Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Haruko Maeda"'
Publikováno v:
Applied Microbiology and Biotechnology. 72:1229-1237
The display of a protease, carboxypeptidase Y (CPY) or procarboxypeptidase Y (proCPY), which is the vacuolar protease, on the yeast-cell surface was successfully performed using yeast-cell-surface engineering for the first time. Through that we could
Publikováno v:
Applied Microbiology and Biotechnology. 69:423-427
We constructed a novel protein-purification system in which Saccharomyces cerevisiae with a protein displayed on the cell surface is harvested and the displayed protein is then cleaved from the cell surface. GFPuv was used as a model protein in this
Autor:
Haruko Maeda, Eri Chatani, Rikimaru Hayashi, Takahiro Koyama, Hirohisa Izumi, Masatoshi Sugiura
Publikováno v:
Carbohydrate Research. 339:1041-1045
To obtain large amounts of deglycosylated procarboxypeptidase Y (proCPY), in which all of the N-glycosylation sites were replaced by alanine residue by the point mutation method, an expression system was constructed using Pichia pastoris. The secrete
Publikováno v:
food preservation science. 29:25-31
Bacillus sp. 11-4 metalloprotease (protease I), which shows collagenase-like specificity, was purified from the culture supernatant of the strain. The N-terminal amino acid sequence of the protease I was determined up to 43 residues from its N-termin
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 76:72-75
Although Streptococcus salivarius is one of the normal flora in the oral cavity and gastrointestinal tract, the agent may cause bacteremia, meningitis, endocarditis and sinusitis under certain circumstances. We report a 3-year-old female with meningi
Autor:
Kenichiro Kobayashi, Haruko Maeda, Toshikazu Nishio, Tsunekazu Haruta, Masaru Kubota, Shigekazu Kuroki, Gen Shinoda
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 75:981-988
We report an 8-year-old boy with acute focal bacterial nephritis (AFBN). At the age of 3 months, he had a history of urinary tract infection and vesicoureteral reflux. He was admitted to our hospital because of high fever and costovertebral angle pai
Publikováno v:
food preservation science. 26:308-313
Bacillus sp. 11-4 isolated from Vietnamese fish sauce was slightly halophilic and produced some extracellular protease. The proteases from Bacillus sp. 11-4 were purified by ion-exchange chromatography and gel filtration. The major extracellular prot
Publikováno v:
food preservation science. 26:263-271
We isolated protease-productive bacteria from fish sauces collected in Vietnam and investigated their characteristics. Bacteria were detected from all the fish sauces on the low saline agar medium including 2.5% sodium chloride, and viable cell count
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 73:753-755
A novel purification system for inactive precursor proteins without conventional time-consuming purification steps was established. We purified an inactive precursor protein, procarboxypeptidase Y (proCPY), which was displayed on the cell surface of
Publikováno v:
The Journal of Immunology. 162:807-812
IFN-γ exerts multiple biological activities in the modulation of immune responses by the induction of transcription factors. One transcriptional factor of the IFN regulatory factor family found to be critical in regulating IL-12-dependent IFN-γ pro