Zobrazeno 1 - 10
of 485
pro vyhledávání: '"Harold G. Craighead"'
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-13 (2023)
Abstract Viral vectors represent a bottleneck in the manufacturing of cellular therapies. Electroporation has emerged as an approach for non-viral transfection of primary cells, but standard cuvette-based approaches suffer from low throughput, diffic
Externí odkaz:
https://doaj.org/article/d90ef26d140643a9a9f2c0187c1ecea3
Autor:
Harold G. Craighead, Christine P. Tan
Publikováno v:
Materials, Vol 3, Iss 3, Pp 1803-1832 (2010)
Parylene is a family of chemically vapour deposited polymer with material properties that are attractive for biomedicine and nanobiotechnology. Chemically inert parylene “peel-off” stencils have been demonstrated for micropatterning biomolecular
Externí odkaz:
https://doaj.org/article/cf79504e5b1141acb396a6927826acb9
Autor:
Hsien-Wei Meng, John M Pagano, Brian S White, Yoshiko Toyoda, Irene M Min, Harold G Craighead, David Shalloway, John T Lis, Kai Xiao, Moonsoo M Jin
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e93052 (2014)
SELEX, the process of selecting aptamers, is often hampered by the difficulty of preparing target molecules in their native forms and by a lack of a simple yet quantitative assay for monitoring enrichment and affinity of reactive aptamers. In this st
Externí odkaz:
https://doaj.org/article/2aa6810a600f40d3b76d3ad596d8c773
Autor:
Roberto De Alba, Isaac R. Storch, Jeevak M. Parpia, T. S. Abhilash, Vivekananda P. Adiga, Robert A. Barton, Paul L. McEuen, Harold G. Craighead
Publikováno v:
Storch, I R, De Alba, R, Adiga, V P, Abhilash, T S, Barton, R A, Craighead, H G, Parpia, J M & McEuen, P L 2018, ' Young's modulus and thermal expansion of tensioned graphene membranes ', Physical Review B, vol. 98, no. 8, 085408 . https://doi.org/10.1103/PhysRevB.98.085408
Tensioned graphene membranes are of interest both for fundamental physics and for applications ranging from water filtration to nanomechanical resonators. It is generally assumed that these membranes have a stretching modulus of about 340 N/m and a n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f9954fb00166fee0f8c47893d92d290
https://cris.vtt.fi/en/publications/98432b45-c997-4d61-95ac-7773693e7d69
https://cris.vtt.fi/en/publications/98432b45-c997-4d61-95ac-7773693e7d69
Autor:
Harold G. Craighead, Sarah J. Reinholt
Publikováno v:
Analytical chemistry. 90(4)
We present a microfluidic device for specifically capturing cancer cells and isolating their genomic DNA (gDNA) for specific amplification and sequence analysis. To capture cancer cells within the device, nucleic acid aptamers that specifically bind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bddffb4e47599002d23fcc2451943241
https://pubmed.ncbi.nlm.nih.gov/29323871
https://pubmed.ncbi.nlm.nih.gov/29323871
Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias
Publikováno v:
PLoS ONE
PLoS ONE, Vol 13, Iss 2, p e0191520 (2018)
PLoS ONE, Vol 13, Iss 2, p e0191520 (2018)
Single cell whole genome amplification is susceptible to amplification biases that impact the accuracy of single cell sequencing data. To address this, we have developed a microfluidic device for the isolation and purification of genomic DNA from ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5344dc7304077555d42c023fe401a44b
https://pubmed.ncbi.nlm.nih.gov/29432426
https://pubmed.ncbi.nlm.nih.gov/29432426
Publikováno v:
ACS Nano
The organization and dynamics of plasma membrane components at the nanometer scale are essential for biological functions such as transmembrane signaling and endocytosis. Planarized nanoscale apertures in a metallic film are demonstrated as a means o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::820883e276f1a51a25d5231b9b8cf76c
https://doi.org/10.1021/nn502593k
https://doi.org/10.1021/nn502593k
Autor:
Carlos A. Aguilar, Harold G. Craighead
Publikováno v:
Nature Nanotechnology. 8:709-718
This Review examines recent advances in the use of micro- and nanoscale devices for studying epigenetic modifications, including covalent DNA modifications, differences in histone proteins and higher-order chromatin structures. Deoxyribonucleic acid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052cf358ef69d0618d1779999712f953
https://doi.org/10.1038/nnano.2013.195
https://doi.org/10.1038/nnano.2013.195
Autor:
Jaime J. Benítez, James A. Hagarman, Chan Yang Ju, Benjamin Cipriany, Kylan Szeto, Paul D. Soloway, Harold G. Craighead, Christopher B. Wallin, Patrick J. Murphy
Publikováno v:
Proceedings of the National Academy of Sciences. 110:7772-7777
Proper placement of epigenetic marks on DNA and histones is fundamental to normal development, and perturbations contribute to a variety of disease states. Combinations of marks act together to control gene expression; therefore, detecting their colo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ee79095f0f42aca9d5367f110a8c1e9
https://doi.org/10.1073/pnas.1218495110
https://doi.org/10.1073/pnas.1218495110
Autor:
Thomas Alava, Jason A. Mann, Jeevak M. Parpia, Cécile Théodore, Harold G. Craighead, Jaime J. Benítez, William R. Dichtel
Publikováno v:
Analytical Chemistry. 85:2754-2759
Graphene's suite of useful properties makes it of interest for use in biosensors. However, graphene interacts strongly with hydrophobic components of biomolecules, potentially altering their conformation and disrupting their biological activity. We h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5297b5b9f5c6e412c8ea3e141254116c
https://doi.org/10.1021/ac303268z
https://doi.org/10.1021/ac303268z