Zobrazeno 1 - 10
of 70
pro vyhledávání: '"Harald von Melchner"'
Publikováno v:
Disease Models & Mechanisms, Vol 14, Iss 2 (2021)
Gene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells. It is performed with generic gene trap vectors that simultaneously mutate and report the expression
Externí odkaz:
https://doaj.org/article/bff5a3143f874eed9933fe8a8a39924a
Autor:
Chiara Cencioni, Francesco Spallotta, Matteo Savoia, Carsten Kuenne, Stefan Guenther, Agnese Re, Susanne Wingert, Maike Rehage, Duran Sürün, Mauro Siragusa, Jacob G. Smith, Frank Schnütgen, Harald von Melchner, Michael A. Rieger, Fabio Martelli, Antonella Riccio, Ingrid Fleming, Thomas Braun, Andreas M. Zeiher, Antonella Farsetti, Carlo Gaetano
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
The production of nitric oxide (NO) is required for early stage embryo implantation into the uterus. Here the authors show that during differentiation of naive mouse ESCs, early production of endogenous NO leads to a mesendoderm differentiation commi
Externí odkaz:
https://doaj.org/article/a14bcea599a241e4b7bb31b5f8b2ac2b
Autor:
Duran Sürün, Joachim Schwäble, Ana Tomasovic, Roy Ehling, Stefan Stein, Nina Kurrle, Harald von Melchner, Frank Schnütgen
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 10, Iss , Pp 1-8 (2018)
The CRISPR/Cas9 prokaryotic adaptive immune system and its swift repurposing for genome editing enables modification of any prespecified genomic sequence with unprecedented accuracy and efficiency, including targeted gene repair. We used the CRISPR/C
Externí odkaz:
https://doaj.org/article/1fd511876df643e782f4fb93c325e28b
Autor:
Alexander Strassman, Frank Schnütgen, Qi Dai, Jennifer C. Jones, Angela C. Gomez, Lenore Pitstick, Nathan E. Holton, Russell Moskal, Erin R. Leslie, Harald von Melchner, David R. Beier, Bryan C. Bjork
Publikováno v:
Disease Models & Mechanisms, Vol 10, Iss 7, Pp 909-922 (2017)
Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays t
Externí odkaz:
https://doaj.org/article/6b4e3b20140d48539758e197974877ee
Autor:
Insa Bultmann-Mellin, Jeroen Essers, Paula M. van Heijingen, Harald von Melchner, Gerhard Sengle, Anja Sterner-Kock
Publikováno v:
Disease Models & Mechanisms, Vol 9, Iss 11, Pp 1367-1374 (2016)
LTBP-4L and LTBP-4S are two isoforms of the extracellular matrix protein latent-transforming growth factor beta-binding protein 4 (LTBP-4). The mutational inactivation of both isoforms causes autosomal recessive cutis laxa type 1C (ARCL1C) in humans
Externí odkaz:
https://doaj.org/article/57e40f79a57649e39df7b3cfe04bce2c
Autor:
Uta Rabenhorst, Frederic B. Thalheimer, Katharina Gerlach, Marek Kijonka, Stefanie Böhm, Daniela S. Krause, Franz Vauti, Hans-Henning Arnold, Timm Schroeder, Frank Schnütgen, Harald von Melchner, Michael A. Rieger, Martin Zörnig
Publikováno v:
Cell Reports, Vol 11, Iss 12, Pp 1847-1855 (2015)
The ability of hematopoietic stem cells (HSCs) to self-renew is a prerequisite for the establishment of definitive hematopoiesis and life-long blood regeneration. Here, we report the single-stranded DNA-binding transcriptional regulator far upstream
Externí odkaz:
https://doaj.org/article/c1163f6eebe147a3bd1e930b1679b3f9
Autor:
Insa Bultmann-Mellin, Anne Conradi, Alexandra C. Maul, Katharina Dinger, Frank Wempe, Alexander P. Wohl, Thomas Imhof, F. Thomas Wunderlich, Alexander C. Bunck, Tomoyuki Nakamura, Katri Koli, Wilhelm Bloch, Alexander Ghanem, Andrea Heinz, Harald von Melchner, Gerhard Sengle, Anja Sterner-Kock
Publikováno v:
Disease Models & Mechanisms, Vol 8, Iss 4, Pp 403-415 (2015)
Recent studies have revealed an important role for LTBP-4 in elastogenesis. Its mutational inactivation in humans causes autosomal recessive cutis laxa type 1C (ARCL1C), which is a severe disorder caused by defects of the elastic fiber network. Altho
Externí odkaz:
https://doaj.org/article/a4bd9f218f2d4e76a01d420ae7b52376
Autor:
Juliana Heidler, Athanasios Fysikopoulos, Frank Wempe, Michael Seimetz, Thorsten Bangsow, Ana Tomasovic, Florian Veit, Susan Scheibe, Alexandra Pichl, Friederike Weisel, K. C. Kent Lloyd, Peter Jaksch, Walter Klepetko, Norbert Weissmann, Harald von Melchner
Publikováno v:
Disease Models & Mechanisms, Vol 6, Iss 6, Pp 1378-1387 (2013)
SUMMARY Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. COPD is caused by chronic exposure to cigarette smoke and/or other environmental pollutants that are believed to induce reactive oxygen spec
Externí odkaz:
https://doaj.org/article/d0798f2f4197457a84ffe11f8b2917d4
Publikováno v:
Drug Discovery Today: Technologies. 28:33-39
The development of genome editing tools capable of modifying specific genomic sequences with unprecedented accuracy has opened up a wide range of new possibilities in targeted gene manipulation. In particular, the CRISPR/Cas9 system, a repurposed pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d98623f1272edc9af34e4e1e8fe0edd9
https://doi.org/10.1016/j.ddtec.2018.08.001
https://doi.org/10.1016/j.ddtec.2018.08.001