b-f7a7-4c7a-9796-1a5acbcbdd7e