Zobrazeno 1 - 10 of 13 pro vyhledávání: '"Harley King"'
1
Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin
Autor: Daniel C. Nelson, Sowmya Ajay Castro, Helge C. Dorfmueller, Harley King, Amol Arunrao Pohane, Natalia Korotkova, C. M. Scholte, Vincent A. Fischetti
Publikováno v: Biochem J
Endolysins are peptidoglycan (PG) hydrolases that function as part of the bacteriophage (phage) lytic system to release progeny phage at the end of a replication cycle. Notably, endolysins alone can produce lysis without phage infection, which offers
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2daf9529d7f90efa73e1c4c98f6bccb7
https://doi.org/10.1042/bcj20210158
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3
Lab 5--Cloning a PCR Fragment into a Plasmid (Paper Activity) v1
Autor: Harley King
This activity includes a pdf containing instructions for creating a paper plasmid and PCR fragment (gene of interest). I remember doing this with undergraduates with another professor but I couldn't find a similiar activity online. So I recreated it.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c1dbe8962a217465ff6bf901df1a02ad
https://doi.org/10.17504/protocols.io.bmytk7wn
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4
Quantifying Fluorescent Cells in Mammalian Cell Tissue Culture v1
Autor: Harley King
Quantifying the change in fluorescent status of cells over time can be an important data point in experimental analysis. Optimally, quantification can be accomplished with flow cytometers. However, these instruments are not available to every lab. Ro
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b7c26b0b012bfb868c7cfd49f4223c5f
https://doi.org/10.17504/protocols.io.y2nfyde
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5
Purification of Haustoria from Arabidopsis in Response to Infection by E. cichoracearum v1
Autor: Harley King
This procedure describes steps for the purification of Arabidopthis thaliana haustoria in response to powdery mildew infection by fungus Golovinomyces cichoracearum.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aa588c5a89b3573a7266f93811eefcf0
https://doi.org/10.17504/protocols.io.tqkemuw
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6
Using a Peristaltic Pump to Flow Buffer Through a Nanoporous Membrane in Filter Holder Assembly v1
Autor: Harley King
This protocol provides steps for seating and flowing liquid through a 50 μm, 13mm round membrane containing a hexagonal arrangement of 25nm pores with lattice constant 65nm using a peristaltic pump.Porous, alumina membranes are fragile and pressure
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3dc841ec512f423a03f377977436ec88
https://doi.org/10.17504/protocols.io.rwqd7dw
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7
Expression and Purification of TailSpike 1 (TSP1) Protein from E. coli v1
Autor: Harley King
This protocoll adds greater clarity and stepwise-discriptions regarding the purification of tailspike protein 1 (TSP1). The structure of TSP1 is 4OJ5.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::25557e0fba67004bf260ef3945c2dae0
https://doi.org/10.17504/protocols.io.pikdkcw
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10
Book Review: Essentials of pharmacoeconomics
Autor: Harley King
Publikováno v: Journal of Commercial Biotechnology. 15
Essentials of pharmacoeconomicsKaren L. RascatiLippincott Williams & Wilkins, Baltimore, 2008, paperback, 250pp.,ISBN: 978-0781765442
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f4ddf7c5ca7055ed5336b903f43b4fd6
https://doi.org/10.5912/jcb275
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