Acetyl-CoA carboxylase inhibition increases retinal pigment epithelial cell fatty acid flux and restricts apolipoprotein efflux.

Autor: Hass DT; Department of Biochemistry, University of Washington, Seattle, Washington, USA. Electronic address: dhass@uw.edu., Pandey K; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Engel A; Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Hospital, Seattle, Washington, USA., Horton N; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Haydinger CD; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Robbings BM; Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA., Lim RR; Department of Ophthalmology, University of Washington, Seattle, Washington, USA., Sadilek M; Department of Chemistry, University of Washington, Seattle, Washington, USA., Zhang Q; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, USA., Gulette GA; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, USA., Li A; Department of Medicinal Chemistry, University of Washington, Seattle, Washington, USA., Xu L; Department of Medicinal Chemistry, University of Washington, Seattle, Washington, USA., Miller JML; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, USA., Chao JR; Department of Ophthalmology, University of Washington, Seattle, Washington, USA., Hurley JB; Department of Biochemistry, University of Washington, Seattle, Washington, USA; Department of Ophthalmology, University of Washington, Seattle, Washington, USA. Electronic address: jbhhh@uw.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2024 Oct; Vol. 300 (10), pp. 107772. Date of Electronic Publication: 2024 Sep 12.

CRISPR editing of anti-anemia drug target rescues independent preclinical models of retinitis pigmentosa.

Autor: Nolan ND; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA., Cui X; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA., Robbings BM; Department of Biochemistry, The University of Washington, Seattle, WA 98195, USA; Diabetes Institute, The University of Washington, Seattle, WA 98195, USA., Demirkol A; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA; Vocational School of Health Services, Uskudar University, 34672 Istanbul, Turkey., Pandey K; Department of Biochemistry, The University of Washington, Seattle, WA 98195, USA., Wu WH; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA., Hu HF; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA., Jenny LA; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA., Lin CS; Herbert Irving Comprehensive Cancer Center, Department of Pathology & Cell Biology, Columbia University Irving Medical Center, New York, NY 10032, USA; Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA., Hass DT; Department of Biochemistry, The University of Washington, Seattle, WA 98195, USA., Du J; Department of Ophthalmology and Visual Sciences, West Virginia University, Morgantown, WV 26506, USA; Department of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, WV 26501, USA., Hurley JB; Department of Biochemistry, The University of Washington, Seattle, WA 98195, USA. Electronic address: jbhhh@uw.edu., Tsang SH; Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY 10032, USA; Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA; Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY 10032, USA; Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA. Electronic address: sht2@columbia.edu.

Publikováno v: Cell reports. Medicine [Cell Rep Med] 2024 Apr 16; Vol. 5 (4), pp. 101459. Date of Electronic Publication: 2024 Mar 21.

Medium Depth Influences O2 Availability and Metabolism in Human RPE Cultures.

Autor: Hass DT; Department of Biochemistry, The University of Washington, Seattle, Washington, United States., Zhang Q; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, United States., Autterson GA; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, United States., Bryan RA; Lucid Scientific, Atlanta, Georgia, United States., Hurley JB; Department of Biochemistry, The University of Washington, Seattle, Washington, United States., Miller JML; Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, United States.; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, Michigan, United States.

Publikováno v: Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2023 Nov 01; Vol. 64 (14), pp. 4.

Medium depth influences O 2 availability and metabolism in cultured RPE cells.

Autor: Hass DT, Zhang Q, Autterson G, Bryan R, Hurley JB, Miller JM

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 Mar 19. Date of Electronic Publication: 2023 Mar 19.

Transducin-Deficient Rod Photoreceptors Evaluated With Optical Coherence Tomography and Oxygen Consumption Rate Energy Biomarkers.

Autor: Berkowitz BA; Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States., Podolsky RH; Biostatistics and Study Methodology, Children's National Hospital, Silver Spring, Maryland, United States., Childers KL; Beaumont Research Institute, Beaumont Health, Royal Oak, Michigan, United States., Roberts R; Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States., Katz R; Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States., Waseem R; Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States., Robbings BM; Department of Biochemistry, Department of Ophthalmology, University of Washington, Seattle, Washington, United States.; Department of Medicine, UW Medicine Diabetes Institute, University of Washington, Seattle, Washington, United States., Hass DT; Department of Biochemistry, Department of Ophthalmology, University of Washington, Seattle, Washington, United States., Hurley JB; Department of Biochemistry, Department of Ophthalmology, University of Washington, Seattle, Washington, United States., Sweet IR; Department of Medicine, UW Medicine Diabetes Institute, University of Washington, Seattle, Washington, United States., Goodman C; Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States., Qian H; Visual Function Core, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States., Alvisio B; OSIO Bioinformatics Core, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States., Heaps S; OSIO Bioinformatics Core, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.

Publikováno v: Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2022 Dec 01; Vol. 63 (13), pp. 22.