A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Autor: Trevor Y. H. Ho, Alexander Shao, Zeyu Lu, Harri Savilahti, Filippo Menolascina, Lei Wang, Neil Dalchau, Baojun Wang

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

Split inteins are powerful tools for designing synthetic split proteins. Here the authors use a mini-Mu transposon screen to map split sites, enabling the development of protein-based logic gates and fine control of protein activity.

Externí odkaz: https://doaj.org/article/4b896aa452b543a29fce64b160991170

Intein-assisted bisection mapping systematically splits proteins for Boolean logic and inducibility engineering

Autor: Baojun Wang, Lu Z, Wang L, Filippo Menolascina, Anwen Shao, Ho Tyh, Harri Savilahti, Neil Dalchau

Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ad34f080f8e6a1888c1b0548efa0db99
https://doi.org/10.1101/2020.11.30.381921

A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Autor: Lei Wang, Alexander Shao, Trevor Y. H. Ho, Filippo Menolascina, Zeyu Lu, Harri Savilahti, Neil Dalchau, Baojun Wang

Publikováno v: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Ho, T, Shao, A, Lu, Z, Savilahti, H, Menolascina, F, Wang, L, Dalchau, N & Wang, B 2021, ' A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction ', Nature Communications, vol. 12, 2200 . https://doi.org/10.1038/s41467-021-22404-9

Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f02d40cbb3979058ddf2098ee3785be
https://pubmed.ncbi.nlm.nih.gov/33850130

Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice.

Autor: Hilkka Turakainen, Jonna Saarimäki-Vire, Natalia Sinjushina, Juha Partanen, Harri Savilahti

Publikováno v: PLoS ONE, Vol 4, Iss 2, p e4341 (2009)

Conditional gene targeting strategies are progressively used to study gene function tissue-specifically and/or at a defined time period. Instrumental to all of these strategies is the generation of targeting vectors, and any methodology that would st

Externí odkaz: https://doaj.org/article/50a05cc29cc148708d4935af03599ad2

Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Autor: Swathi Dantuluri, Sungmin Hwang, Zyan Gani, Saija Kiljunen, Julie A. Maupin-Furlow, Miguel Gomez, Whinkie Leung, Alexander N Pillai, Harri Savilahti, Lana J. McMillan

Publikováno v: Genes
Genes, Vol 9, Iss 11, p 562 (2018)
Volume 9
Issue 11

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9228833f2460ba9c90b0bc44d45e3da
http://europepmc.org/articles/PMC6267482