Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Hazen P. Babcock"'
Autor:
Hazen P. Babcock, Xiaowei Zhuang
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
Abstract The resolution of super-resolution microscopy based on single molecule localization is in part determined by the accuracy of the localization algorithm. In most published approaches to date this localization is done by fitting an analytical
Externí odkaz:
https://doaj.org/article/ce4b1a8eeb6d49c8a88dd5cdb798e36e
Autor:
Doory Kim, Thomas J Deerinck, Yaron M Sigal, Hazen P Babcock, Mark H Ellisman, Xiaowei Zhuang
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0124581 (2015)
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows th
Externí odkaz:
https://doaj.org/article/30119eff22924e9ab850a9c79d54592c
Autor:
Guisheng Zhong, Jiang He, Ruobo Zhou, Damaris Lorenzo, Hazen P Babcock, Vann Bennett, Xiaowei Zhuang
Publikováno v:
eLife, Vol 3 (2014)
Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this latt
Externí odkaz:
https://doaj.org/article/93e491f51f9a4d62b83197bc780ce54f
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Optimal analysis of single molecule localization microscopy (SMLM) data acquired with a scientific Complementary Metal-Oxide-Semiconductor (sCMOS) camera relies on statistical compensation for its pixel-dependent gain, offset and readout noise. In th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c81c477bfbe16ad6c6e50ed62d89a0b5
http://europepmc.org/articles/PMC6889274
http://europepmc.org/articles/PMC6889274
Autor:
Xiaowei Zhuang, Hazen P. Babcock
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
Scientific Reports
Scientific Reports
The resolution of super-resolution microscopy based on single molecule localization is in part determined by the accuracy of the localization algorithm. In most published approaches to date this localization is done by fitting an analytical function
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f85961481bb142f396f7aff112859fa7
https://doaj.org/article/ce4b1a8eeb6d49c8a88dd5cdb798e36e
https://doaj.org/article/ce4b1a8eeb6d49c8a88dd5cdb798e36e
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance In this work, we developed an imaging-based pooled-library CRISPR screening approach that provides readouts of both phenotype and genotype of individual cells by high-resolution, high-content imaging. This approach promises to substantia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::060e2d77620e83fd82d9eab613bc5b46
https://pubmed.ncbi.nlm.nih.gov/31085639
https://pubmed.ncbi.nlm.nih.gov/31085639
Autor:
Thanh-an Pham, Ann P. Wheeler, Bernd Rieger, Tomas Lukes, Ricardo Henriques, Guy M. Hagen, Raimund J. Ober, Daniel Sage, Jonas Ries, Jerry Chao, Thomas Pengo, Silvia Colabrese, Anna Archetti, Seamus Holden, Ramraj Velmurugan, Hazen P. Babcock, Jean-Baptiste Sibarita, Alex Herbert, Michael Unser, Anurag Agrawal
Publikováno v:
Nature Methods
Sage, D, Pham, T-A, Babcock, H, Lukes, T, Pengo, T, Chao, J, Velmurugan, R, Herbert, A, Agrawal, A, Colabrese, S, Wheeler, A, Archetti, A, Rieger, B, Ober, R, Hagen, G M, Sibarita, J-B, Ries, J, Henriques, R, Unser, M & Holden, S 2019, ' Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software ', Nature Methods, vol. 16, no. 5, pp. 387-395 . https://doi.org/10.1038/s41592-019-0364-4
Nature methods
Sage, D, Pham, T-A, Babcock, H, Lukes, T, Pengo, T, Chao, J, Velmurugan, R, Herbert, A, Agrawal, A, Colabrese, S, Wheeler, A, Archetti, A, Rieger, B, Ober, R, Hagen, G M, Sibarita, J-B, Ries, J, Henriques, R, Unser, M & Holden, S 2019, ' Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software ', Nature Methods, vol. 16, no. 5, pp. 387-395 . https://doi.org/10.1038/s41592-019-0364-4
Nature methods
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort we designed a competition to extensi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3aed507a306bdaf63f01ac732a7ed85
https://eprints.soton.ac.uk/430424/
https://eprints.soton.ac.uk/430424/
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
Scientific Reports
Scientific Reports
Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relative
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::740d5219eb05832ce49ae0ee7530c418
https://doi.org/10.1101/505784
https://doi.org/10.1101/505784
Autor:
Hazen P. Babcock, Fang Huang
Optimal analysis of single molecule localization microscopy (SMLM) data acquired with a CMOS camera requires compensation for single pixel differences in gain, offset and readout noise. For some CMOS cameras we found that it is also necessary to comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b80c7434d257111c9875219547a6d780
Autor:
Thanh-an Pham, Hazen P. Babcock, Ann P. Wheeler, Daniel Sage, Thomas Pengo, Guy M. Hagen, Jonas Ries, Jerry Chao, Alex Herbert, Anna Archetti, Silvia Colabrese, Anurag Agrawal, Tomas Lukes, Michael Unser, Jean-Baptiste Sibarita, Bernd Rieger, Raimund J. Ober, Velmurugan R, Seamus Holden, Ricardo Henriques
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for thei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3ad65b159ab703fda37dd6296388568