Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Haruhiro Muratsubaki"'
Publikováno v:
Cell Biochemistry and Function. 26:275-278
Mitochondrial respiration is inhibited in cells exposed to hypoxia, and the oxidation of NADH to NAD+ is blocked. As a result, oxidation reactions requiring NAD+ are blocked, disrupting cellular metabolism. We studied the influence of methylene blue,
Publikováno v:
Analytical Biochemistry. 359:161-166
A new enzymatic assay for specifically measuring allantoin concentration in serum has been developed. The currently used methods for allantoin analysis are time consuming and nonspecific or depend on the use of expensive equipment. In our method, all
Publikováno v:
Archives of Physiology and Biochemistry. 111:449-454
Exposure of sated rats to 45% N2 in air for 5h increased serum triglyceride levels by 212% over the levels in normoxic rats. This increase in triglyceride levels was accompanied by a decrease in plasma triglyceride hydrolase activity after intravenou
Publikováno v:
FEMS Microbiology Letters. 215:103-108
In Saccharomyces cerevisiae, there are two isoenzymes of fumarate reductase (FRDS1 and FRDS2), encoded by the FRDS and OSM1 genes, respectively. Simultaneous disruption of these two genes results in a growth defect of the yeast under anaerobic condit
Publikováno v:
DNA Research. 3:263-267
A gene of the soluble fumarate reductase (FRDS) that binds FAD non-covalently was cloned by polymerase chain reaction (PCR) using degenerate oligonucleotides designed from partial amino acid sequences of highly purified enzyme. The nucleotide sequenc
Publikováno v:
Preparative Biochemistry. 24:289-296
The rapid and effective purification of soluble fumarate reductase from baker's yeast achieved by Blue Sepharose CL–6B chromatography. Cibacron Blue F3GA, the chromophore of Blue Sepharose, inhibited the activity of fumarate reductase. The enzyme b
Autor:
Akiko Yamaki, Haruhiro Muratsubaki
Publikováno v:
Indian journal of clinical biochemistry : IJCB. 26(4)
The effect of acute hypoxic hypoxia on the profile of plasma amino acids in rats was studied and compared to that resulting from acute liver injury induced by giving carbon tetrachloride. In hypoxic rats exposed to 45% air in N(2) for 5 h, the concen
Publikováno v:
Analytical biochemistry. 378(1)
Current conventional measurement of allantoin levels in human serum uses an HPLC method. However, performing this assay is time-consuming and sample-intensive, and it requires expensive equipment. We have developed a novel enzyme cycling method for m
Publikováno v:
Analytical biochemistry. 307(2)
Analysis of serum proteins by native polyacrylamide gel electrophoresis is difficult because albumin is abundant in serum and interferes with the resolution of other proteins, especially α-antitrypsin which has mobility that is very similar to that
Publikováno v:
FEMS microbiology letters. 165(1)
In Saccharomyces cerevisiae, the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneo