Potentiometrische Messungen der Hautoberflächenazidität hautgesunder Kinder und von Kindern mit Mukoviszidose

Autor: Kestel Fg, Harms Dw

Publikováno v: Klinische Pädiatrie. 195:330-333

Actual acidity of the skin surface as well as the capacity to neutralize topically applied 0,01 n NaOH were measured by means of a potentiometric procedure. 50 children without diseases of their skin showed pH values between 4,5 and 6,1 (mean pH = 5,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c1c4f986cd8cada7780b0ca3c6d15af8
https://doi.org/10.1055/s-2008-1034391

Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".

Autor: Gurumurthy CB; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.; Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu., O'Brien AR; Transformational Bioinformatics, Health and Biosecurity Business Unit, CSIRO, Sydney, Australia.; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Quadros RM; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA., Adams J Jr; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Alcaide P; Department of Immunology, Tufts University School of Medicine, Boston, USA., Ayabe S; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Ballard J; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Batra SK; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Beauchamp MC; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Becker KA; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Bernas G; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Brough D; Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK., Carrillo-Salinas F; Department of Immunology, Tufts University School of Medicine, Boston, USA., Chan W; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Chen H; School of Medicine, Indiana University, Indianapolis, IN, 46202, USA., Dawson R; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., DeMambro V; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., D'Hont J; Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Dibb K; Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK., Eudy JD; High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA., Gan L; University of Rochester Medical Center, Rochester, NY, 14642, USA., Gao J; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Gonzales A; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Guntur A; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Guo H; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Harms DW; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA., Harrington A; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Hentges KE; Division of Evolution and Genomic Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK., Humphreys N; Transgenic Unit core facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Imai S; Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Ishii H; Department of Medical Data Science, Osaka University Graduate School of Medicine, Suita, Japan., Iwama M; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Jonasch E; The University of Texas, MD Anderson Cancer Center, Houston, TX, USA., Karolak M; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Keavney B; Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester AND Manchester Heart Centre, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK., Khin NC; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Konno M; Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Osaka, Japan., Kotani Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Kunihiro Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Lakshmanan I; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Larochelle C; Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Lawrence CB; Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK., Li L; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Lindner V; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Liu XD; The University of Texas, MD Anderson Cancer Center, Houston, TX, USA., Lopez-Castejon G; Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK., Loudon A; Centre for Biological Timing, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Lowe J; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Jerome-Majeweska L; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Matsusaka T; Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Miura H; Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Miyasaka Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Morpurgo B; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Motyl K; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Nabeshima YI; Laboratory of Molecular Life Science, Foundation for Biomedical Research and Innovation, Kobe, Japan., Nakade K; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Nakashiba T; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Nakashima K; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Obata Y; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Ogiwara S; Department of Laboratory Animal Science, Support Center for Medical Research and Education, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Ouellet M; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Oxburgh L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Piltz S; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., Pinz I; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Ponnusamy MP; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Ray D; Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, OX37LE, UK., Redder RJ; High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA., Rosen CJ; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Ross N; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Ruhe MT; Mouse Biology Program, University of California, Davis, USA., Ryzhova L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Salvador AM; Department of Immunology, Tufts University School of Medicine, Boston, USA., Alam SS; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Sedlacek R; Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic., Sharma K; College of Osteopathic Medicine, Marian University, Indianapolis, IN, 46222, USA., Smith C; The University of Texas, MD Anderson Cancer Center, Houston, TX, USA., Staes K; Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Starrs L; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia., Sugiyama F; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Takahashi S; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Tanaka T; Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan., Trafford A; Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK., Uno Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Vanhoutte L; Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Vanrockeghem F; Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Willis BJ; Mouse Biology Program, University of California, Davis, USA., Wright CS; Department of Physical Therapy, School of Health and Human Sciences, Indiana University, Indianapolis, IN, 46202, USA., Yamauchi Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Yi X; Department of Physical Therapy, School of Health and Human Sciences, Indiana University, Indianapolis, IN, 46202, USA., Yoshimi K; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Zhang X; The University of Texas, MD Anderson Cancer Center, Houston, TX, USA., Zhang Y; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Ohtsuka M; Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Das S; Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, MN, USA., Garry DJ; Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, MN, USA.; Paul and Sheila Wellstone Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN, USA., Hochepied T; Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Thomas P; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., Parker-Thornburg J; The University of Texas, MD Anderson Cancer Center, Houston, TX, USA., Adamson AD; Transgenic Unit core facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Yoshiki A; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Schmouth JF; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Golovko A; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Thompson WR; Department of Physical Therapy, School of Health and Human Sciences, Indiana University, Indianapolis, IN, 46202, USA., Lloyd KCK; Mouse Biology Program, University of California, Davis, USA.; Department of Surgery, School of Medicine, University of California, Davis, Davis, USA., Wood JA; Mouse Biology Program, University of California, Davis, USA., Cowan M; McGill Integrated Core for Animal Modeling (MICAM), Montreal, Canada., Mashimo T; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan., Mizuno S; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Zhu H; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Kasparek P; Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic., Liaw L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Miano JM; University of Rochester Medical Center, Rochester, NY, 14642, USA., Burgio G; Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia. gaetan.burgio@anu.edu.au.

