Disarming Staphylococcus aureus from destroying human cells by simultaneously neutralizing six cytotoxins with two human monoclonal antibodies

Autor: Harald Rouha, Susanne Weber, Philipp Janesch, Barbara Maierhofer, Karin Gross, Ivana Dolezilkova, Irina Mirkina, Zehra C. Visram, Stefan Malafa, Lukas Stulik, Adriana Badarau, Eszter Nagy

Publikováno v: Virulence, Vol 9, Iss 1, Pp 231-247 (2018)

Pathogenesis of Staphylococcus aureus is increasingly recognized to be driven by powerful toxins. Staphylococcus aureus employs up to six pore-forming toxins to subvert the human host defense and to promote bacterial invasion: alpha-hemolysin that di

Externí odkaz: https://doaj.org/article/bffc02e8566b438db1748413ad61e559

Assessing the function of pneumococcal neuraminidases NanA, NanB and NanC in in vitro and in vivo lung infection models using monoclonal antibodies

Autor: Lukas Stulik, Nels Nielson, Marisa Caccamo, Eszter Nagy, Christine A. Power, Ivana Dolezilkova, Adriana Badarau, Karin Groß, Gerhild Zauner, Irina Mirkina, Philipp Janesch, Sabine Maier, Manuel Zerbs, Jacqueline Steinhäuser, Cecília Varga, Katharina Havlicek, Susanne Maria Weber, Barbara Maierhofer, Harald Rouha

Publikováno v: Virulence
Virulence, Vol 9, Iss 1, Pp 1521-1538 (2018)

Streptococcus pneumoniae isolates express up to three neuraminidases (sialidases), NanA, NanB and NanC, all of which cleave the terminal sialic acid of glycan-structures that decorate host cell surfaces. Most research has focused on the role of NanA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0eedca08714bb46ebf828909b0dc2395

Molecular mechanism of leukocidin GH–integrin CD11b/CD18 recognition and species specificity

Autor: Dmitri I. Svergun, Harald Rouha, Kristina Djinović-Carugo, Adriana Badarau, Nikolina Trstenjak, Melissa A. Graewert, Eszter Nagy, Dalibor Milić

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America 117(1), 317-327 (2020). doi:10.1073/pnas.1913690116
Proceedings of the National Academy of Sciences of the United States of America

Significance Staphylococcus aureus is one of the most virulent bacterial pathogens and, in particular, has the richest repertoire of cytotoxins: A single bacterium can secrete 6 different β-barrel pore-forming toxins, with different cell type and sp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b4ab056a76ec50eed87751a78f2ea9f
https://www.bib.irb.hr/1041659

Selective sensitization of human neutrophils to LukGH mediated cytotoxicity by Staphylococcus aureus and IL-8

Autor: Lukas Stulik, Harald Rouha, Adriana Badarau, Susanne Maria Weber, Karin Gross, Zehra Visram, Barbara Maierhofer, Philipp Janesch, Stefan Malafa, Eszter Nagy

Publikováno v: Journal of Infection. 74:473-483

Summary Objectives Staphylococcus aureus produces up to five bi-component leukocidins – LukSF-PV, gamma-hemolysins AB and CB, LukGH (LukAB) and LukED – to evade innate immunity by lysing phagocytic cells. Species specificity of these leukocidins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7b1fb4c5f0144741e195486d642dc94
https://doi.org/10.1016/j.jinf.2017.02.004

Preventing lung pathology and mortality in rabbit Staphylococcus aureus pneumonia models with cytotoxin-neutralizing monoclonal IgGs penetrating the epithelial lining fluid

Autor: Delphine Croisier, Adriana Badarau, Susanne Maria Weber, Delphine Labrousse, Ines Glojnarić, Miroslava Dominis Kramarić, Zehra Visram, Eszter Nagy, Barbara Maierhofer, Harald Rouha, Karin Groß, Lukas Stulik, Gabor Nagy

Publikováno v: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)

Staphylococcus aureus pneumonia is associated with high mortality irrespective of antibiotic susceptibility. Both MRSA and MSSA strains produce powerful cytotoxins: alpha-hemolysin(Hla) and up to five leukocidins – LukSF-PV, HlgAB, HlgCB, LukED and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cb05f2df331f5b4b0f833e913e567fc
https://doi.org/10.1038/s41598-019-41826-6