Zobrazeno 1 - 10 of 44 pro vyhledávání: '"Harald Mikkers"'
1
Akademický článek
Generation and genetic repair of two human induced pluripotent cell lines from patients with Epidermolysis Bullosa simplex and dilated cardiomyopathy associated with a heterozygous mutation in the translation initiation codon of KLHL24
Autor: Veronika Ramovs, Ignacia Fuentes, Christian Freund, Harald Mikkers, Christine L. Mummery, Karine Raymond
Publikováno v: Stem Cell Research, Vol 57, Iss , Pp 102582- (2021)
Fibroblasts from two patients carrying a heterozygous mutation in the translation initiation codon (c.2 T > G) of the kelch-like protein 24 (KLHL24) gene were used to generate human induced pluripotent stem cells (hiPSCs), using non-integrating Senda
Externí odkaz: https://doaj.org/article/4ba238b09fea4e21abaf8fc5682b0447
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2
Akademický článek
Axin2/Conductin Is Required for Normal Haematopoiesis and T Lymphopoiesis
Autor: Jolanda J. D. de Roo, Amiet Chhatta, Laura Garcia-Perez, Brigitta A. E. Naber, Sandra A. Vloemans, Daniela C. F. Salvatori, Karin Pike-Overzet, Harald Mikkers, Frank J. T. Staal
Publikováno v: Cells, Vol 11, Iss 17, p 2679 (2022)
The development of T lymphocytes in the thymus and their stem cell precursors in the bone marrow is controlled by Wnt signaling in strictly regulated, cell-type specific dosages. In this study, we investigated levels of canonical Wnt signaling during
Externí odkaz: https://doaj.org/article/f6fbe9006a224665ace8e4225a694060
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3
Akademický článek
Protocol for Isolation, Stimulation and Functional Profiling of Primary and iPSC-derived Human NK Cells
Autor: Janine Melsen, Maria Themeli, Monique van Ostaijen-ten Dam, Els van Beelen, Gertjan Lugthart, Rob Hoeben, Marco Schilham, Harald Mikkers
Publikováno v: Bio-Protocol, Vol 10, Iss 23 (2020)
Natural killer (NK) cells are innate immune cells, characterized by their cytotoxic capacity, and chemokine and cytokine secretion upon activation. Human NK cells are identified by CD56 expression. Circulating NK cells can be further subdivided into
Externí odkaz: https://doaj.org/article/8044ba1b7bf042f5bb9492094d684369
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4
Akademický článek
Generation and genetic repair of 2 iPSC clones from a patient bearing a heterozygous c.1120del18 mutation in the ACVRL1 gene leading to Hereditary Hemorrhagic Telangiectasia (HHT) type 2
Autor: Marga J. Bouma, Valeria Orlova, Francijna E. van den Hil, Hans-Jurgen Mager, Frank Baas, Peter de Knijff, Christine L. Mummery, Harald Mikkers, Christian Freund
Publikováno v: Stem Cell Research, Vol 46, Iss , Pp 101786- (2020)
Fibroblasts from a patient carrying a heterozygous 18bp deletion in exon 8 of the ACVRL1 gene (c.1120del18) were reprogrammed using episomal vectors. The in-frame deletion in ACVRL1 causes the loss of 6 amino acids of the protein, which is associated
Externí odkaz: https://doaj.org/article/3068a223859c41b490e4ab2d56b63fb8
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5
Akademický článek
Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient
Autor: Elena Daoutsali, Ronald A.M. Buijsen, Simone van de Pas, Anke 't Jong, Harald Mikkers, Tom Brands, Bert Eussen, Annelies de Klein, Linda M. van der Graaf, Barry A. Pepers, Christian Freund, Gisela M. Terwindt, Valeria V. Orlova, Willeke M.C. van Roon-Mom
Publikováno v: Stem Cell Research, Vol 34, Iss , Pp - (2019)
Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type (HCHWA-D) is an autosomal dominant hereditary disease caused by a point mutation in exon 17 of the APP gene. We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic HCHW
Externí odkaz: https://doaj.org/article/95930d38c64a4aaba11dcfaceede4516
