Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Hassan Motejadded"'
Publikováno v:
Applied Biochemistry and Biotechnology. 162:2098-2110
The presented work introduces a novel method to immobilize enzymes either purified or directly out of a crude extract onto magnetic particles in the micrometer range. This method is based on the creation of a fusion protein consisting of the enzyme o
Autor:
Hassan Motejadded, Daniela Siegmund, Heike Glauner, Peter Scheurich, Frank Henkler, Harald Wajant, Ottmar Janssen
Publikováno v:
European Journal of Biochemistry. 269:4819-4829
To gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (TRAF4) we analyzed GFP chimeras of full-length TRAF4 and various deletion mutants derived thereof. While TRAF4–GFP (T4–GFP) was clearly localized
Autor:
Hassan Motejadded, Josef Altenbuchner
Publikováno v:
Biotechnology letters. 31(4)
An E. coli vector system was constructed which allows the expression of fusion genes via a L: -rhamnose-inducible promotor. The corresponding fusion proteins consist of the maltose-binding protein and a His-tag sequence for affinity purification, the
Publikováno v:
Applied microbiology and biotechnology. 80(1)
The arylacetonitrilase of Pseudomonas fluorescens EBC191 catalyzes the conversion of (S)-mandelonitrile to (S)-mandelic acid and (S)-mandeloamide. This biotransformation is optimally performed under acidic pH values because (S)-mandelonitrile rapidly
Autor:
Heike, Glauner, Daniela, Siegmund, Hassan, Motejadded, Peter, Scheurich, Frank, Henkler, Ottmar, Janssen, Harald, Wajant
Publikováno v:
European journal of biochemistry. 269(19)
To gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (TRAF4) we analyzed GFP chimeras of full-length TRAF4 and various deletion mutants derived thereof. While TRAF4-GFP (T4-GFP) was clearly localized in
Publikováno v:
Biological chemistry. 380(12)
We cloned and successfully expressed the gene for xylitol dehydrogenase from Galactocandida mastotermitis in Escherichia coli. The amino acid sequence revealed that the enzyme belongs to the superfamily of zinc containing, medium-chain alcohol dehydr