An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation

Autor: James E. Bear, Ian J. Davis, Hashem A. Meriesh, Gregory R. Keele, Austin J. Hepperla, Seth P. Zimmerman, David Restrepo, Brian Kuhlman, Hayretin Yumerefendi, Andrew M. Lerner, Brian D. Strahl

Publikováno v: Genome Res

Histone H3 lysine 36 methylation (H3K36me) is a conserved histone modification associated with transcription and DNA repair. Although the effects of H3K36 methylation have been studied, the genome-wide dynamics of H3K36me deposition and removal are n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8432249b53e73a13045dda8371f4204e
https://pubmed.ncbi.nlm.nih.gov/33020206

Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution

Autor: Odessa J Goudy, Brian D. Strahl, Andrew M. Lerner, Brian Kuhlman, Hayretin Yumerefendi

Publikováno v: ACS Synthetic Biology. 7:2898-2907

Optogenetic techniques use light-responsive proteins to study dynamic processes in living cells and organisms. These techniques typically rely on repurposed naturally occurring light-sensitive proteins to control sub-cellular localization and activit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76668a613f6f9ddc8afa689c45c73122
https://doi.org/10.1021/acssynbio.8b00368

Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites

Autor: Dmitri I. Svergun, Andrew A. McCarthy, Bertrand Simon, Carsten Schultz, Juha Vahokoski, Hayretin Yumerefendi, Petri Kursula, Haydyn D. T. Mertens, Jan Erik Hoffmann, Matthias Wilmanns, Dana Komadina, Anne-Sophie Huart, Darren J. Hart, Koen Temmerman

Publikováno v: Structure (London, England : 1993)
Structure (London, England : 1993), 2016, 24 (6), pp.851-61. ⟨10.1016/j.str.2016.03.020⟩
Structure(London, England:1993)

Summary The regulation of many protein kinases by binding to calcium/calmodulin connects two principal mechanisms in signaling processes: protein phosphorylation and responses to dose- and time-dependent calcium signals. We used the calcium/calmoduli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e91a1e3906af28b7a3ccd6ac9c47582

Light-Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch

Autor: Hui Wang, Andrew M. Lerner, Bob Goldstein, Brian Kuhlman, Per Malkus, Klaus M. Hahn, Daniel J. Dickinson, Hayretin Yumerefendi

Publikováno v: Chembiochem : a European journal of chemical biology. 19(12)

Cellular signal transduction is often regulated at multiple steps in order to achieve more complex logic or precise control of a pathway. For instance, some signaling mechanisms couple allosteric activation with localization to achieve high signal to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cdba7a9538b6da0c806d651479dc2c4
https://pubmed.ncbi.nlm.nih.gov/29446199

Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins

Autor: James E. Bear, Gurkan Guntas, Hayretin Yumerefendi, Ryan A. Hallett, Brian Kuhlman, Seth P. Zimmerman, Tishan Williams

Publikováno v: Proceedings of the National Academy of Sciences. 112:112-117

The discovery of light-inducible protein-protein interactions has allowed for the spatial and temporal control of a variety of biological processes. To be effective, a photodimerizer should have several characteristics: it should show a large change

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bee3381b1a782d1ccae98b4608d1568
https://doi.org/10.1073/pnas.1417910112