Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea

Autor: Loder, Andrew J., Han, Yejun, Hawkins, Aaron B., Lian, Hong, Lipscomb, Gina L., Schut, Gerrit J., Keller, Matthew W., Adams, Michael W.W., Kelly, Robert M.

Publikováno v: In Metabolic Engineering November 2016 38:446-463

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Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO 2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus.

Autor: Lian H; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Zeldes BM; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Lipscomb GL; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia., Hawkins AB; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Han Y; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Loder AJ; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Nishiyama D; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587., Adams MW; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia., Kelly RM; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27587. rmkelly@ncsu.edu.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2016 Dec; Vol. 113 (12), pp. 2652-2660. Date of Electronic Publication: 2016 Jun 30.