Publikováno v: Genome biology [Genome Biol] 2021 Apr 07; Vol. 22 (1), pp. 99. Date of Electronic Publication: 2021 Apr 07.

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.

Autor: Gurumurthy CB; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.; Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu., O'Brien AR; Transformational Bioinformatics, Health and Biosecurity Business Unit, CSIRO, North Ryde, Australia.; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Quadros RM; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA., Adams J Jr; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Alcaide P; Department of Immunology, Tufts University School of Medicine, Boston, USA., Ayabe S; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Ballard J; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Batra SK; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Beauchamp MC; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Becker KA; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Bernas G; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Brough D; Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK., Carrillo-Salinas F; Department of Immunology, Tufts University School of Medicine, Boston, USA., Chan W; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Chen H; School of Medicine, Indiana University, Indianapolis, IN, 46202, USA., Dawson R; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., DeMambro V; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., D'Hont J; Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Dibb KM; Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK., Eudy JD; High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA., Gan L; University of Rochester Medical Center, Rochester, NY, 14642, USA., Gao J; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Gonzales A; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Guntur AR; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Guo H; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Harms DW; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA., Harrington A; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Hentges KE; Division of Evolution and Genomic Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK., Humphreys N; Transgenic Unit Core Facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Imai S; Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Ishii H; Department of Medical Data Science, Osaka University Graduate School of Medicine, Suita, Japan., Iwama M; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Jonasch E; The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Karolak M; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Keavney B; Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester and Manchester Heart Centre, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK., Khin NC; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Konno M; Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Suita, Japan., Kotani Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Kunihiro Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Lakshmanan I; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Larochelle C; Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Lawrence CB; Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK., Li L; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Lindner V; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Liu XD; The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Lopez-Castejon G; Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK., Loudon A; Centre for Biological Timing, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Lowe J; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Jerome-Majewska LA; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Matsusaka T; Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Miura H; Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Miyasaka Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Morpurgo B; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Motyl K; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Nabeshima YI; Laboratory of Molecular Life Science, Foundation for Biomedical Research and Innovation, Kobe, Japan., Nakade K; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Nakashiba T; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Nakashima K; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Obata Y; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Ogiwara S; Department of Laboratory Animal Science, Support Center for Medical Research and Education, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Ouellet M; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Oxburgh L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA.; Basic and Clinical Research, The Rogosin Institute, New York, USA., Piltz S; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., Pinz I; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Ponnusamy MP; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA., Ray D; Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, OX37LE, UK., Redder RJ; High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA., Rosen CJ; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Ross N; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Ruhe MT; Mouse Biology Program, University of California, Davis, USA., Ryzhova L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Salvador AM; Department of Immunology, Tufts University School of Medicine, Boston, USA., Alam SS; Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada., Sedlacek R; Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic., Sharma K; College of Osteopathic Medicine, Marian University, Indianapolis, IN, 46222, USA., Smith C; The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Staes K; Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Starrs L; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia., Sugiyama F; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Takahashi S; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Tanaka T; Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan., Trafford AW; Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK., Uno Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Vanhoutte L; Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Vanrockeghem F; Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Willis BJ; Mouse Biology Program, University of California, Davis, USA., Wright CS; School of Health and Human Sciences, Department of Physical Therapy, Indiana University, Indianapolis, IN, 46202, USA., Yamauchi Y; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Yi X; School of Health and Human Sciences, Department of Physical Therapy, Indiana University, Indianapolis, IN, 46202, USA., Yoshimi K; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Zhang X; The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Zhang Y; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Ohtsuka M; Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Das S; Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, MN, USA., Garry DJ; Paul and Sheila Wellstone Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN, USA.; Department of Surgery, School of Medicine, University of California, Davis, Davis, USA., Hochepied T; Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Thomas P; South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia., Parker-Thornburg J; The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Adamson AD; Transgenic Unit Core Facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Yoshiki A; RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan., Schmouth JF; Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada., Golovko A; Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA., Thompson WR; School of Health and Human Sciences, Department of Physical Therapy, Indiana University, Indianapolis, IN, 46202, USA., Lloyd KCK; Mouse Biology Program, University of California, Davis, USA.; Department of Surgery, School of Medicine, University of California, Davis, Davis, USA., Wood JA; Mouse Biology Program, University of California, Davis, USA., Cowan M; McGill Integrated Core for Animal Modeling (MICAM), Montreal, Canada., Mashimo T; The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan., Mizuno S; Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan., Zhu H; Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA., Kasparek P; Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic., Liaw L; Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA., Miano JM; University of Rochester Medical Center, Rochester, NY, 14642, USA., Burgio G; Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia. gaetan.burgio@anu.edu.au.

