Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Harald, Wegele"'
Autor:
Sanjay K. Gupta, Tobias Graf, Franziska T. Edelmann, Helen Seelmann, Markus Reintinger, Lars Hillringhaus, Frank Bergmann, Michael Wiedmann, Roberto Falkenstein, Harald Wegele, Inn H. Yuk, Michael Leiss
Publikováno v:
European Journal of Pharmaceutics and Biopharmaceutics. 187:120-129
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6f14b2ffff50809d2441c373d1bab989
https://doi.org/10.1016/j.ejpb.2023.04.021
https://doi.org/10.1016/j.ejpb.2023.04.021
Autor:
Felix Kuhne, Katrin Heinrich, Martin Winter, Jürgen Fichtl, Gabriel Hoffmann, Franziska Zähringer, Katharina Spitzauer, Monika Meier, Tarik A. Khan, Lea Bonnington, Katharina Wagner, Jan Olaf Stracke, Dietmar Reusch, Harald Wegele, Michael Mormann, Patrick Bulau
Publikováno v:
Analytical Chemistry. 95:2203-2212
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::331b03c39357f6720c2a66af938caf48
https://doi.org/10.1021/acs.analchem.2c03099
https://doi.org/10.1021/acs.analchem.2c03099
Autor:
Christina Seisenberger, Tobias Graf, Sarah Sticht, Markus Haindl, Ulrich Mohn, Harald Wegele, Michael Wiedmann, Stefanie Wohlrab
Publikováno v:
Biotechnology and bioengineeringREFERENCES. 120(1)
Host cell proteins (HCPs) are inevitable process-related impurities in biotherapeutics commonly monitored by enzyme-linked immunosorbent assays (ELISAs). Of particular importance for their reliable detection are the anti-HCP polyclonal antibodies (pA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3537e19711f7abf13580d0e158c7b047
https://pubmed.ncbi.nlm.nih.gov/36251621
https://pubmed.ncbi.nlm.nih.gov/36251621
Autor:
Felix Kuhne, Lea Bonnington, Sebastian Malik, Marco Thomann, Cecile Avenal, Florian Cymer, Harald Wegele, Dietmar Reusch, Michael Mormann, Patrick Bulau
Publikováno v:
Antibodies, Vol 8, Iss 4, p 49 (2019)
The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various st
Externí odkaz:
https://doaj.org/article/b20b45dd96e84e63b3d0c7384e3f1df2
Autor:
Jürgen Fichtl, Felix Kuhne, Rainer Lang, Harald Wegele, Patrick Bulau, Lea Bonnington, Dietmar Reusch, Markus Haberger, Anna-Katharina Heidenreich, Michaela Hook, Mahdi Adibzadeh, Florian Cymer
Publikováno v:
Journal of the American Society for Mass Spectrometry. 32:2062-2071
The aim of this study was to characterize the product variants of a therapeutic T-cell bispecific humanized monoclonal antibody (TCB Mab, ∼200 kDa, asymmetric) and to develop an online cation-exchange chromatography native electrospray mass spectro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2933266ff4e346e4b66752323a0e13f
https://doi.org/10.1021/jasms.0c00446
https://doi.org/10.1021/jasms.0c00446
Autor:
Harald Wegele, Michael Wiedmann, Christina Seisenberger, Tobias Graf, Stefanie Wohlrab, Markus Haindl
Publikováno v:
Biotechnology and Bioengineering. 118:1116-1126
Host cell proteins (HCPs) constitute a major class of process-related impurities, whose substantial clearance must be demonstrated by suitable analytical methods to warrant product quality and reduce potential safety risks for patients. In this regar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef1f373e352a2e24a02460a8569ec0bb
https://doi.org/10.1002/bit.27635
https://doi.org/10.1002/bit.27635
Autor:
Christina Seisenberger, Tobias Graf, Markus Haindl, Harald Wegele, Michael Wiedmann, Stefanie Wohlrab
Publikováno v:
Biotechnology Progress. 38
In the control strategy for process related impurities in biopharmaceuticals, the enzyme linked immunosorbent assay (ELISA) is the method of choice for the quantification of host cell proteins (HCPs). Besides two dimensional-western blots (2D-WB), th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a221171c0311f4266cd5126b8d99990f
https://doi.org/10.1002/btpr.3244
https://doi.org/10.1002/btpr.3244
Autor:
Katrin Bomans, Antje Lang, Veronika Roedl, Lisa Adolf, Kyrillos Kyriosoglou, Katharina Diepold, Gabriele Eberl, Michael Mølhøj, Ulrike Strauss, Christian Schmalz, Rudolf Vogel, Dietmar Reusch, Harald Wegele, Michael Wiedmann, Patrick Bulau
Publikováno v:
PLoS ONE, Vol 8, Iss 11, p e81639 (2013)
Biotherapeutics are often produced in non-human host cells like Escherichia coli, yeast, and various mammalian cell lines. A major focus of any therapeutic protein purification process is to reduce host cell proteins to an acceptable low level. In th
Externí odkaz:
https://doaj.org/article/23d5cfa594f7468d982b8dc7374de449
Autor:
Hermann Beck, Tilman Schlothauer, Cecile Avenal, Florian Cymer, Daniel Gygax, Harald Wegele, Marco Thomann
Publikováno v:
Biologicals. 50:125-128
Oxidation of monoclonal therapeutic antibodies (mAbs) can affect binding to Fc-receptors and potentially influence pharmacokinetics or effector functions like e.g. antibody dependent cellular phagocytosis (ADCP). Recently, it has been demonstrated th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00dda8d2678b6f1b52baf1333e5471cf
https://doi.org/10.1016/j.biologicals.2017.09.006
https://doi.org/10.1016/j.biologicals.2017.09.006
Autor:
Barbara Entler, Christof Finkler, Valentina Locatelli, Jan Olaf Stracke, Steffen Kiessig, Michele Niess, Andrea Bathke, Bernd Moritz, Harald Wegele
Publikováno v:
Electrophoresis
CZE is a well‐established technique for charge heterogeneity testing of biopharmaceuticals. It is based on the differences between the ratios of net charge and hydrodynamic radius. In an extensive intercompany study, it was recently shown that CZE
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd7a6750818b6ed41bd4ba72a1004c38
https://doi.org/10.1002/elps.201700145
https://doi.org/10.1002/elps.201700145