A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Autor: Trevor Y. H. Ho, Alexander Shao, Zeyu Lu, Harri Savilahti, Filippo Menolascina, Lei Wang, Neil Dalchau, Baojun Wang

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

Split inteins are powerful tools for designing synthetic split proteins. Here the authors use a mini-Mu transposon screen to map split sites, enabling the development of protein-based logic gates and fine control of protein activity.

Externí odkaz: https://doaj.org/article/4b896aa452b543a29fce64b160991170

Flexibility in MuA transposase family protein structures: functional mapping with scanning mutagenesis and sequence alignment of protein homologues.

Autor: Tiina S Rasila, Mauno Vihinen, Lars Paulin, Saija Haapa-Paananen, Harri Savilahti

Publikováno v: PLoS ONE, Vol 7, Iss 5, p e37922 (2012)

MuA transposase protein is a member of the retroviral integrase superfamily (RISF). It catalyzes DNA cleavage and joining reactions via an initial assembly and subsequent structural transitions of a protein-DNA complex, known as the Mu transpososome,

Externí odkaz: https://doaj.org/article/d5d2a6fd8b7347a3b833293492b866c2

The C-terminal region of phytoene synthase is a key element to control carotenoid biosynthesis in the haloarchaeon Haloferax volcanii

Autor: Micaela Cerletti, Agustín Rabino, Roberto A. Paggi, Celeste Ferrari, Ansgar Poetsch, Harri Savilahti, Saija Kiljunen, Rosana E. De Castro

Publikováno v: The Biochemical journal. 479(22)

Phytoene synthase (PSY) converts two molecules of geranyl-geranyl diphosphate to phytoene, the key regulatory step in carotenogenesis. However, post-translational mechanisms that control PSY expression are scarcely understood. Carotenoid biosynthesis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd7f0241b8739efac6fbc2368e75ac27
https://pubmed.ncbi.nlm.nih.gov/36373632

A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Autor: Lei Wang, Alexander Shao, Trevor Y. H. Ho, Filippo Menolascina, Zeyu Lu, Harri Savilahti, Neil Dalchau, Baojun Wang

Publikováno v: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Ho, T, Shao, A, Lu, Z, Savilahti, H, Menolascina, F, Wang, L, Dalchau, N & Wang, B 2021, ' A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction ', Nature Communications, vol. 12, 2200 . https://doi.org/10.1038/s41467-021-22404-9

Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f02d40cbb3979058ddf2098ee3785be
https://pubmed.ncbi.nlm.nih.gov/33850130

Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Autor: Swathi Dantuluri, Sungmin Hwang, Zyan Gani, Saija Kiljunen, Julie A. Maupin-Furlow, Miguel Gomez, Whinkie Leung, Alexander N Pillai, Harri Savilahti, Lana J. McMillan

Publikováno v: Genes
Genes, Vol 9, Iss 11, p 562 (2018)
Volume 9
Issue 11

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9228833f2460ba9c90b0bc44d45e3da
http://europepmc.org/articles/PMC6267482