Publikováno v: Genome biology [Genome Biol] 2019 Aug 26; Vol. 20 (1), pp. 171. Date of Electronic Publication: 2019 Aug 26.

Antiretroviral Drug Metabolism in Humanized PXR-CAR-CYP3A-NOG Mice.

Autor: McMillan JM; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska lpoluekt@unmc.edu cgurumurthy@unmc.edu jmmcmillan@unmc.edu., Cobb DA; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Lin Z; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Banoub MG; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Dagur RS; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Branch Woods AA; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Wang W; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Makarov E; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Kocher T; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Joshi PS; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Quadros RM; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Harms DW; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Cohen SM; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Gendelman HE; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Gurumurthy CB; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska lpoluekt@unmc.edu cgurumurthy@unmc.edu jmmcmillan@unmc.edu., Gorantla S; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska., Poluektova LY; Department of Pharmacology and Experimental Neuroscience (J.M.M., D.A.C., M.G.B., R.S.D., A.A.B.W., W.W., E.M., T.K., P.S.J., H.E.G., S.G., L.Y.P.), Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation (C.B.G.), Department of Pharmaceutical Sciences (Z.L.), Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office (R.M.Q., D.W.H., C.B.G.), and Department of Pathology and Microbiology (S.M.C.), University of Nebraska Medical Center, Omaha, Nebraska lpoluekt@unmc.edu cgurumurthy@unmc.edu jmmcmillan@unmc.edu.

Publikováno v: The Journal of pharmacology and experimental therapeutics [J Pharmacol Exp Ther] 2018 May; Vol. 365 (2), pp. 272-280. Date of Electronic Publication: 2018 Feb 23.

Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.

Autor: Jacobi AM; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA., Rettig GR; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA., Turk R; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA., Collingwood MA; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA., Zeiner SA; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA., Quadros RM; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA., Harms DW; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA., Bonthuis PJ; Department of Neurobiology & Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA., Gregg C; Department of Neurobiology & Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA; Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84132, USA., Ohtsuka M; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259-1193, Japan; Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan; The Institute of Medical Sciences, Tokai University, Kanagawa 259-1193, Japan., Gurumurthy CB; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA; Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE 68198, USA. Electronic address: cgurumurthy@unmc.edu., Behlke MA; Integrated DNA Technologies, Inc., Coralville, IA 52241, USA. Electronic address: mbehlke@idtdna.com.

Publikováno v: Methods (San Diego, Calif.) [Methods] 2017 May 15; Vol. 121-122, pp. 16-28. Date of Electronic Publication: 2017 Mar 27.