Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Harald Putzer"'
Publikováno v:
International Journal of Molecular Sciences, Vol 25, Iss 15, p 8537 (2024)
RNase Y is a key endoribonuclease that regulates global mRNA turnover and processing in Bacillus subtilis and likely many other bacteria. This enzyme is anchored to the cell membrane, creating a pseudo-compartmentalization that aligns with its role i
Externí odkaz:
https://doaj.org/article/b703f36ff9824636af524aebfb4854f9
Publikováno v:
Microorganisms, Vol 11, Iss 6, p 1374 (2023)
The instability of messenger RNA is crucial to the control of gene expression. In Bacillus subtilis, RNase Y is the major decay-initiating endoribonuclease. Here, we show how this key enzyme regulates its own synthesis by modulating the longevity of
Externí odkaz:
https://doaj.org/article/f3172aa19a9e4f76b132ae352728483d
Publikováno v:
Frontiers in Microbiology, Vol 11 (2020)
mRNA levels result from an equilibrium between transcription and degradation. Ribonucleases (RNases) facilitate the turnover of mRNA, which is an important way of controlling gene expression, allowing the cells to adjust transcript levels to a changi
Externí odkaz:
https://doaj.org/article/e5bb7f58380a4d5fa6f813a005f5570e
Autor:
Lina Hamouche, Cyrille Billaudeau, Anna Rocca, Arnaud Chastanet, Saravuth Ngo, Soumaya Laalami, Harald Putzer
Publikováno v:
mBio, Vol 11, Iss 1 (2020)
ABSTRACT Metabolic turnover of mRNA is fundamental to the control of gene expression in all organisms, notably in fast-adapting prokaryotes. In many bacteria, RNase Y initiates global mRNA decay via an endonucleolytic cleavage, as shown in the Gram-p
Externí odkaz:
https://doaj.org/article/56d807b040944a519243fead5a93f385
Autor:
Lina Hamouche, Soumaya Laalami, Adrian Daerr, Solène Song, I. Barry Holland, Simone J. Séror, Kassem Hamze, Harald Putzer
Publikováno v:
mBio, Vol 8, Iss 1 (2017)
ABSTRACT Bacteria adopt social behavior to expand into new territory, led by specialized swarmers, before forming a biofilm. Such mass migration of Bacillus subtilis on a synthetic medium produces hyperbranching dendrites that transiently (equivalent
Externí odkaz:
https://doaj.org/article/5271839ea886466e849fbb3edf6d35d9
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e63025 (2013)
B. subtilis grows more rapidly using the amino sugar glucosamine as carbon source, than with N-acetylglucosamine. Genes for the transport and metabolism of N-acetylglucosamine (nagP and nagAB) are found in all the sequenced Bacilli (except Anoxybacil
Externí odkaz:
https://doaj.org/article/efafedba40c14875ac46bf588cfee35d
Autor:
Rebecca Schroeter, Tamara Hoffmann, Birgit Voigt, Hanna Meyer, Monika Bleisteiner, Jan Muntel, Britta Jürgen, Dirk Albrecht, Dörte Becher, Michael Lalk, Stefan Evers, Johannes Bongaerts, Karl-Heinz Maurer, Harald Putzer, Michael Hecker, Thomas Schweder, Erhard Bremer
Publikováno v:
PLoS ONE, Vol 8, Iss 11, p e80956 (2013)
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats
Externí odkaz:
https://doaj.org/article/2e1f0b83c78041f292744332716d8c23
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e54062 (2013)
RNase Y is a key endoribonuclease affecting global mRNA stability in Bacillus subtilis. Its characterization provided the first evidence that endonucleolytic cleavage plays a major role in the mRNA metabolism of this organism. RNase Y shares importan
Externí odkaz:
https://doaj.org/article/2942a30ec0664c13a27ec4a2443f3f6d
Autor:
Harald Putzer
Publikováno v:
Bio-Protocol, Vol 2, Iss 14 (2012)
5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases gene
Externí odkaz:
https://doaj.org/article/86625865d451487f95bcda4754651ea0
Autor:
Harald Putzer
Publikováno v:
Bio-Protocol, Vol 2, Iss 14 (2012)
Primer extension analysis is a useful method to determine the transcription start point or a processing site on an RNA molecule. It can also allow a quantitative measurement of an RNA species.
Externí odkaz:
https://doaj.org/article/ab178948a1b64f2db5ddcc8a64e425e6