Targeted locus amplification to develop robust patient-specific assays for liquid biopsies in pediatric solid tumors

Autor: Lieke M. J. van Zogchel, Nathalie S. M. Lak, Nina U. Gelineau, Irina Sergeeva, Ellen Stelloo, Joost Swennenhuis, Harma Feitsma, Max van Min, Erik Splinter, Margit Bleijs, Marian Groot Koerkamp, Willemijn Breunis, Michael Torsten Meister, Waleed Hassan Kholossy, Frank C. P. Holstege, Jan J. Molenaar, Wendy W. J. de Leng, Janine Stutterheim, C. Ellen van der Schoot, Godelieve A. M. Tytgat

Publikováno v: Frontiers in Oncology, Vol 13 (2023)

BackgroundLiquid biopsies combine minimally invasive sample collection with sensitive detection of residual disease. Pediatric malignancies harbor tumor-driving copy number alterations or fusion genes, rather than recurrent point mutations. These reg

Externí odkaz: https://doaj.org/article/7b442cccd01346f9baef8bf01b4fb357

An ENU-mutagenesis screen in the mouse: identification of novel developmental gene functions.

Autor: Carolien Wansleeben, Léon van Gurp, Harma Feitsma, Carla Kroon, Ester Rieter, Marlies Verberne, Victor Guryev, Edwin Cuppen, Frits Meijlink

Publikováno v: PLoS ONE, Vol 6, Iss 4, p e19357 (2011)

BACKGROUND: Mutagenesis screens in the mouse have been proven useful for the identification of novel gene functions and generation of interesting mutant alleles. Here we describe a phenotype-based screen for recessive mutations affecting embryonic de

Externí odkaz: https://doaj.org/article/5139dec19ac9416e8d5e82a3792a9c57

Minimal residual disease (MRD) detection in acute lymphoblastic leukaemia based on fusion genes and genomic deletions

Autor: Monique L. den Boer, Harma Feitsma, Reno Bladergroen, Edwin Sonneveld, Roland P. Kuiper, Vincent H.J. van der Velden, Irina Sergeeva, Freerk van Dijk, Judith M. Boer, Frank N. van Leeuwen, P G Hoogeveen

Publikováno v: British Journal of Haematology, 194(5), 888-892. Wiley-Blackwell Publishing Ltd

Minimal residual disease (MRD) diagnostics are implemented in most clinical protocols for patients with acute lymphoblastic leukaemia (ALL) and are mostly performed using rearranged immunoglobulin (IG) and/or T-cell receptor (TR) gene rearrangements

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a71318a0b59cb5d52c1e12bcdf5db2a3
https://pure.eur.nl/en/publications/046d984b-cc3e-4ecd-8e53-5c44262337af

Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing

Autor: Joost Vermaat, Tom van Wezel, Paula J P de Vree, Wouter de Laat, Bauke Ylstra, Amin Allahyar, Marieke Simonis, Harma Feitsma, Adrien S. J. Melquiond, Max van Min, Agata Rakszewska, Erik Splinter, Daphne de Jong, Joost Swennenhuis, Milan Sharma, Mehmet Yilmaz, Arjan Diepstra, Roos J Leguit, Robert van der Geize, Phylicia Stathi, Karima Hajo, Nathalie J. Hijmering, Mark Pieterse, Marjon J.A.M. Verstegen, Peter H.L. Krijger, Ruud W J Meijers, G Tjitske Los-de Vries, Léon C van Kempen, Arjen H.G. Cleven

Publikováno v: Nature Communications, 12(1):3361. Nature Publishing Group
Nature Communications
Nature Communications, 12(1). Nature Publishing Group
Nature Communications, 12(1). NATURE PORTFOLIO
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Allahyar, A, Pieterse, M, Swennenhuis, J, Los-de Vries, G T, Yilmaz, M, Leguit, R, Meijers, R W J, van der Geize, R, Vermaat, J, Cleven, A, van Wezel, T, Diepstra, A, van Kempen, L O C, Hijmering, N J, Stathi, P, Sharma, M, Melquiond, A S J, de Vree, P J P, Verstegen, M J A M, Krijger, P H L, Hajo, K, Simonis, M, Rakszewska, A, van Min, M, de Jong, D, Ylstra, B, Feitsma, H, Splinter, E & de Laat, W 2021, ' Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing ', Nature Communications, vol. 12, no. 1, 3361 . https://doi.org/10.1038/s41467-021-23695-8

In routine diagnostic pathology, cancer biopsies are preserved by formalin-fixed, paraffin-embedding (FFPE) procedures for examination of (intra-) cellular morphology. Such procedures inadvertently induce DNA fragmentation, which compromises sequenci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f015170cc76e934c99a06da52084f3ba
https://research.vumc.nl/en/publications/dbd7c41c-2950-4947-b268-32207673d8fd

Abstract 2229: Targeted locus amplification based sequencing for mapping viral integration sites in human papillomavirus positive head and neck squamous cell carcinomas

Autor: Imke Demers, Harini Balaji, Harma Feitsma, Irinia Sergeeva, Joost Swennenhuis, Nora Wuerdemann, Steffen Wagner, Bernd Kremer, Christian Huebbers, Jens Peter Klussmann, Ernst-Jan Speel

Publikováno v: Cancer Research. 82:2229-2229

Background: Human papillomavirus (HPV) infections are the principal cause of cervical cancers, subsets of anogenital and head and neck cancers (HNC). During persistent infection, viral DNA integration into the host genome may occur, which is suggeste

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::97ff75ffef1c6c4e699909bffb6a723e
https://doi.org/10.1158/1538-7445.am2022-2229