Zobrazeno 1 - 10
of 99
pro vyhledávání: '"Harriëtte Oldenhof"'
Autor:
Sercan Içli, Meisam Soleimani, Harriëtte Oldenhof, Harald Sieme, Peter Wriggers, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes agains
Externí odkaz:
https://doaj.org/article/42ec7603b6d742699dfadd8d7f2b2fb8
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin remains intact. In this study, we investigated drying- and temperature-induced conformational changes of nucleic acids and stallion sperm chroma
Externí odkaz:
https://doaj.org/article/3799dd8fb9e44ecf930c4cf73237f882
Autor:
Dejia Liu, Sükrü Caliskan, Bita Rashidfarokhi, Harriëtte Oldenhof, Klaus Jung, Harald Sieme, Andres Hilfiker, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Freeze-drying can be used to ensure off-the-shelf availability of decellularized heart valves for cardiovascular surgery. In this study, decellularized porcine aortic heart valves were analyzed by nitroblue tetrazolium (NBT) staining and Fou
Externí odkaz:
https://doaj.org/article/c8342ed5b18f41329cfb21e52d1a6688
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Abstract Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are su
Externí odkaz:
https://doaj.org/article/24d31be547c8401abb3f6c4fde07df31
Autor:
Raffaele Brogna, Harriëtte Oldenhof, Harald Sieme, Constança Figueiredo, Tobias Kerrinnes, Willem F Wolkers
Publikováno v:
PLoS ONE, Vol 15, Iss 6, p e0234502 (2020)
Preservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry pr
Externí odkaz:
https://doaj.org/article/66af227af24345f9b9a75c1f9e2a42d9
Autor:
Miao Zhang, Harriëtte Oldenhof, Bulat Sydykov, Judith Bigalk, Harald Sieme, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Abstract The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a m
Externí odkaz:
https://doaj.org/article/6818fc6151594e72a6ebc0ad9c7eefd8
Publikováno v:
PLoS ONE, Vol 13, Iss 7, p e0199867 (2018)
Cryopreservation of biological materials is predominantly done using liquid nitrogen, and its application involves high maintenance costs and the need for periodical refilling of liquid nitrogen. Stable storage in mechanical freezers at -80°C would
Externí odkaz:
https://doaj.org/article/62065f92f85644df8f1663ad75a25707
Autor:
Harriëtte Oldenhof, Huaqing Yang, Xing Luo, Willem F. Wolkers, David Pruß, Harald Sieme, Dejia Liu, Jan Hegermann
Publikováno v:
Cryobiology. 101:67-77
Stallion sperm is typically cryopreserved using low cooling rates and low concentrations of cryoprotective agents (CPAs). The inevitable water-to-ice phase transition during cryopreservation is damaging and can be prevented using vitrification. Vitri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20992f5b1364ec8ad3296beb4309b927
https://doi.org/10.1016/j.cryobiol.2021.05.007
https://doi.org/10.1016/j.cryobiol.2021.05.007
Publikováno v:
Biochimica et biophysica acta. General subjects. 1867(1)
In this study we investigated hydrogen bonding interactions in hydrated and frozen solutions of different cryoprotective agents (CPAs) including dimethyl sulfoxide, glycerol, ethylene glycol, propylene glycol, and trehalose. We also investigated the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ef6da928f49caf7b72c4bd480bc7b3c
https://pubmed.ncbi.nlm.nih.gov/36243203
https://pubmed.ncbi.nlm.nih.gov/36243203
Autor:
Jiale, Han, Bulat, Sydykov, Huaqing, Yang, Harald, Sieme, Harriëtte, Oldenhof, Willem F, Wolkers
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
Scientific Reports
There is an increasing demand for female fertility preservation. Cryopreservation of ovarian cortex tissue by means of vitrification can be done ad-hoc and for pre-pubertal individuals. Obtaining a homogeneous distribution of protective agents in tis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::5c34bc54ca9cfe50a60849537db20e11
http://link.springer.com/article/10.1038/s41598-019-51903-5
http://link.springer.com/article/10.1038/s41598-019-51903-5