Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Hasmukh V. Patel"'
Autor:
Hasmukh V. Patel, Keith D. Garlid, Debra L. Murdza-Inglis, Martin Modrianský, Karl B. Freeman
Publikováno v:
The Journal of biological chemistry. 272(40)
Primary regulation of uncoupling protein is mediated by purine nucleotides, which bind to the protein and allosterically inhibit fatty acid-induced proton transport. To gain increased understanding of nucleotide regulation, we evaluated the role of b
Autor:
Zai-Chuan Wu, Bhag Singh, Bohdan J. Soltys, Karl B. Freeman, Hasmukh V. Patel, Radhey S. Gupta
Publikováno v:
Experimental cell research. 234(2)
The cDNA for Chinese hamster mitochondrial Hsp70 (mHsp70) was cloned and sequenced using a polymerase chain reaction probe based on conserved regions in the Hsp70 family of proteins. The encoded protein consists of 679 amino acids which includes a N-
Publikováno v:
Biochemical and biophysical research communications. 169(2)
The mitochondrial import of a member of the “chaperonin” group of proteins which play an essential role in the import of protein into organelles and their subsequent proper folding has been examined. The cDNA for human hsp60 (synonyms: GroEL homo
Publikováno v:
Biochemistry and Cell Biology. 65:955-959
The time course of changes in the level of uncoupling protein mRNA when cold-acclimated mice were returned to a thermoneutral environment (33 °C) was examined using a cDNA probe. Upon deacclimation, there was a marked loss of uncoupling protein mRNA
Publikováno v:
Biochemistry and Cell Biology. 67:147-151
The effect of temperature on the amount of uncoupling protein mRNA in rat brown adipose tissue was examined after 1 and 14 days of exposure to cold. The relative amounts after 1 day, compared with rats kept at a thermoneutral temperature of 28 °C, w
Autor:
Kimberley A. Olynyk, Shuen-Kuei Liao, Karl B. Freeman, Hasmukh V. Patel, Michael Afong, John Arnold
Publikováno v:
Canadian Journal of Biochemistry and Cell Biology. 63:96-101
The immunological relationship of the uncoupling protein from brown adipose tissue of several mammalian species was examined by using a rabbit antibody preparation against the rat protein. Complete cross-reactivity of the antibody to the protein from
Publikováno v:
Canadian Journal of Biochemistry. 57:662-665
The binding of 14C-labelled bovine and porcine malate dehydrogenase (EC 1.1.1.37) to rat liver mitochondria and mitoplasts was examined. The bovine enzyme was found to associate nonspecifically with isolated mitochondria and sonicated mitoplasts. Sca
Autor:
Hasmukh V. Patel, Craig L.J. Parfett, Kimberly A. Olynyk, Susanna Reichling, Karl B. Freeman, Robert G. Ridley
Publikováno v:
Bioscience Reports. 6:87-94
Poly(A)+ RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322, Immunological screening of 1,500 colonies with an affinity-purified antise
Publikováno v:
FEBS Letters. 133:127-129
Publikováno v:
Somatic cell genetics. 7(1)
A clone of Chinese hamster ovary (CHO) cells, BT3, resistant to Tevenel, the sulfamoyl analog of chloramphenicol has been isolated. Resistance was found to be at the mitochondrial level and was shown to be cytoplasmically inherited. This marker was t