Modulated efficacy CRISPRi reveals evolutionary conservation of essential gene expression-fitness relationships in bacteria

Autor: Carol A. Gross, Todor H, Melanie R. Silvis, Marco Jost, Jason M. Peters, Koo B, Jonathan S. Weissman, Hawkins Js, Hearne Cc

Essential genes are the central hubs of cellular networks. Despite their importance, the lack of high-throughput methods for titrating their expression has limited our understanding of the fitness landscapes against which essential gene expression le

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9ff58116caa867f65f2803ae29b3b83

Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli.

Autor: Silvis MR; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Rajendram M; Department of Bioengineering, Stanford Universitygrid.168010.e, Stanford, California, USA., Shi H; Department of Microbiology and Immunology, Stanford Universitygrid.168010.e School of Medicine, Stanford, California, USA., Osadnik H; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Gray AN; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Cesar S; Department of Microbiology and Immunology, Stanford Universitygrid.168010.e School of Medicine, Stanford, California, USA., Peters JM; Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, USA., Hearne CC; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Kumar P; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Todor H; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA., Huang KC; Department of Bioengineering, Stanford Universitygrid.168010.e, Stanford, California, USA.; Department of Microbiology and Immunology, Stanford Universitygrid.168010.e School of Medicine, Stanford, California, USA.; Chan Zuckerberg Biohub, San Francisco, California, USA., Gross CA; Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA.

Publikováno v: MBio [mBio] 2021 Oct 26; Vol. 12 (5), pp. e0256121. Date of Electronic Publication: 2021 Oct 12.

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.

Autor: Peters JM; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. jason.peters@wisc.edu.; Pharmaceutical Sciences Division, and Departments of Bacteriology, and of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA. jason.peters@wisc.edu., Koo BM; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Patino R; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Heussler GE; Division of Biological Sciences, University of California, San Diego, San Diego, CA, USA.; Center for Microbiome Innovation, Jacobs School of Engineering, University of California, San Diego, San Diego, CA, USA., Hearne CC; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Qu J; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.; Department of Clinical Laboratory, The Third People's Hospital of Shenzhen, Shenzhen, China., Inclan YF; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Hawkins JS; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Lu CHS; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Silvis MR; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Harden MM; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Osadnik H; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Peters JE; Department of Microbiology, Cornell University, Ithaca, NY, USA., Engel JN; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Dutton RJ; Division of Biological Sciences, University of California, San Diego, San Diego, CA, USA.; Center for Microbiome Innovation, Jacobs School of Engineering, University of California, San Diego, San Diego, CA, USA., Grossman AD; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Gross CA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. cgrossucsf@gmail.com.; Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA. cgrossucsf@gmail.com.; California Institute of Quantitative Biology, University of California, San Francisco, San Francisco, CA, USA. cgrossucsf@gmail.com., Rosenberg OS; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. oren.rosenberg@ucsf.edu.; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA. oren.rosenberg@ucsf.edu.

Publikováno v: Nature microbiology [Nat Microbiol] 2019 Feb; Vol. 4 (2), pp. 244-250. Date of Electronic Publication: 2019 Jan 07.