Zobrazeno 1 - 10
of 113
pro vyhledávání: '"Harri Härmä"'
Autor:
Candy Laura Steffen, Ganesh babu Manoharan, Karolina Pavic, Alejandro Yeste-Vázquez, Matias Knuuttila, Neha Arora, Yong Zhou, Harri Härmä, Anthoula Gaigneaux, Tom N. Grossmann, Daniel Kwaku Abankwa
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-15 (2024)
Abstract Hyperactive Ras signalling is found in most cancers. Ras proteins are only active in membrane nanoclusters, which are therefore potential drug targets. We previously showed that the nanocluster scaffold galectin-1 (Gal1) enhances H-Ras nanoc
Externí odkaz:
https://doaj.org/article/824fba21fa7f4eef9a59a3a33f13cb35
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract Thermal shift assay (TSA) with altered temperature has been the most widely used method for monitoring protein stability for drug research. However, there is a pressing need for isothermal techniques as alternatives. This urgent demand arise
Externí odkaz:
https://doaj.org/article/7599890d644e4e2badd1628ca8a580e5
Publikováno v:
ACS Omega, Vol 4, Iss 15, Pp 16501-16507 (2019)
Externí odkaz:
https://doaj.org/article/29170cabf2e942eebfff265169475677
Publikováno v:
ACS Omega, Vol 4, Iss 2, Pp 4269-4275 (2019)
Externí odkaz:
https://doaj.org/article/d068f18412194e6399e98b0df6ee323e
Autor:
Kari Kopra, Salla Valtonen, Randa Mahran, Jonas N. Kapp, Nazia Hassan, William Gillette, Bryce Dennis, Lianbo Li, Kenneth D. Westover, Andreas Plückthun, Harri Härmä
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 13, p 7095 (2022)
Thermal unfolding methods are commonly used as a predictive technique by tracking the protein’s physical properties. Inherent protein thermal stability and unfolding profiles of biotherapeutics can help to screen or study potential drugs and to fin
Externí odkaz:
https://doaj.org/article/55616800df26442e83ba885b4eee505a
Autor:
Salla Valtonen, Emmiliisa Vuorinen, Ville Eskonen, Morteza Malakoutikhah, Kari Kopra, Harri Härmä
Publikováno v:
mAbs, Vol 13, Iss 1 (2021)
Protein aggregation is a spontaneous process affected by multiple external and internal properties, such as buffer composition and storage temperature. Aggregation of protein-based drugs can endanger patient safety due, for example, to increased immu
Externí odkaz:
https://doaj.org/article/3faa2234daa44908bdbbbd5ac19c4d0b
Autor:
Emmiliisa Vuorinen, Salla Valtonen, Nazia Hassan, Randa Mahran, Huda Habib, Morteza Malakoutikhah, Kari Kopra, Harri Härmä
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 12, p 6362 (2021)
Proteases are a group of enzymes with a catalytic function to hydrolyze peptide bonds of proteins. Proteases regulate the activity, signaling mechanism, fate, and localization of many proteins, and their dysregulation is associated with various patho
Externí odkaz:
https://doaj.org/article/a4df3643f2114799b56022f107f47cfe
Autor:
Päivi Grönroos, Nur-E-Habiba, Kalle Salminen, Marja Nissinen, Tomi Tuomaala, Kim Miikki, Qiang Zhang, Nan Wei, Esko Kauppinen, Jarkko Eskola, Harri Härmä, Sakari Kulmala
Publikováno v:
Sensors, Vol 19, Iss 12, p 2751 (2019)
Novel hot electron-emitting working electrodes and conventional counter electrodes were created by screen printing. Thus, low-cost disposable electrode chips for bioaffinity assays were produced to replace our older expensive electrode chips manufact
Externí odkaz:
https://doaj.org/article/140f78ae2d174b4d8da329b0f57b4eb0
Autor:
Jouko Peltonen, Ronald Österbacka, Marjo Yliperttula, Jawad Sarfraz, Harri Härmä, Anni Määttänen, Tuomas Närjeoja, Tapani Viitala, Björn Törngren, Himadri Majumdar, Petri Ihalainen
Publikováno v:
Biosensors, Vol 3, Iss 1, Pp 1-17 (2012)
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.
Externí odkaz:
https://doaj.org/article/fa0d03636f7b4b73a595c4c29d7ee11f
Publikováno v:
Sensors, Vol 11, Iss 12, Pp 11335-11342 (2011)
Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, t
Externí odkaz:
https://doaj.org/article/01be7cfeac2e4599a93100bcce0945fa