Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Heather R. Harke"'
Autor:
Heather R. Harke, Jian Ying Zhao, Norman J. Dovichi, Jean Miller, Jian Zhong Zhang, Karen C. Waldron
Publikováno v:
Journal of Chromatography A. 608:239-242
Complicated electropherograms are produced in the separation of fluorescently labeled peptides. Incomplete labeling of epsilon-amino groups on lysine residues results in the production of 2n-1 reaction products, where n is the number of alpha and eps
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9d165ed15d84713de8f8f9c538d794a
https://doi.org/10.1016/0021-9673(92)87129-v
https://doi.org/10.1016/0021-9673(92)87129-v
Publikováno v:
Journal of Chromatography A. 608:143-150
Polyacrylamide capillary gels were prepared with constant (5% C) cross-linker concentration and with total acrylamide concentration ranging from 2.5 to 6% T. At each acrylamide concentration, peak spacing was constant for DNA sequencing fragments ran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2c81e8a88a102f6383aaca85f994749f
https://doi.org/10.1016/0021-9673(92)87116-p
https://doi.org/10.1016/0021-9673(92)87116-p
Autor:
Norman J. Dovichi, Da Yong Chen, Ronda. Grey, Harold Swerdlow, Shaole Wu, Heather R. Harke, Jian Zhong Zhang, Carl. Fuller
Publikováno v:
Analytical Chemistry. 63:2835-2841
Capillary gel electrophoresis is demonstrated for the four-spectral-channel sequencing technique of Smith, the two-spectral-channel sequencing technique of Prober, and the one-spectral-channel sequencing technique of Richardson and Tabor. Sequencing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::856f1a87ee65054857dbdf1ea76de07a
https://doi.org/10.1021/ac00024a006
https://doi.org/10.1021/ac00024a006
Publikováno v:
Analytical Sciences. 7:1485-1489
Detection limits of one to two thousand analyte molecules is produced by laser-induced fluorescence with the sheath flow cuvette for DNA sequencing. Two different detector systems are developed for the sequencing of real DNA samples: a single laser a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71e1cf47b9d6b77967ae409079ad585d
https://doi.org/10.2116/analsci.7.supple_1485
https://doi.org/10.2116/analsci.7.supple_1485
Publikováno v:
Journal of Chromatography A. 516:61-67
Capillary polyacrylamide gel electrophoresis separation of dideoxycytidine chain-terminated DNA fragments is reported. A post-column laser-induced fluorescence detector based on the sheath flow cuvette was used to minimize background signals due to l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6c9543e6574f5fb68b9ff6f333b4c16
https://doi.org/10.1016/s0021-9673(01)90204-3
https://doi.org/10.1016/s0021-9673(01)90204-3
Publikováno v:
Electrophoresis. 11:777-780
Capillary zone electrophoresis is employed for the separation and analysis of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids. Detection of minute amounts of these amino acid derivatives is an impor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86838948438e23bbbe837a49f9d0595b
https://doi.org/10.1002/elps.1150110918
https://doi.org/10.1002/elps.1150110918
Publikováno v:
Nucleic acids research. 20(18)
We report a modification to the peak-height encoded DNA sequencing technique of Tabor and Richardson. As in the original protocol, the sequencing reaction uses modified T7 polymerase with manganese rather than magnesium to produce very uniform incorp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6514548b6dc64af6618f47b3c323c868
https://pubmed.ncbi.nlm.nih.gov/1408803
https://pubmed.ncbi.nlm.nih.gov/1408803
Publikováno v:
Electrophoresis. 13(8)
Compressions are occasionally found during the separation of DNA sequencing fragments, particularly in G/C-rich regions and in gels operated at room temperature. Addition of at least 10% formamide to urea/polyacrylamide sequencing gels improves the d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64bac418f07d20b407560e283a7fe917
https://pubmed.ncbi.nlm.nih.gov/1451681
https://pubmed.ncbi.nlm.nih.gov/1451681
Publikováno v:
Journal of chromatography. 559(1-2)
A low cost, 0.75-mW helium neon laser, operating in the green region at 534.5 nm, is used to excite fluorescence from tetramethylrhodamine isothiocyanate-labelled DNA fragments that have been separated by capillary gel electrophoresis.The detection l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e101e9bc3e5639a0015500dc68e9bc35
https://pubmed.ncbi.nlm.nih.gov/1761625
https://pubmed.ncbi.nlm.nih.gov/1761625
Publikováno v:
SPIE Proceedings.
Low zeptomole (1 zmol equals 10-21 mol = 600 molecules) detection limits are produced for DNA sequencing by capillary gel electrophoresis. A 750 (mu) w green helium-neon laser ((lambda) equals 543.5 nm) is used to excite tetramethylrhodamine-labeled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46b32f1b8dc09787ce383bdf16ebebf5
https://doi.org/10.1117/12.44240
https://doi.org/10.1117/12.44240