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6
Generation of LUMCi041-A-2
Autor: U Chaudhari, A Ranga, Christiaan H. Arendzen, Christian Freund, O Pourquie, Harald Mikkers, Christine L. Mummery, S J Cramer
Publikováno v: Stem Cell Research, Vol 57, Iss, Pp 102592-(2021)
Stem Cell Research, 57. ELSEVIER
An induced pluripotent stem cell (iPSC) line, in which a H2B-fluorescent protein fusion is temporally expressed, is a valuable tool to track cells and study cell divisions and apoptosis. To this end we introduced a 3rd generation "all-in-one" doxycyc
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d315ab315879becb2125f470c0dab47
https://hdl.handle.net/1887/3276395
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7
In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells
Autor: Christine L. Mummery, Aat A. Mulder, Carolina R. Jost, André A. Poot, Teresa D. Tetley, Thijs Pasman, Danielle Baptista, Pieter S. Hiemstra, Dennis K. Ninaber, Sander van Riet, Roman Truckenmüller, Robbert J. Rottier, Christian Freund, Harald Mikkers
Publikováno v: Scientific reports, 10(1):5499. Nature Publishing Group
Scientific Reports
Scientific Reports, 10(1):5499. Nature Publishing Group
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports, 10(1). NATURE PUBLISHING GROUP
Research on acute and chronic lung diseases would greatly benefit from reproducible availability of alveolar epithelial cells (AEC). Primary alveolar epithelial cells can be derived from human lung tissue but the quality of these cells is highly dono
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e7823141af10b77bb297829d4540bd0
http://www.scopus.com/inward/record.url?scp=85082560840&partnerID=8YFLogxK
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8
Reprogramming Urine-Derived Cells using Commercially Available Self-Replicative RNA and a Single Electroporation
Autor: Marga J. Bouma, Christine L. Mummery, Christiaan H. Arendzen, Christian Freund, Harald Mikkers
Publikováno v: Current Protocols in Stem Cell Biology
We describe a protocol for efficient generation of human‐induced pluripotent stem cells (hiPSCs) from urine‐derived cells (UDCs) obtained from adult donors using self‐replicative RNA containing the reprogramming factors OCT3/4, SOX2, KLF4, GLIS
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb982b3100272b6f75a7a349fcd2914
https://pubmed.ncbi.nlm.nih.gov/32956580
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10
iPSC-based modeling of RAG2 severe combined immunodeficiency reveals multiple T cell developmental arrests
Autor: Rob C. Hoeben, Amiet R. Chhatta, Maria Themeli, Edwin de Wilt, Hester Boersma, Aïda Shahrabi Farahani, Martijn Cordes, Henk-Jan Prins, Bart Vandekerckhove, Frank J. T. Staal, Harald Mikkers, Mirjam van der Burg
Publikováno v: Stem Cell Reports, Vol 14, Iss 2, Pp 300-311 (2020)
STEM CELL REPORTS
Stem Cell Reports
Themeli, M, Chhatta, A, Boersma, H, Prins, H J, Cordes, M, de Wilt, E, Farahani, A S, Vandekerckhove, B, van der Burg, M, Hoeben, R C, Staal, F J T & Mikkers, H M M 2020, ' iPSC-Based Modeling of RAG2 Severe Combined Immunodeficiency Reveals Multiple T Cell Developmental Arrests ', Stem Cell Reports, vol. 14, no. 2, pp. 300-311 . https://doi.org/10.1016/j.stemcr.2019.12.010
Stem Cell Reports, 14(2), 300-311. CELL PRESS
Stem Cell Reports, 14(2), 300-311. Cell Press
Summary RAG2 severe combined immune deficiency (RAG2-SCID) is a lethal disorder caused by the absence of functional T and B cells due to a differentiation block. Here, we generated induced pluripotent stem cells (iPSCs) from a RAG2-SCID patient to st
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c14e958e4b23f7f4aa64dd4958988485
https://hdl.handle.net/1887/3182641
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