Experimental characterization of de novo proteins and their unevolved random-sequence counterparts.

Autor: Heames B; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Buchel F; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic.; Department of Biochemistry, Charles University, Prague, Czech Republic., Aubel M; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Tretyachenko V; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic., Loginov D; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Novák P; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Lange A; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Bornberg-Bauer E; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany. ebb@wwu.de.; Department of Protein Evolution, MPI for Developmental Biology, Tübingen, Germany. ebb@wwu.de., Hlouchová K; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic. klara.hlouchova@natur.cuni.cz.; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic. klara.hlouchova@natur.cuni.cz.

Publikováno v: Nature ecology & evolution [Nat Ecol Evol] 2023 Apr; Vol. 7 (4), pp. 570-580. Date of Electronic Publication: 2023 Apr 06.

Dissecting how modular polyketide synthase ketoreductases interact with acyl carrier protein-attached substrates

Autor: Moretto, L, Vance, S, Heames, B, Broadhurst, RW

Interaction studies using fragments excised from the modular mycolactone polyketide synthase show that ketoreductase domains possess a generic binding site for acyl carrier protein domains and provide evidence that the pendant 5′-phosphopantetheine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b888063333de49fbc54944a28bac81

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis.

Autor: Emond S; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK. emond.stephane@gmail.com.; Evonetix Ltd, Coldhams Business Park, Norman Way, Cambridge, CB1 3LH, UK. emond.stephane@gmail.com., Petek M; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK., Kay EJ; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.; Cancer Research UK Beatson Institute, Glasgow, G61 1BD, UK., Heames B; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.; Institute for Evolution and Biodiversity, Westfälische Wilhelms-Universität, Hüfferstrasse 1, 48149, Münster, Germany., Devenish SRA; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.; Fluidic Analytics, The Paddocks Business Centre, Cherry Hinton Road, Cambridge, CB1 8DH, UK., Tokuriki N; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada., Hollfelder F; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK. fh111@cam.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2020 Jul 10; Vol. 11 (1), pp. 3469. Date of Electronic Publication: 2020 Jul 10.

Becoming a de novo gene.

Autor: Bornberg-Bauer E; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany. ebb@uni-muenster.de., Heames B; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany.

Publikováno v: Nature ecology & evolution [Nat Ecol Evol] 2019 Apr; Vol. 3 (4), pp. 524-525.

Random, de novo, and conserved proteins: How structure and disorder predictors perform differently.

Autor: Middendorf L; Institute for Evolution and Biodiversity, University of Muenster, Muenster, Germany., Eicholt LA; Institute for Evolution and Biodiversity, University of Muenster, Muenster, Germany.

Publikováno v: Proteins [Proteins] 2024 Jun; Vol. 92 (6), pp. 757-767. Date of Electronic Publication: 2024 Jan 16.